| Evidence Sentence: |
Effects of electroacupuncture on the expression of hypothalamic neuropeptide Y and ghrelin in pubertal rats with polycystic ovary syndrome |
| Evidence Sentence: |
But, surprisingly, SA seem to make no difference to the levels of FBG and insulin, and the protein expression of ghrelin in the hypothalamus and ARC. |
| Evidence Sentence: |
EA and SA improved the symptoms of pubertal PCOS rats, and the mechanism might be associated with regulating hypothalamic NPY and ghrelin levels. |
| Evidence Sentence: |
The ghrelin level decreased in model rats (p<0.01) and could be rescued by treatment with either EA or SA (p<0.05) (Fig 3E). |
| Evidence Sentence: |
The expression of ghrelin protein (Fig 4A) and mRNA (Fig 4F) in the hypothalamus significantly decreased in the model group (both p<0.01) and EA reversed their expression (both p<0.01), while SA only reversed the mRNA expression (both p<0.01). |
| Evidence Sentence: |
The serum levels of insulin, neuropeptide Y(NPY) and fasting blood glucose(FBG) increased significantly (both p < 0.01), while the serum levels of ghrelin(GHRL) decreased in the model group (p < 0.01). |
| Evidence Sentence: |
After treatment with EA, the levels of NPY (p < 0.01) and FBG (p < 0.05) went into decrease, whereas the levels of GHRL (p < 0.05) and insulin (p < 0.01) increased. |
| Evidence Sentence: |
The upregulation of NPY mRNA and neuropeptide Y2 receptor(NPY2R) mRNA and the downregulation of GHRL protein and mRNA in the hypothalamus, and the increased expression of NPY and NPY2R as well as the decreased expression of GHRL in the arcuate nucleus (ARC) can be rescued by EA. |
| Evidence Sentence: |
No differences were found between groups in protein of GAL, GALP and GHRL expression in the pancreas. |
| Evidence Sentence: |
Expression of GAL, GALP, GHRL and NPY in the hypothalamus |
| Evidence Sentence: |
Expression of GHRL, NPY, NPY2R and GHSR in the arcuate nucleus (ARC) |
| Evidence Sentence: |
According to the RT-PCR and WB results, we found that there were differences in the expression of NPY and GHRL in the hypothalamus. |
| Evidence Sentence: |
Given that the arcuate nucleus (ARC) is a key site of the reproductive regulatory system and the energy regulating system, therefore, we performed an immunohistochemistry test to determine the expression of NPY, GHRL and their receptor in the ARC. |
| Evidence Sentence: |
Meanwhile, the number of GHRL-positive cells in the ARC in group M was much lower than the number in groups N and EA (P < 0.01, P < 0.05, respectively), but there was no difference between groups M and SA (Fig 5).There was some attenuated immunoreactivity of NPY2R in the ARC in group M compared with group N (P < 0.05), but when treated with EA or SA, the immunoreactivity of NPY2R was enhanced remarkably (P < 0.01, P < 0.05, respectively) (Fig 5). |
| Evidence Sentence: |
Protein expression of kisspeptin, GALP, GAL and GHRL in the pancreas |
| Evidence Sentence: |
The protein expression of GALP, GAL and GHRL did not differ between the four groups (Fig 7B-7D). |
