Evidence Details for Mapk1
PMID Title Journal Year Abstract
37383281 Acupuncture at Back-Shu point improves insomnia by reducing inflammation and inhibiting the ERK/NF-kappaB signaling pathway. World J Psychiatry. 2023 Jun 19;13(6):340-350. doi: 10.5498/wjp.v13.i6.340. eCollection 2023 Jun 19. 2023 Jun 19 BACKGROUND: Insomnia is a disease where individuals cannot maintain a steady and stable sleep state or fail to fall asleep. Western medicine mainly uses sedatives and hypnotic drugs to treat insomnia, and long-term use is prone to drug resistance and other adverse reactions. Acupuncture has a good curative effect and unique advantages in the treatment of insomnia. AIM: To explore the molecular mechanism of acupuncture at Back-Shu point for the treatment of insomnia. METHODS: We first prepared a rat model of insomnia, and then carried out acupuncture for 7 consecutive days. After treatment, the sleep time and general behavior of the rats were determined. The Morris water maze test was used to assess the learning ability and spatial memory ability of the rats. The expression levels of inflammatory cytokines in serum and the hippocampus were detected by ELISA. qRT-PCR was used to detect the mRNA expression changes in the ERK/NF-kappaB signaling pathway. Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB. RESULTS: Acupuncture can prolong sleep duration, and improve mental state, activity, diet volume, learning ability and spatial memory. In addition, acupuncture increased the release of 1L-1beta, 1L-6 and TNF-alpha in serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-kappaB signaling pathway. CONCLUSION: These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-kappaB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippo-campus."

Evidence Sentence: Acupuncture at Back-Shu point improves insomnia by reducing inflammation and inhibiting the ERK/NF-kappaB signaling pathway
Evidence Sentence: qRT-PCR was used to detect the mRNA expression changes in the ERK/NF-kappaB signaling pathway.
Evidence Sentence: In addition, acupuncture increased the release of 1L-1beta, 1L-6 and TNF-alpha in serum and the hippocampus and inhibited the mRNA and protein expression of the ERK/NF-kappaB signaling pathway.
Evidence Sentence: These findings suggest that acupuncture at Back-Shu point can inhibit the ERK/NF-kappaB signaling pathway and treat insomnia by increasing the release of inflammatory cytokines in the hippo-campus.
Evidence Sentence: Acupuncture at Back-Shu point inhibited the ERK/NF-kappaB signaling pathway
Evidence Sentence: The ERK/NF-kappaB signaling pathway is closely related to the body's immune system.
Evidence Sentence: The protein expression levels of the ERK/NF-kappaB signaling pathway related genes were detected by Western blot and immunohistochemistry, and the results were consistent with the trend in mRNA expression (Figure 5).
Evidence Sentence: In conclusion, we believe that acupuncture at Back-Shu point can improve insomnia in rats by inhibiting the ERK/NF-kappaB signaling pathway.
Evidence Sentence: Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB.
Evidence Sentence: U0126 is a highly potent inhibitor of ERK1/2, MEK-1, and MEK-2.
Evidence Sentence: The qRT-PCR results showed that compared with the Con group, the mRNA expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB in the hippocampus of the Model group were increased.