Evidence Details for Calca
| PMID | Title | Journal | Year | Abstract |
|---|---|---|---|---|
| 37384285 | Attenuation of immobilization stress-induced hypertension by temperature-controllable warm needle acupuncture in rats and the peripheral neural mechanisms. | Front Neurol. 2023 Jun 13;14:1168012. doi: 10.3389/fneur.2023.1168012. eCollection 2023. | 2023 | INTRODUCTION: We and others have shown that electrical stimulation of the PC-6 acupoint over the wrist relieves hypertension by stimulating afferent sensory nerve fibers and activating the central endogenous opioid system. Warm needle acupuncture has long been utilized to treat various diseases in clinics. METHODS: Here, we developed a temperature-controllable warm needle acupuncture instrument (WAI) and investigated the peripheral mechanism underlying the effect of warm needle acupuncture at PC-6 on hypertension in a rat model of immobilization stress-induced hypertension. RESULTS: Stimulation with our newly developed WAI and traditional warm needle acupuncture attenuated hypertension development. Such effects were reproduced by capsaicin (a TRPV1 agonist) injection into PC-6 or WAI stimulation at 48 degrees C. In contrast, PC-6 pretreatment with the TRPV1 antagonist capsazepine blocked the antihypertensive effect of WAI stimulation at PC-6. WAI stimulation at PC-6 increased the number of dorsal root ganglia double-stained with TRPV1 and CGRP. QX-314 and capsaicin perineural injection into the median nerve for chemical ablation of small afferent nerve fibers (C-fibers) prevented the antihypertensive effect of WAI stimulation at PC-6. Additionally, PC-6 pretreatment with RTX ablated the antihypertensive effect of WAI stimulation. CONCLUSION: These findings suggest that warm needle acupuncture at PC-6 activates C-fiber of median nerve and the peripheral TRPV1 receptors to attenuate the development of immobilization stress-induced hypertension in rats." |
| Evidence Sentence: | WAI stimulation at PC-6 increased the number of dorsal root ganglia double-stained with TRPV1 and CGRP. |
| Evidence Sentence: | To explore which subtypes of TRPV1-expressing DRG neurons were activated by WAI stimulation at PC-6, double-staining of TRPV1 and the neuronal markers CGRP (a peptidergic small neuronal marker), IB4 (a nonpeptidergic small neuronal marker) or NF200 (a marker for sensory myelinated fibers) was performed. |
| Evidence Sentence: | The fluorescence intensity of TRPV1-immunoreactive neurons colocalized with CGRP was significantly higher in the WAI-stimulated group (n = 6) than in the normal group (Figures 5A,B) (t-test, F = 3.164, p < 0.001), and the number of TRPV1 neurons double-labeled with IB4 was significantly higher in the WAI group (n = 6) than in the normal group (Figures 5E,F) (n = 6, t-test, F = 1.249, p < 0.05). |
| Evidence Sentence: | To investigate whether the nerve fibers of the skin were chemically ablated by RTX, we double-stained the nerve fibers with anti-PGP 9.5 antibody (a marker for intraepidermal general nerve fibers) and anti-CGRP antibody (a marker for peripheral peptidergic nerve fibers). |
| Evidence Sentence: | The injection of RTX into PC-6 significantly decreased the fluorescence intensity of PGP9.5/CGRP compared to vehicle treatment (one-way ANOVA; F = 61.12, p < 0.0001; Figures 6D,E). |