Evidence Details for Mtor
PMID Title Journal Year Abstract
26991319 Anti-Inflammatory Effects of Acupuncture Stimulation via the Vagus Nerve. PLoS One. 2016 Mar 18;11(3):e0151882. doi: 10.1371/journal.pone.0151882. eCollection 2016. 2016 Although acupuncture therapy is widely used in traditional Asian medicine for the treatment of diverse internal organ disorders, its underlying biological mechanisms are largely unknown. Here, we investigated the functional involvement of acupuncture stimulation (AS) in the regulation of inflammatory responses. TNF-alpha production in mouse serum, which was induced by lipopolysaccharide (LPS) administration, was decreased by manual acupuncture (MAC) at the zusanli acupoint (stomach36, ST36). In the spleen, TNF-alpha mRNA and protein levels were also downregulated by MAC and were recovered by using a splenic neurectomy and a vagotomy. c-Fos, which was induced in the nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) by LPS and electroacupuncture (EAC), was further increased by focal administration of the AMPA receptor blocker CNQX and the purinergic receptor antagonist PPADS. TNF-alpha levels in the spleen were decreased by CNQX and PPADS treatments, implying the involvement of inhibitory neuronal activity in the DVC. In unanesthetized animals, both MAC and EAC generated c-Fos induction in the DVC neurons. However, MAC, but not EAC, was effective in decreasing splenic TNF-alpha production. These results suggest that the therapeutic effects of acupuncture may be mediated through vagal modulation of inflammatory responses in internal organs."

Evidence Sentence: We found the protein clearance arose from the activation of the autophagy-lysosome pathway (ALP) in a mammalian target of rapamycin (mTOR)-independent approach.
Evidence Sentence: Acupuncture promotes mTOR-independent autophagic clearance of aggregation-prone proteins in mouse brain
Evidence Sentence: Whereas acupuncture and rapamycin, a chemical mTOR inhibitor, show comparable alpha-syn clearance and therapeutic effects in the PD mouse model, the latter adopts a distinctly different, mTOR-dependent, autophagy induction process.
Evidence Sentence: Since LC3II and LAMP1 are both downstream ALP proteins, we further examined the acupuncture effects on the expression of upstream ALP proteins of p-mTOR, p-P70S6K and ULK1 (Fig.
Evidence Sentence: However, these upstream proteins were minimally affected by acupuncture, suggesting that its regulating effects on ALP are independent of mTOR.
Evidence Sentence: Nevertheless, rapamycin could significantly modulate the expression of upstream ALP proteins including p-mTOR, p-P70S6K and ULK1 (Fig.