| Evidence Sentence: |
To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model. |
| Evidence Sentence: |
In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression. |
| Evidence Sentence: |
The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. |
| Evidence Sentence: |
BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. |
| Evidence Sentence: |
Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA. |
| Evidence Sentence: |
Comparison of the rate of cells positive for BAX mRNA between groups |
| Evidence Sentence: |
As shown in Table 2 and Figure 3, the rate of cells positive for BAX mRNA was significantly higher in the model and acupuncture groups than in the sham surgery groups at the same time points, and the increase was greater in the model groups than in the acupuncture groups. |
| Evidence Sentence: |
No significant differences were found in the rate of cells positive for BAX mRNA between any two subgroups among the 3, 9, 24, and 48 hour model groups. |
| Evidence Sentence: |
In the acupuncture group, the rates of cells positive for BAX mRNA were higher in the 24 hour and 48 hour acupuncture groups than in the 3 hour and 9 hour acupuncture groups (p<0.05), and no significant differences were observed between the 24 hour and 48 hour groups or between the 3 hour and 9 hour groups. |
| Evidence Sentence: |
As shown in Figure 4, BAX mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues after acute cerebral hemorrhage, and the cytoplasm was stained brown or tan. |
| Evidence Sentence: |
Cells positive for BAX mRNA had varied shapes and sizes and were unevenly and sporadically distributed among normal cells. |
| Evidence Sentence: |
Comparison of BAX mRNA levels between groups |
| Evidence Sentence: |
The BAX mRNA levels were significantly higher in the model groups and the acupuncture groups than in the sham groups at the same time point (p<0.05, Table 3 and Figure 6), and the increase in the model groups was more significant than that in the acupuncture groups (p<0.05). |
| Evidence Sentence: |
The 48 hour model group had the highest BAX mRNA levels among all model subgroups, followed in order by the 24, 9, and 3 hour model groups, and the differences between any two of these groups were significant (p<0.05). |
| Evidence Sentence: |
Among all acupuncture subgroups, the 48 hour acupuncture group also showed the highest BAX mRNA levels, followed in order by the 24, 9, and 3 hour groups, and the differences between any two of these groups were significant (p<0.05). |
| Evidence Sentence: |
Comparison of ratios of BCL-2 and BAX mRNA levels between groups |
| Evidence Sentence: |
As shown in Table 3 and Figure 7, the ratio of the BCL-2 mRNA level to the BAX mRNA level was significantly higher in the sham surgery and acupuncture groups than in the model groups at the same time point (p<0.05), with the highest ratio in the acupuncture groups. |
| Evidence Sentence: |
The ratio of BCL-2 mRNA level to BAX mRNA level was significantly higher in the 3 hour and 9 hour model groups compared to the 24 hour and 48 hour model groups (p<0.05), and no significant differences were found between the 3 hour and 9 hour model groups or between the 24 hour and 48 hour model groups. |
| Evidence Sentence: |
The ratio of BCL-2 mRNA level to BAX mRNA level was also significantly higher in the 3 hour and 9 hour acupuncture groups compared to the 24 hour and 48 hour acupuncture groups (p<0.05), and significantly higher in the 24 hour acupuncture group than in the 48 hour group (p<0.05). |
