| Evidence Sentence: |
The purpose of this study is to investigate whether eotaxin/CC chemokine receptor 3 (CCR3) axis, a key regulatory pathway for eosinophils (EOS) recruitment, is involved in acupuncture analgesia for dysmenorrhea. |
| Evidence Sentence: |
Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation |
| Evidence Sentence: |
The purpose of this study is to investigate whether eotaxin/CC chemokine receptor 3 (CCR3) axis, a key regulatory pathway for eosinophils (EOS) recruitment, is involved in acupuncture analgesia for dysmenorrhea. |
| Evidence Sentence: |
The expression of CCR3 and histamine H1 receptor (H1R) was analyzed by RT-qPCR and Western blot. |
| Evidence Sentence: |
In addition, TN decreased the release of eotaxin, HIS, IL-6, and the expression of CCR3 and H1R. |
| Evidence Sentence: |
TN alleviated menstrual pain by improving the uterine inflammatory environment via suppressing eotaxin/CCR3 axis to weak EOS-MC activation in CCD rats. |
| Evidence Sentence: |
TN Reduced Eotaxin Levels and Attenuated the Expression of CCR3 in the Uterus |
| Evidence Sentence: |
To determine whether TN could modulate eotaxin/CCR3 axis, we applied ELISA to detect the eotaxin level, RT-qPCR, and Western blot to analyze the expression of CCR3. |
| Evidence Sentence: |
This attenuation was accompanied by decreased mRNA and protein expression of CCR3 (P < 0.01; Figures 4(g) and 4(j)). |
| Evidence Sentence: |
To investigate whether the HIS expression in uterus is associated with the eotaxin/CCR3 axis, we applied ELISA to detect the HIS level, RT-qPCR, and Western blot to analyze the expression of its receptor, H1R. |
| Evidence Sentence: |
To observe the association between eotaxin/CCR3 axis and HIS, we applied HE or TB, respectively, to analyze the main inflammatory cells, EOS and MC. |
| Evidence Sentence: |
TN at SP6 can relieve pain by attenuating the expression of eotaxin/CCR3 axis and EOS-MC activation. |
| Evidence Sentence: |
To further address whether pain-relief induced by TN was dependent on the activation of the eotaxin/CCR3 axis, we injected CCR3 antagonist SB328437 and agonist CCL11. |
| Evidence Sentence: |
After administration of SB328437, compared to the model group, the ECP expression and the HIS level were significantly decreased in the Model + SB328437 group (P < 0.01; Figures 8(a)-8(c)), which indicated that the antagonist of CCR3 could mimic the effects of TN. |
| Evidence Sentence: |
The CCR3 agonist CCL11 was administered 30 min prior to acupuncture. |
| Evidence Sentence: |
CCR3 Agonist Blocked TN-Induced Analgesic Effect |
| Evidence Sentence: |
CCR3 Agonist Reversed the Decreased ECP Expression and HIS Level in the Uterus Induced by TN |
| Evidence Sentence: |
To further address whether pain-relief induced by TN was dependent on the activation of the eotaxin/CCR3 axis, we injected CCR3 antagonist SB328437 and agonist CCL11. |
