Evidence Details for Gsk3b
PMID Title Journal Year Abstract
25120577 A Study on the Effect of Neurogenesis and Regulation of GSK3beta/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats. Evid Based Complement Alternat Med. 2014;2014:962343. doi: 10.1155/2014/962343. Epub 2014 Jul 10. 2014 170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's ""self-healing"" capacity 3 days after MCAO. And the ""self-healing"" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3beta and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3beta and PP2A expression in MCAO rats."

Evidence Sentence: A Study on the Effect of Neurogenesis and Regulation of GSK3beta/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats
Evidence Sentence: And the self-healing capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3beta and PP2A expression.
Evidence Sentence: In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3beta and PP2A expression in MCAO rats.
Evidence Sentence: Acupuncture Made a Positive Regulation on GSK-3beta, PP2A, and G/P
Evidence Sentence: Thus, to verify the microarray analysis results, we evaluated GSK-3beta and PP2A expression (which well-accepted have closely connection of biological processes mentioned above) in different cerebral regions of rats by ELISA.
Evidence Sentence: As demonstrated in Figure 7(a), GSK-3beta expression robust increased in MCAO groups, whereas PP2A expression conspicuous decreased.
Evidence Sentence: And expression of GSK-3beta in no-acupuncture was decreased meanwhile increased of PP2A expression compared to model group (P < 0.05).
Evidence Sentence: However, acupuncture shown a regulation effects on expression tendency of GSK-3beta and PP2A: it enhanced the increasing of GSK-3beta while attenuated decreasing of PP2A (P < 0.05).
Evidence Sentence: And the similar tendency of GSK-3beta and PP2A expression was found in cortex, hippocampus and striatum.
Evidence Sentence: The ratio of GSK-3beta and PP2A was also dramatically elevated post-ischemia, but decreased in no-acupuncture group and acupuncture group (P < 0.05).
Evidence Sentence: The results revealed that it was a temporal profile of GSK-3beta and PP2A expression, and the ratio of GSK-3beta and PP2A (G/P) positive regulated by acupuncture stimulation.