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Mutation Information
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Mutation Site
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R228A |
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Mutation Type
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Amino acid level |
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Gene/Protein/Region Type
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IN |
Literature Information
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PubMed PMID
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33980829
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Published Year
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2021 |
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Journal
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Nature communications |
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Title
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INI1/SMARCB1 Rpt1 domain mimics TAR RNA in binding to integrase to facilitate HIV-1 replication. |
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Author
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Dixit U,Bhutoria S,Wu X,Qiu L,Spira M,Mathew S,Harris R,Adams LJ,Cahill S,Pathak R,Rajesh Kumar P,Nguyen M,Acharya SA,Brenowitz M,Almo SC,Zou X,Steven AC,Cowburn D,Girvin M,Kalpana GV |
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Evidence
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To test this hypothesis, we determined if: (i) INI1183-304 and TAR compete with each other for binding to IN in vitro; (ii) IN-CTD mutations affect the binding of INI1183-304 and TAR to the same extent; (iii) INI1 can compete with IN binding to TAR in vivo; and (iv) INI1-interaction defective W235E and R228A mutant viruses form morphologically defective particles and are defective for incorporation of INI1 into the virions.
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HIV Mutation Detail Information