Evidence Details for Oprd1
PMID Title Journal Year Abstract
19756387 delta-Opioid receptors protect from anoxic disruption of Na+ homeostasis via Na+ channel regulation. Cell Mol Life Sci. 2009 Nov;66(21):3505-16. doi: 10.1007/s00018-009-0136-x. 2009 Nov Hypoxic/ischemic disruption of ionic homeostasis is a critical trigger of neuronal injury/death in the brain. There is, however, no promising strategy against such pathophysiologic change to protect the brain from hypoxic/ischemic injury. Here, we present a novel finding that activation of delta-opioid receptors (DOR) reduced anoxic Na+ influx in the mouse cortex, which was completely blocked by DOR antagonism with naltrindole. Furthermore, we co-expressed DOR and Na+ channels in Xenopus oocytes and showed that DOR expression and activation indeed play an inhibitory role in Na+ channel regulation by decreasing the amplitude of sodium currents and increasing activation threshold of Na+ channels. Our results suggest that DOR protects from anoxic disruption of Na+ homeostasis via Na+ channel regulation. These data may potentially have significant impacts on understanding the intrinsic mechanism of neuronal responses to stress and provide clues for better solutions of hypoxic/ischemic encephalopathy, and for the exploration of acupuncture mechanism since acupuncture activates opioid system."

Evidence Sentence: delta-opioid receptors protect from anoxic disruption of Na+ and K+ homeostasis via Na+ channel regulation
Evidence Sentence: Furthermore, we show for the first time with direct evidence that DOR expression/activation indeed plays an inhibitory role in Na+ channel regulation by decreasing the amplitude of sodium currents and increasing activation threshold of Na+ channels.
Evidence Sentence: Therefore, we applied TTX, a potent and specific voltage-gated Na+ channel blocker, to the cortical slices and tested its effect on DOR protection from anoxic K+ leakage.
Evidence Sentence: DOR expression and activation down-regulated Na+ channel function
Evidence Sentence: To determine the role of DOR in Na+ channel regulation, we co-expressed Na1.2 channels and DOR in the oocytes and then recorded sodium currents.
Evidence Sentence: 3.5 DOR activation reduced Na+ channel currents in a dose-response manner