Evidence Details for FOXO1
PMID Title Journal Year Abstract
25695055 Acupuncture may exert its therapeutic effect through microRNA-339/Sirt2/NFkappaB/FOXO1 axis. Biomed Res Int. 2015;2015:249013. doi: 10.1155/2015/249013. Epub 2015 Jan 28. 2015 Recently, we have found that a number of microRNAs (miRNAs) and proteins are involved in the response to acupuncture therapy in hypertensive rats. Our bioinformatics study suggests an association between these miRNAs and proteins, which include miR-339 and sirtuin 2 (Sirt2). In this paper, we aimed to investigate whether Sirt2 was a direct target of miR-339 in neurons. In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339. Overexpression of miR-339 downregulated Sirt2 expression, while knockdown of miR-339 upregulated Sirt2 expression in human SH-SY5Y cells and rat PC12 cells. In addition, overexpression of miR-399 increased the acetylation of nuclear factor-kappaB (NF-kappaB) and forkhead box protein O1 (FOXO1) in SH-SY5Y cells, which are known targets of Sirt2. Our findings demonstrate that miR-339 regulates Sirt2 in human and rat neurons. Since Sirt2 plays a critical role in multiple important cellular functions, our data imply that acupuncture may act through epigenetic changes and subsequent action on their targets, such as miRNA-339/Sirt2/NF-kappaB/FOXO1 axis. Some physiological level changes of neurons after altering the miR-339 levels are needed to validate the suggested therapeutic role of miR-339/Sirt2/NF-kappaB/FOXO1 axis in response to acupuncture therapy in the future work."

Evidence Sentence: Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339/Sirt2/NFkappaB/FOXO1 Axis
Evidence Sentence: In addition, overexpression of miR-399 increased the acetylation of nuclear factor-kappaB (NF-kappaB) and forkhead box protein O1 (FOXO1) in SH-SY5Y cells, which are known targets of Sirt2.
Evidence Sentence: Since Sirt2 plays a critical role in multiple important cellular functions, our data imply that acupuncture may act through epigenetic changes and subsequent action on their targets, such as miRNA-339/Sirt2/NF-kappaB/FOXO1 axis.
Evidence Sentence: Some physiological level changes of neurons after altering the miR-339 levels are needed to validate the suggested therapeutic role of miR-339/Sirt2/NF-kappaB/FOXO1 axis in response to acupuncture therapy in the future work.
Evidence Sentence: Overexpression of miRNA-339 Upregulates the Acetylated NF-kappaB and FOXO1 Expression Level in SH-SY5Y Cells
Evidence Sentence: Our results showed that Sirt2 is the direct target of miR-339, indicating that miR-339 can contribute to an upregulation of the acetylated status of Sirt2 target, including NF-kappaB and FOXO1.
Evidence Sentence: To test this hypothesis, we measured the acetylated status of Sirt2 target (NF-kappaB and FOXO1) in SH-SY5Y cells treated with miRNA-339 mimic.
Evidence Sentence: As we expected, overexpression of miRNA-339 significantly increased the acetylation level of NF-kappaB and FOXO1 in a time-dependent manner (Figures 5(a) and 5(b), P < 0.01 versus control).