| Evidence Sentence: |
Inversely, upregulating miR-34a-5p expression inhibited microglia M2 polarization while accelerated M1 polarization through targeting Kruppel-like factor 4 (Klf4), and thereby diminished the protective effect of DU20-penetrating-GB7 acupuncture on ICH. |
| Evidence Sentence: |
Effects of miR-34a-5p on Klf4 expression in the perihemorrhagic penumbra of ICH rats with Baihui-penetrating-Qubin acupuncture treatment |
| Evidence Sentence: |
Contrary to the trend of miR-34a-5p expression, the mRNA and protein levels of Klf4 were decreased with miR-34a-5p overexpression (Figs. |
| Evidence Sentence: |
2B and C, P<0.05), suggesting Klf4 might be one of the targets of miR-34a-5p. |
| Evidence Sentence: |
Targeted binding between miR-34a-5p and Klf4 3'-UTR |
| Evidence Sentence: |
To study the underlying molecular mechanism between miR-34a-5p and Klf4, Dual-Luciferase reporter assay was carried out. |
| Evidence Sentence: |
5A, relative firefly luciferase activity was obviously reduced in co-expressed miR-34a-5p and wild-type Klf4 3'-UTR (P<0.05). |
| Evidence Sentence: |
The results confirmed the hypothesis that miR-34a-5p negatively regulated Klf4 level via targeted binding Klf4 3'-UTR. |
| Evidence Sentence: |
Baihui (DU20)-penetrating-Qubin (GB7) acupuncture regulates microglia polarization through miR-34a-5p/Klf4 signaling in intracerebral hemorrhage rats |
| Evidence Sentence: |
The results suggested the therapeutic effect of DU20-penetrating-GB7 acupuncture on ICH might be attributed to its modulation on microglia polarization through miR-34a-5p/Klf4 signaling. |
