Evidence Details for Mstn
PMID Title Journal Year Abstract
27942989 Efficacy of femtosecond lasers for application of acupuncture therapy. Lasers Med Sci. 2017 Dec;32(9):2167-2171. doi: 10.1007/s10103-016-2124-3. Epub 2016 Dec 10. 2017 Dec Acupuncture treatment utilizes the stimulation of metal acupuncture needles that are manually inserted into a living body. In the last decades, laser light has been used as an alternative to needles to stimulate acupuncture points. We previously reported suppression of myostatin (Mstn) gene expression in skeletal muscle by means of femtosecond laser (FL) irradiation, after electroacupuncture, in which acupuncture needles are stimulated with a low-frequency microcurrent. The purpose of the study here was to investigate the efficacy of FL irradiation in mouse skeletal muscle with regard to protein synthesis. After irradiation of the hindlimbs, we first analyzed Mstn gene expression and Mstn protein level in the skeletal muscle. We then evaluated phosphorylation of the mammalian target of rapamycin (mTOR) and its downstream target 70-kDa ribosomal protein S6 kinase (p70S6K). The results showed that FL irradiation significantly reduced the amount of Mstn protein and enhanced the phosphorylation of p70S6K in of the mTOR/S6K signaling pathway. We suggest that FL irradiation activated the protein synthetic pathway in the skeletal muscle. In conclusion, we determined that FL irradiation can serve as an alternative for acupuncture needles and has the potential of being a new non-invasive acupuncture treatment of skeletal muscle."

Evidence Sentence: We previously reported suppression of myostatin (Mstn) gene expression in skeletal muscle by means of femtosecond laser (FL) irradiation, after electroacupuncture, in which acupuncture needles are stimulated with a low-frequency microcurrent.
Evidence Sentence: After irradiation of the hindlimbs, we first analyzed Mstn gene expression and Mstn protein level in the skeletal muscle.
Evidence Sentence: The results showed that FL irradiation significantly reduced the amount of Mstn protein and enhanced the phosphorylation of p70S6K in of the mTOR/S6K signaling pathway.
Evidence Sentence: FL irradiation of the skeletal muscle suppressed Mstn gene expression (Fig.
Evidence Sentence: Because suppression of gene expression is not correlated with a reduction in the amount of protein, we studied the amount of Mstn protein by using Western blotting.
Evidence Sentence: 2b shows, the FL group had a significantly decreased Mstn protein level compared with the controls.
Evidence Sentence: To examine the influence of the reduced Mstn protein on the mTOR/S6K pathway, we investigated the activation of mTOR and p70S6K in the skeletal muscle after FL irradiation.
Evidence Sentence: We previously reported suppression of myostatin (Mstn) gene expression in skeletal muscle by means of femtosecond laser (FL) irradiation, after electroacupuncture, in which acupuncture needles are stimulated with a low-frequency microcurrent.