Evidence Details for Cdk4
PMID Title Journal Year Abstract
23837037 Electroacupuncture at Quchi and Zusanli treats cerebral ischemia-reperfusion injury through activation of ERK signaling. Exp Ther Med. 2013 Jun;5(6):1593-1597. doi: 10.3892/etm.2013.1030. Epub 2013 Mar 26. 2013 Jun The extracellular signal-regulated kinase (ERK) pathway, a critical mediator of cell proliferation, is activated in cerebral ischemia/reperfusion (I/R) injury and is therefore a key target in the treatment of ischemic stroke. Acupuncture has long been used in China to clinically treat stroke. However, the precise mechanism of its neuroprotective activities remains largely unknown. In the present study, a focal cerebral I/R-injured rat model was used to evaluate the in vivo therapeutic efficacy of electroacupuncture (EA) and investigate the underlying molecular mechanisms. EA significantly ameliorated neurological deficits and cerebral infarction in cerebral I/R-injured rats. Moreover, EA significantly increased the phosphorylation levels of ERK, as well as the protein expression levels of Ras, cyclin D1 and cyclin-dependent kinase (CDK)4. Consequently, EA-mediated activation of the ERK pathway resulted in the stimulation of cerebral cell proliferation. The present data suggest that EA at the Quchi and Zusanli acupoints exerts a neuroprotective effect in ischemic stroke via the activation of ERK signaling."

Evidence Sentence: Moreover, EA significantly increased the phosphorylation levels of ERK, as well as the protein expression levels of Ras, cyclin D1 and cyclin-dependent kinase (CDK)4.
Evidence Sentence: Electroacupuncture at Quchi and Zusanli increases the expression of cyclin D1 and CDK4 in cerebral I/R-injured rats
Evidence Sentence: To further investigate the mechanism of the pro-proliferative activity of EA, its effect on the protein expression of cyclin D1 and CDK4 was evaluated using IHC staining (Figs.
Evidence Sentence: Consistent with the previous findings, the protein expression levels of cyclin D1 and CDK4 in the ischemic cerebral cortex and striatum were increased by I/R injury and further upregulated by EA treatment.