| Evidence Sentence: |
To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model. |
| Evidence Sentence: |
In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression. |
| Evidence Sentence: |
The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. |
| Evidence Sentence: |
BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. |
| Evidence Sentence: |
Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA. |
| Evidence Sentence: |
Comparison of the number of cells positive for BCL-2 mRNA between groups |
| Evidence Sentence: |
As shown in Table 2 and Figure 1, among all groups, the rate of cells positive for BCL-2 mRNA was significantly higher in the model and acupuncture groups than in the sham surgery groups at the same time point (p<0.05). |
| Evidence Sentence: |
When comparing results within the groups, the rate of cells positive for BCL-2 mRNA was lower in the 3 hour model group than in the other subgroups, and no significant differences were observed among the rest of the subgroups, i.e., the 9 hour model group, 24 hour model group, and 48 hour model group (p<0.05). |
| Evidence Sentence: |
Among the subgroups of the acupuncture group, the rate of cells positive for BCL-2 mRNA was significantly increased in the 3 hour group and 24 hour group compared to the 9 hour and 48 hour groups (p<0.05), and no significant differences were observed between the 3 hour and 24 hour groups or between the 9 hour and 48 hour groups. |
| Evidence Sentence: |
BCL-2 mRNA was expressed at different levels in the cytoplasm in perihematomal brain tissues of rats with acute cerebral hemorrhage, and cytoplasm positive for BCL-2 mRNA exhibited a brown or tan color (Figure 2). |
| Evidence Sentence: |
Cells positive for BCL-2 mRNA were unevenly and sporadically distributed among normal cells, with varied shapes and sizes. |
| Evidence Sentence: |
Comparison of BCL-2 mRNA levels among groups |
| Evidence Sentence: |
BCL-2 mRNA levels were lower in the sham operation group than in the model group, except in the 24 hour and 48 hour model groups, in which the expression of BCL-2 was decreased significantly compared to the sham group (p<0.05, Table 3 and Figure 5). |
| Evidence Sentence: |
Compared to the sham operation and model groups, BCL-2 mRNA levels in the acupuncture groups at the same time points were significantly increased (p<0.05). |
| Evidence Sentence: |
Additionally, BCL-2 mRNA levels in the 3 hour and 9 hour model groups were significantly higher than those in the 24 hour and 48 hour model groups (p<0.05). |
| Evidence Sentence: |
Among the acupuncture subgroups, the BCL-2 mRNA levels were higher (p<0.05) in the 9, 24, and 48 hour groups than in the 3 hour group, but no significant differences were observed among the 9, 24, and 48 hour groups. |
| Evidence Sentence: |
Comparison of ratios of BCL-2 and BAX mRNA levels between groups |
| Evidence Sentence: |
As shown in Table 3 and Figure 7, the ratio of the BCL-2 mRNA level to the BAX mRNA level was significantly higher in the sham surgery and acupuncture groups than in the model groups at the same time point (p<0.05), with the highest ratio in the acupuncture groups. |
| Evidence Sentence: |
The ratio of BCL-2 mRNA level to BAX mRNA level was significantly higher in the 3 hour and 9 hour model groups compared to the 24 hour and 48 hour model groups (p<0.05), and no significant differences were found between the 3 hour and 9 hour model groups or between the 24 hour and 48 hour model groups. |
| Evidence Sentence: |
The ratio of BCL-2 mRNA level to BAX mRNA level was also significantly higher in the 3 hour and 9 hour acupuncture groups compared to the 24 hour and 48 hour acupuncture groups (p<0.05), and significantly higher in the 24 hour acupuncture group than in the 48 hour group (p<0.05). |
