Evidence Details for Lep
PMID Title Journal Year Abstract
28806763 Genome-wide regulation of electro-acupuncture on the neural Stat5-loss-induced obese mice. PLoS One. 2017 Aug 14;12(8):e0181948. doi: 10.1371/journal.pone.0181948. eCollection 2017. 2017 Acupuncture is reported to be effective in treating obesity related illnesses, but its mechanism is still unclear. To investigate this mechanism we applied electro-acupuncture (EA) in a mouse model of obesity and used RNA-seq to identify molecular consequences. Deletion of the transcription factor STAT5 from neurons (Stat5NKO) led to obesity. Acupuncture, in turn, reduced body weight and the ratio of epididymal white adipose tissue (Epi-WAT) to body weight, and it also decreased plasma concentrations of glucose, triglyceride, and cholesterol. In addition, EA increased cold endurance of Stat5NKO obese mice. EA reversed altered gene expressions in the hypothalamus and Epi-WAT, especially in the hypothalamus in Stat5NKO obese mice. This study provides, for the first time, insight into genomic networks of obesity and their modulation by electro-acupuncture, which in turn reveals potential mechanisms that explain acupuncture-induced weight-loss."

Evidence Sentence: Moreover, based on the top 50 DEG list, 41 common genes whose expression levels were up-regulated in the Stat5NKO group were dramatically decreased after EA treatment, and most of these genes were closely related to the development of obesity, such as Gh, Prl, Lep, Igfals, Plin1, Agpat2, Mogat2, and Cidec (S6 Table).
Evidence Sentence: Although Lep (the gene encoding Leptin) was not included in the top 50 genes down-regulated by EA in the hypothalamus, its FPKM value of the Stat5 fl/fl mice was 4.33, but was decreased from 58.59 to 4.66 in the Stat5NKO+EA group.
Evidence Sentence: We found that 21 known genes appeared on the list for the hypothalamus of Stat5NKO mice (Fig 5A), and two of them (Prl, Lep) were up-regulated in the hypothalamus of the Stat5NKO group and were decreased by EA, but only one gene (Etv5) was down-regulated due to the loss of Stat5.
Evidence Sentence: Serum leptin and glucose levels were increased after the deletion of neural Stat5 gene (P<0.01) but were restored to baseline levels similar to those in the Stat5fl/fl mice following EA treatment (Fig 1D).
Evidence Sentence: Although Lep (the gene encoding Leptin) was not included in the top 50 genes down-regulated by EA in the hypothalamus, its FPKM value of the Stat5 fl/fl mice was 4.33, but was decreased from 58.59 to 4.66 in the Stat5NKO+EA group.