Evidence Details for Calca
PMID Title Journal Year Abstract
29033603 Application of the chronic constriction injury of the partial sciatic nerve model to assess acupuncture analgesia. J Pain Res. 2017 Sep 19;10:2271-2280. doi: 10.2147/JPR.S139324. eCollection 2017. 2017 PURPOSE: To validate and explore the application of a rat model of chronic constriction injury to the partial sciatic nerve in investigation of acupuncture analgesia. METHODS: Chronic constriction injury of the sciatic nerve (CCI) and chronic constriction injury of the partial sciatic nerve (CCIp) models were generated by ligating either the sciatic nerve trunk or its branches in rats. Both models were evaluated via paw mechanical withdrawal latency (PMWL), paw mechanical withdrawal threshold (PMWT), nociceptive reflex-induced electromyogram (C-fiber reflex EMG), and dorsal root ganglion immunohistochemistry. Electroacupuncture (EA) was performed at GB30 to study the analgesic effects on neuropathic pain and the underlying mechanisms. RESULTS: Following ligation of the common peroneal and tibial nerves, CCIp rats exhibited hindlimb dysfunction, hind paw shrinkage and lameness, mirroring those of CCI rats (generated by ligating the sciatic nerve trunk). Compared to presurgery measurements, CCIp and CCI modeling significantly decreased the PMWL and PMWT. EA at GB30 increased the PMWL and PMWT in both CCI and CCIp rats. Calcitonin gene-related polypeptide and substance P expressions were apparently increased in both CCI and CCIp groups, but were not different from each other. The C-fiber reflex EMG of the biceps femoris was preserved in CCIp rats, but it could not be recorded in CCI rats on the 5th day after nerve ligation. The C-fiber reflex EMG was reduced at 0, 1, and 2 minutes after EA in CCIp rats, but only at 0 and 1 minute after EA in normal rats. CONCLUSION: The CCIp model is better than the CCI model for studying acupuncture analgesia on chronic neuropathic pain and the underlying mechanisms."

Evidence Sentence: Calcitonin gene-related polypeptide and substance P expressions were apparently increased in both CCI and CCIp groups, but were not different from each other.
Evidence Sentence: Expression of CGRP and SP in DRG
Evidence Sentence: Subsequently, we observed expression of CGRP and SP in DRG of L4-6 ipsilateral compared to contralateral both in CCI and CCIp models.
Evidence Sentence: As shown in Figure 4A and B, CGRP and SP expression in normal rats were not apparently different between CCI and CCIp groups.
Evidence Sentence: After modeling, CGRP and SP expression were increased in CCI and CCIp rats.
Evidence Sentence: These data suggest that ligation of the two branches of the sciatic nerve could increase the expression of CGRP and SP in a similar manner to the ligation of the trunk of the sciatic nerve.