Evidence Details for Irak1
PMID Title Journal Year Abstract
26339273 Herb-Partitioned Moxibustion Regulates the TLR2/NF-kappaB Signaling Pathway in a Rat Model of Ulcerative Colitis. Evid Based Complement Alternat Med. 2015;2015:949065. doi: 10.1155/2015/949065. Epub 2015 Aug 3. 2015 The TLR2/NF-kappaB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-kappaB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-kappaB was significantly increased and IFN-beta and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-beta and upregulating the expression of IFN-beta and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa."

Evidence Sentence: The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats.
Evidence Sentence: Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats.
Evidence Sentence: IRAK1 mRNA and Protein Expression in Rat Colonic Mucosa
Evidence Sentence: As shown in Figure 6, rats in the UC group expressed significantly higher levels of IRAK1 mRNA in the colonic mucosa than rats in the control group (P < 0.01).
Evidence Sentence: Between the two treatment groups, IRAK1 mRNA expression in the colonic mucosa was significantly higher with SASP than with herb-partitioned moxibustion treatment (P < 0.01).
Evidence Sentence: As shown in Figures 7 and 8, IRAK1 protein levels in the colonic mucosa were significantly higher in UC rats than in the control rats, as measured by both the total area of staining and the integrated optical density (P < 0.01 in both cases).
Evidence Sentence: Both herb-partitioned moxibustion and SASP treatments significantly reduced IRAK1 protein levels in the colonic mucosa of UC rats, as indicated by both of these measurements (P < 0.01 in all cases).
Evidence Sentence: IRAK1 protein levels in the colonic mucosa were significantly lower in the herb-partitioned moxibustion than in the SASP treatment by both of these measurements (P < 0.01 in both cases).
Evidence Sentence: Both herb-partitioned moxibustion and SASP treatments significantly reduced IRAK-1 mRNA expression in the colonic mucosa of UC rats (P < 0.01, P < 0.05).