Evidence Details for Htr1a
| PMID | Title | Journal | Year | Abstract |
|---|---|---|---|---|
| 27301303 | In vivo Monitoring of Serotonin by Nanomaterial Functionalized Acupuncture Needle. | Sci Rep. 2016 Jun 15;6:28018. doi: 10.1038/srep28018. | 2016 Jun 15 | Acupuncture treatment is amazing but controversial. Up to now, the mechanism of treating diseases by acupuncture and moxibustion is still unclear, especially the occurrence of the molecular events in local acupoints. Herein, we report an extremely stable microsensor by modifying carbon nanotube (CNT) to the tip surface of acupuncture needle and applying this CNT-modified acupuncture needle for real time monitoring of serotonin (5-HT) in vivo. To stabilize CNT modification on the needle tip surface, poly(3,4-ethylenedioxythiophene)(PEDOT) was employed as glue water to stick CNT on the needle. The detection limit of the CNT-modified needle was found to be approximately 50 nM and 78 nM in the PBS and the cell medium, respectively. In addition, the needle showed good selectivity to some inflammatory mediators and some electroactive molecules. For the first time, the CNT-modified needle could be directly probed into rat body for real time monitoring of 5-HT in vivo, showing a great potential for better understanding the mechanism of acupuncture treatment." |
| Evidence Sentence: | Herein, we report an extremely stable microsensor by modifying carbon nanotube (CNT) to the tip surface of acupuncture needle and applying this CNT-modified acupuncture needle for real time monitoring of serotonin (5-HT) in vivo. |
| Evidence Sentence: | For the first time, the CNT-modified needle could be directly probed into rat body for real time monitoring of 5-HT in vivo, showing a great potential for better understanding the mechanism of acupuncture treatment. |
| Evidence Sentence: | Mast cells are known as immune cells that play an active role in the immune response through degranulation to release biological substances including tryptase, histamine, and serotonin (5-HT) et al. |
| Evidence Sentence: | Serotonin (5-HT), as a representative substance released by mast cells, plays a pivotal role in alleviating pain. |
| Evidence Sentence: | So far, 5-HT levels in the central nervous and in the blood of platelets have been assayed by extraction, followed by high precision liquid chromatography (HPLC) analysis14 and enzyme-linked immunosorbent assay (ELISA) |
| Evidence Sentence: | As illustrated in Fig. 1, CNT and PEDOT are assembled on the tip surface of the acupuncture needle, and 5-HT can be monitored in local acupionts in vivo by using the modified acupuncture needle. |
| Evidence Sentence: | Results show that PEDOT and CNT have a synergistic effect towards the electrocatalytic reaction of 5-HT. |