| Evidence Sentence: |
Electro-acupuncture decreases 5-HT, CGRP and increases NPY in the brain-gut axis in two rat models of Diarrhea-predominant irritable bowel syndrome(D-IBS) |
| Evidence Sentence: |
To examine whether electro-acupuncture (EA) could decrease 5-hydroxytryptamine (5-HT) and calcitonin gene-related peptide (CGRP), and increase neuro-peptide Y (NPY) in the brain-gut axis (BGA) in D-IBS using rat models. |
| Evidence Sentence: |
The content of 5-HT, CGRP and NPY in the distal colon, spinal cord, hypothalamus was examined at the end of the treatment. |
| Evidence Sentence: |
The two rat models exhibited similar characteristics, e.g., increased number of fecal pellets expelled in 1 h, decreased sacchar-intake, decreased CRD, elevated 5-HT, CGRP content and decreased NPY in the distal colon, spinal cord, hypothalamus (P < 0.05 vs. |
| Evidence Sentence: |
In comparison to 5-HT, CGRP and NPY (particularly in the spinal cord) had closer relationship with the D-IBS symptoms induced by either stress factors or Senna decotion. |
| Evidence Sentence: |
The CAS and ISC rats had higher 5-HT, CGRP content and lower NPY content in the colon, the spinal cord, the hypothalamus (P < 0.05 vs. |
| Evidence Sentence: |
NPY content was increased by EA (P < 0.05, vs. |
| Evidence Sentence: |
EA treatment reduced 5-HT, CGRP content and increased NPY content in both CAS and ISC rats (Fig. |
| Evidence Sentence: |
NPY content of colon was proportional to CGRP content of colon and spinal cord (R = 0.652, r2 = 0.425, R2 = 0.397), and could be described using the following equation: Y = 1240.389 + 45.471X1-37.872X2, in which the NPY content of colon and X1 and X2 are CGRP content of colon and spinal cord respectively. |
| Evidence Sentence: |
T value for regression between the CGRP content of hypothalamus and the NPY content of colon was -0.872 (P = 0.387). |
| Evidence Sentence: |
With component matrix = +-0.5 as weight, CGRP of spinal cord, CRD measurement, fecal pellets, sacchar-intake amount, CGRP of hypothalamus, CGRP of distal colon, 5-HT of spinal cord, NPY of hypothalamus were significant in the f1 factors. |
| Evidence Sentence: |
F3 was important in the sacchar-intake, NPY content of colon and hypothalamus, CGRP and 5-HT content of spinal cord. |