| Evidence Sentence: |
Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin+, BrdU+ and nestin/BrdU+) were found in MCAO rats which had not been observed in normal group and sham-operated group. |
| Evidence Sentence: |
BrdU co-labeled with nestin (a special marker for neural stem cells in central nervous system (CNS)) were employed for labeling neural stem cells. |
| Evidence Sentence: |
Furthermore, RT-PCR was used to quantify the mRNA expression of nestin in cortex, hippocampus, and striatum, respectively. |
| Evidence Sentence: |
By immunofluorescence double-staining, a large number of BrdU+, nestin+, and BrdU/nestin+ cells in MCAO groups were observed as shown in Figure 5, whereas no immune-positive cells were found in normal and sham-operated group. |
| Evidence Sentence: |
However, no distinct difference was found in nestin+ and BrdU/nestin+ between no-acupuncture group and model group (shown in Figures 5(c) and 5(d)). |
| Evidence Sentence: |
Consistent with the results of immunofluorescence double-staining, model group shown a high expression of nestin mRNA compared to normal and sham-operated group especially in striatum followed by hippocampus and cortex suggesting there was different expression tendency in three cerebral regions after ischemia. |
| Evidence Sentence: |
However, nestin expression of no-acupuncture group increased in cortex and hippocampus but decreased in striatum (shown in Figure 6). |
| Evidence Sentence: |
From nestin expression of cortex, hippocampus, and striatum, we found that different cerebral regions shown different self-repair capacity. |
| Evidence Sentence: |
And the self-repair capacity could be enhanced by acupuncture stimulation; as demonstrated in Figure 6, nestin mRNA was significantly increased in cortex while slightly increased in hippocampus and decreased in striatum compared to model group and no-acupuncture group. |
