Evidence Details for Crhr1
PMID Title Journal Year Abstract
37435647 Electroacupuncture in the treatment of IBS in rats: investigation of the mechanisms of CRH(+) neurons in the paraventricular nucleus. J Neurophysiol. 2023 Aug 1;130(2):380-391. doi: 10.1152/jn.00156.2023. Epub 2023 Jul 12. 2023 Aug 1 Electroacupuncture (EA) is well documented to treat irritable bowel syndrome (IBS). However, the mechanism of the central nervous system related to IBS and acupuncture stimulation is still not well known. In this study, a rat model of IBS was established by cold-restraint comprehensive stresses for 15 days, and it was found that the levels of corticotropin-releasing hormone (CRH), corticosterone (CORT), and adrenocorticotropic hormone (ACTH) in the peripheral serum were increased; the visceral sensitivity was enhanced; and the intestinal motility was accelerated, specifically, there was an enhancement in the discharge frequency of neurons in the paraventricular nucleus (PVN). EA treatment for 3 days, 20 min/day, alleviated the increase in the levels of CRH, CORT, and ACTH in the peripheral serum of rats, reduced the visceral sensitivity of IBS rats, and inhibited colon movement and discharge frequency of the neurons in the PVN. In addition, EA could reduce the excitability of CRH neurons and the expression of corticotropin-releasing hormone receptor 1 (CRHR1) and corticotropin-releasing hormone receptor 2 (CRHR2) in PVN. At the same time, the expression of CRH, CRHR1, and CRHR2 in the peripheral colon was decreased. Taken together, EA appears to regulate intestinal functional activity through the central CRH nervous system, revealing the central regulation mechanism of EA in IBS rats, and providing a scientific research basis for the correlation among the meridians, viscera, and brain.NEW & NOTEWORTHY The purpose of this research was to determine the central regulatory mechanism of electroacupuncture (EA) in rats with irritable bowel syndrome (IBS). Our results showed that combined with the serum changes in corticotropin-releasing hormone (CRH), corticosterone (CORT), and adrenocorticotropic hormone (ACTH), the improvement of IBS by EA was related to them. Furthermore, EA could regulate intestinal functional activity through the central CRH(+) nervous system."

Evidence Sentence: EA Affected Intestinal Functional Activity by Reducing the Expression of CRH, CRHR1, and CRHR2 in the Colon
Evidence Sentence: In addition, EA could reduce the excitability of CRH neurons and the expression of corticotropin-releasing hormone receptor 1 (CRHR1) and corticotropin-releasing hormone receptor 2 (CRHR2) in PVN.
Evidence Sentence: At the same time, the expression of CRH, CRHR1, and CRHR2 in the peripheral colon was decreased.
Evidence Sentence: EA Affected Intestinal Functional Activity by Regulating the Expression of CRHR1 and CRHR2 in the PVN of Experimental Rats
Evidence Sentence: This study demonstrated whether EA could regulate the expression of CRHR1 and CRHR2 in PVN (Fig.
Evidence Sentence: Compared with the hm3D(Gq) group, the EA + hm3D(Gq) group showed significantly decreased expression of CRHR1 [P < 0.05, F(4,20) = 41.60] and CRHR2 [P < 0.05, F(4,20) = 34.51] in the PVN; there was a significant difference between the hm4D(Gi) group and the EA + hm4D(Gi) group.
Evidence Sentence: It was confirmed that EA could regulate the expression of CRHR1 and CRHR2 in the PVN of IBS rats.
Evidence Sentence: Compared with the EA group, the EA + hm3D(Gq) group showed significantly increased expression of CRHR1 and CRHR2 in the PVN; there was a significant difference between the EA group and the EA + hm4D(Gi) group.
Evidence Sentence: Next, we determined whether EA could regulate the expressions of CRH, CRHR1, and CRHR2 in the colon (Fig.
Evidence Sentence: Compared with the hm3D(Gq) group, the EA + hm3D(Gq) group showed significantly decreased expression of CRH [P < 0.05, F(4,20) = 5], CRHR1 [P < 0.05, F(4,20) = 87.47], and CRHR2 [P < 0.05, F(4,20) = 34.35] in the colon; there was a significant difference between the hm4D(Gi) group and the EA + hm4D(Gi) group.
Evidence Sentence: This confirmed that EA could regulate the expression of CRH, CRHR1, and CRHR2 in the colon of IBS rats.
Evidence Sentence: Compared with the EA group, the EA + hm3D(Gq) group showed significantly increased expression of CRH, CRHR1, and CRHR2 in the colon, there was a significant difference between the EA group and the EA + hm4D(Gi) group.