Evidence Details for Calca
PMID Title Journal Year Abstract
26148746 Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats. J Chem Neuroanat. 2015 Oct;68:14-21. doi: 10.1016/j.jchemneu.2015.06.002. Epub 2015 Jul 3. 2015 Oct Previous studies have shown that the effects of manual acupuncture (MA) are contributed by collagen fibers and mast cells in local acupoints, at which acupuncture stimulation causes various afferent fiber groups to be excited. However what happens in local nerve fibers and mast cells after MA remains unclear. The aim of this study was to examine the response of cutaneous nerve fibers and mast cells to MA stimulation in acupoint Hegu (LI4). The contralateral LI4 of the same rat was used as a non-stimulated control. Immnohistochemistry analysis were carried out to observe the expression of histamine (HA), serotonin (5-HT) and nociceptive neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), in the LI4 area. Mast cells were labeled with anti-mast cell tryptase antibody and simultaneously with HA or 5-HT primary antibodies to observe their co-expression. Our results showed that SP and CGRP were expressed more highly on the cutaneous nerve fibers of LI4 after MA stimulation than that of the control. Mast cells aggregated in close proximity to the blood vessels in intra-epidermis and dermis and some of them with degranulation in the lower dermis and subcutaneous tissue of LI4. Both mast cells and their granules appeared with HA (+) and 5-HT (+) expression at stimulated L14 sites, while a few intact mast cells with a little expression of 5-HT and HA were distributed in areas of non-stimulated L14. The results indicated that local cutaneous nerve terminals and mast cells responded to MA with higher expression of SP and CGRP in nerve fibers, as well as with aggregation and degranulation of mast cells with HA and 5-HT granules at acupoint LI4. These neuroactive substances may convey signals to certain pathways that contribute to the effects of acupuncture."

Evidence Sentence: Immnohistochemistry analysis were carried out to observe the expression of histamine (HA), serotonin (5-HT) and nociceptive neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), in the LI4 area.
Evidence Sentence: Our results showed that SP and CGRP were expressed more highly on the cutaneous nerve fibers of LI4 after MA stimulation than that of the control.
Evidence Sentence: The results indicated that local cutaneous nerve terminals and mast cells responded to MA with higher expression of SP and CGRP in nerve fibers,
Evidence Sentence: in which we exposed sensory nerve fibers using substance P (SP) and CGRP,
Evidence Sentence: We hypothesized that acupuncture stimulation could activate the cutaneous sensory nerves to propagate impulses and release inflammatory neuropeptides CGRP and SP,
Evidence Sentence: (1) mast cells and blood vessels, (2) mast cells and SP-positive nerve fibers, (3) mast cells and CGRP-positive nerve fibers, (4) HA-expression on mast cells, and (5) 5-HT-expression on mast cells.
Evidence Sentence: We used a similar procedure in the double immunohistochemical staining to examine the correlation between mast cells and SP or CGRP-positive nerve fibers,
Evidence Sentence: Rabbit polyclonal anti-mast cell tryptase antibody and mouse monoclonal anti-CGRP antibody were employed to examine the correlation between mast cells and CGRP-positive nerve fibers.
Evidence Sentence: After MA stimulation, the SP and CGRP were distributed more around the local nerve fibers, and mast cells tended to gather around the SP- and CGRP-positive nerve fibers
Evidence Sentence: The correlation of mast cells and SP- or CGRP-positive nerve fibers in the area of LI4 were examined by double immunohistochemical staining (Fig. 3, Fig. 4).
Evidence Sentence: In this study we examined the distributions of SP- and CGRP-positive nerve fibers and morphological and chemical changes of mast cells in the area of acupoint LI4 after MA stimulation.
Evidence Sentence: (2) SP- and CGRP-positive nerve fibers responded to MA stimulation, displaying higher expression of SP and CGRP than those of the control, and, in turn, mast cells migrated toward SP- and CGRP-positive nerve fibers;
Evidence Sentence: In this study the higher expression of SP and CGRP on cutaneous nerve fibers in the area of LI4 suggests that the nerve fibers might be responding to MA stimulation.
Evidence Sentence: Therefore SP and CGRP mediates the neuronal responses of acupuncture.