Evidence Details for Lamp1
PMID Title Journal Year Abstract
26991319 Anti-Inflammatory Effects of Acupuncture Stimulation via the Vagus Nerve. PLoS One. 2016 Mar 18;11(3):e0151882. doi: 10.1371/journal.pone.0151882. eCollection 2016. 2016 Although acupuncture therapy is widely used in traditional Asian medicine for the treatment of diverse internal organ disorders, its underlying biological mechanisms are largely unknown. Here, we investigated the functional involvement of acupuncture stimulation (AS) in the regulation of inflammatory responses. TNF-alpha production in mouse serum, which was induced by lipopolysaccharide (LPS) administration, was decreased by manual acupuncture (MAC) at the zusanli acupoint (stomach36, ST36). In the spleen, TNF-alpha mRNA and protein levels were also downregulated by MAC and were recovered by using a splenic neurectomy and a vagotomy. c-Fos, which was induced in the nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) by LPS and electroacupuncture (EAC), was further increased by focal administration of the AMPA receptor blocker CNQX and the purinergic receptor antagonist PPADS. TNF-alpha levels in the spleen were decreased by CNQX and PPADS treatments, implying the involvement of inhibitory neuronal activity in the DVC. In unanesthetized animals, both MAC and EAC generated c-Fos induction in the DVC neurons. However, MAC, but not EAC, was effective in decreasing splenic TNF-alpha production. These results suggest that the therapeutic effects of acupuncture may be mediated through vagal modulation of inflammatory responses in internal organs."

Evidence Sentence: In ALP, microtubule-associated protein 1 light chain 3 II (LC3II) is a typical protein marker associated with the completed autophagosomes, and lysosome associated membrane protein 1 (LAMP1) is a lysosomal structural protein.
Evidence Sentence: We first examined the expression levels of LC3II and LAMP1 in SNpc of PG mice.
Evidence Sentence: We found that LC3II was up-regulated, suggesting either the induction of autophagy or interruption of the fusion of autophagosome and lysosome, and LAMP1 was down-regulated, suggesting the impairment of lysosome (Fig.
Evidence Sentence: Significantly, compared with mice in PG, we observed ~37% decrease in the expression level of LC3II and ~20% in LAMP1 on the 4th day in AG, along with over 50% clearance of alpha-syn in SNpc (Fig.
Evidence Sentence: Since LC3II and LAMP1 are both downstream ALP proteins, we further examined the acupuncture effects on the expression of upstream ALP proteins of p-mTOR, p-P70S6K and ULK1 (Fig.
Evidence Sentence: The effects of rapamycin on downstream proteins (LC3II and LAMP1) in the rapamycin-treated group (RG) were generally similar to those of acupuncture (Fig.