Evidence Details for Mapk3
PMID Title Journal Year Abstract
23970928 Moxibustion Inhibits the ERK Signaling Pathway and Intestinal Fibrosis in Rats with Crohn's Disease. Evid Based Complement Alternat Med. 2013;2013:198282. doi: 10.1155/2013/198282. Epub 2013 Jul 22. 2013 Intestinal fibrosis is the main pathological process in Crohn's disease (CD); acupuncture and moxibustion can inhibit the process of fibrosis in CD rats, but the regulatory mechanism remains unknown. The present study observed the effect of moxibustion on the extracellular signal-regulated kinase (ERK) signaling pathway in the CD rat. The result shows that the phosphorylation of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and the expression of the corresponding mRNAs in the colon tissue of CD rat were significantly higher than the normal control group. Both treatments with mild moxibustion and with herb-separated moxibustion significantly reduced the expression of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and Ras and Raf-1 mRNA. MEK-1 and ERK-1/2 mRNA expression in each treatment group showed a downward trend, and the ERK-1/2 mRNA levels were significantly lower in the mild moxibustion group. It indicates that Ras, Raf-1, MEK-1, and ERK-1/2 are involved in the process of intestinal fibrosis in CD rats. Moxibustion can downregulate the abnormal expression of colonic Ras, Raf-1, MEK-1, and ERK-1/2 protein and mRNA levels in CD intestinal fibrosis in rats. Moxibustion may play a role in the treatment of CD intestinal fibrosis by regulating ERK signaling pathway."

Evidence Sentence: Moxibustion Inhibits the ERK Signaling Pathway and Intestinal Fibrosis in Rats with Crohn's Disease
Evidence Sentence: The present study observed the effect of moxibustion on the extracellular signal-regulated kinase (ERK) signaling pathway in the CD rat.
Evidence Sentence: Moxibustion may play a role in the treatment of CD intestinal fibrosis by regulating ERK signaling pathway.
Evidence Sentence: The result shows that the phosphorylation of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and the expression of the corresponding mRNAs in the colon tissue of CD rat were significantly higher than the normal control group.
Evidence Sentence: Both treatments with mild moxibustion and with herb-separated moxibustion significantly reduced the expression of the Ras, Raf-1, MEK-1, and ERK-1/2 proteins and Ras and Raf-1 mRNA.
Evidence Sentence: MEK-1 and ERK-1/2 mRNA expression in each treatment group showed a downward trend, and the ERK-1/2 mRNA levels were significantly lower in the mild moxibustion group.
Evidence Sentence: It indicates that Ras, Raf-1, MEK-1, and ERK-1/2 are involved in the process of intestinal fibrosis in CD rats.
Evidence Sentence: Moxibustion can downregulate the abnormal expression of colonic Ras, Raf-1, MEK-1, and ERK-1/2 protein and mRNA levels in CD intestinal fibrosis in rats.
Evidence Sentence: The ERK-1/2 Expression in the Colonic Mucosa of Rat
Evidence Sentence: As shown in Figures 12 and 13, the phosphorylation of ERK-1/2 significantly increased in the MC group compared with the NC group (P < 0.01) and that the levels of phosphorylated ERK-1/2 proteins were significantly reduced after the MM and HSM treatments (P < 0.01).
Evidence Sentence: The results in Figure 14 show that the ERK-1/2 mRNA expression in the MC group was significantly higher than that in the NC group (P < 0.01) and that ERK-1/2 mRNA expression was significantly lower in the MM group after acupuncture treatment compared with the MC group (P < 0.05).