| Evidence Sentence: |
The protein expression of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta were also detected by Western blot. |
| Evidence Sentence: |
The expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-alpha, IL-1beta and miR-449a was compared. |
| Evidence Sentence: |
At day 7, compared with sham-operated group, miR-449a expression in SCI group was significantly increased (P < 0.05), and NeuN and CGRP mRNA and protein expression was markedly decreased (P < 0.05), but protein levels of Nestin, cleaved caspase 3, TNF-alpha, IL-1beta and the ratio of Bax/Bcl-2 in SCI group were significantly increased (P < 0.05). |
| Evidence Sentence: |
The EA treatment significantly reduced miR-449a level and cleaved caspase 3, TNF-alpha, IL-1beta level and the ratio of Bax/Bcl-2 (P < 0.01), but substantially increased Nestin, NeuN and CGRP expression (P < 0.05 or 0.01). |
| Evidence Sentence: |
High level of miR-449a in miR subgroup was accompanied by decreased expression of Nestin, NeuN and CGRP and increased expression of cleaved caspase 3, TNF-alpha, IL-1beta and elevation of the ratio of Bax/Bcl-2 (P < 0.05), suggesting miR-449a inhibits the effects of EA on NSCs and neurons. |
| Evidence Sentence: |
EA inhibit the SCI-induced increase of cleaved caspase 3, Bax/Bcl-2 ratio, TNF-alpha and IL-1beta in spinal cord of rats |
| Evidence Sentence: |
The protein levels of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta in ventral horn of spinal cord of rats were detected using Western blot and the results were shown in Figure 2(Fig. |
| Evidence Sentence: |
Densitometric analysis showed that relative protein level of cleaved caspase 3, TNF-alpha and IL-1beta and the ratio of Bax to Bcl-2 were all significantly elevated in SCI group than that in sham-operated group (P < 0.05 or 0.01). |
| Evidence Sentence: |
These results indicate that EA could suppress the SCI-stimulated elevation of cleaved caspase 3, TNF-alpha and IL-1beta, and Bax/Bcl-2 ratio; EA might inhibit cell apoptosis and inflammation in ventral horn of injured spinal cord of rats. |
| Evidence Sentence: |
Effect of miR-449a agomir on EA-stimulated Nestin, NeuN and CGRP expression and EA-reduced level of cleaved caspase 3, TNF-alpha and IL-1beta and the ratio of Bax to Bcl-2 |
| Evidence Sentence: |
In addition, protein expression detection of cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta showed that the relative expression of cleaved caspase 3, TNF-alpha and IL-1beta proteins and Bax/Bcl-2 ratio in miR group were obviously higher than that in EA group (P < 0.05 or 0.01) (Figure 3I-L(Fig. |
| Evidence Sentence: |
These results suggest that high level of miR-449a could suppress the effect of EA on the expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-alpha and IL-1beta in spinal cord of rats. |
