Evidence Details for Mbp
PMID Title Journal Year Abstract
22984478 Proteomic response to acupuncture treatment in spontaneously hypertensive rats. PLoS One. 2012;7(9):e44216. doi: 10.1371/journal.pone.0044216. Epub 2012 Sep 12. 2012 Previous animal and clinical studies have shown that acupuncture is an effective alternative treatment in the management of hypertension, but the mechanism is unclear. This study investigated the proteomic response in the nervous system to treatment at the Taichong (LR3) acupoint in spontaneously hypertensive rats (SHRs). Unanesthetized rats were subject to 5-min daily acupuncture treatment for 7 days. Blood pressure was monitored over 7 days. After euthanasia on the 7(th) day, rat medullas were dissected, homogenized, and subject to 2D gel electrophoresis and MALDI-TOF analysis. The results indicate that blood pressure stabilized after the 5th day of acupuncture, and compared with non-acupoint treatment, Taichong-acupunctured rat's systolic pressure was reduced significantly (P<0.01), though not enough to bring blood pressure down to normal levels. The different treatment groups also showed differential protein expression: the 2D images revealed 571 +/- 15 proteins in normal SD rats' medulla, 576 +/- 31 proteins in SHR's medulla, 597 +/- 44 proteins in medulla of SHR after acupuncturing Taichong, and 616 +/- 18 proteins in medulla of SHR after acupuncturing non-acupoint. In the medulla of Taichong group, compared with non-acupoint group, seven proteins were down-regulated: heat shock protein-90, synapsin-1, pyruvate kinase isozyme, NAD-dependent deacetylase sirtuin-2, protein kinase C inhibitor protein 1, ubiquitin hydrolase isozyme L1, and myelin basic protein. Six proteins were up-regulated: glutamate dehydrogenase 1, aldehyde dehydrogenase 2, glutathione S-transferase M5, Rho GDP dissociation inhibitor 1, DJ-1 protein and superoxide dismutase. The altered expression of several proteins by acupuncture has been confirmed by ELISA, Western blot and qRT-PCR assays. The results indicate an increase in antioxidant enzymes in the medulla of the SHRs subject to acupuncture, which may provide partial explanation for the antihypertensive effect of acupuncture. Further studies are warranted to investigate the role of oxidative stress modulation by acupuncture in the treatment of hypertension."

Evidence Sentence: In the medulla of the Taichong group, compared with model group, six proteins were significantly down-regulated: synapsin-1, myelin basic protein (MBP), pyruvate kinase isozyme, protein kinase C inhibitor protein 1 (KCIP-1), electron transfer flavinprotein subunit-alpha (alpha-ETF), and NAD-dependent deacetylase sirtuin-2 (SIRT2).
Evidence Sentence: In the medulla of Taichong group, compared with non-acupoint group, seven proteins were down-regulated: HSP90, synapsin-1, pyruvate kinase isozyme, SIRT2, KCIP-1, UCHL1, and MBP.
Evidence Sentence: Seven proteins were up-regulated: HSP90, synapsin-1, pyruvate kinase isozymes, SIRT2, alpha-EFT, KCIP-1, and MBP (Table 4).
Evidence Sentence: In the medulla of Taichong group, compared with normal group, two proteins were down-regulated: UCHL1 and MBP.
Evidence Sentence: In the medulla of non-acupoint group, compared with the normal group, four proteins were down -regulated: GLUD1, ALDH2, GSTM5 and SOD; six proteins were up-regulated: HSP90, synapsin-1, SIRT2, KCIP-1, DJ-1 and MBP (Table 4).
Evidence Sentence: To further verify the reliability of the proteomic analysis, the expression of synapsin I and MBP at protein and mRNA levels were analyzed by Western blot and qRT-PCR, respectively.
Evidence Sentence: MBP was also significantly increased in 9-week-old SHRs compared to age-matched normotensive SD rats, and remained unchanged after acupuncture treatment at non-acupoint but was down-regulated after acupuncture treatment at Taichong point.
Evidence Sentence: The mRNA of MBP was down-regulated in SHRs compared to age-matched normotensive SD rats, and remained unchanged after acupuncture treatment at non-acupoint but were up-regulated after acupuncture treatment at Taichong point.
Evidence Sentence: Although the results of MBP by Western blot assay were consistent with those by 2-D gel analysis, but were opposite to those from qRT-PCR.
Evidence Sentence: In the medulla of Taichong group, compared with non-acupoint group, seven proteins were down-regulated: heat shock protein-90, synapsin-1, pyruvate kinase isozyme, NAD-dependent deacetylase sirtuin-2, protein kinase C inhibitor protein 1, ubiquitin hydrolase isozyme L1, and myelin basic protein.