Evidence Details for Oprl1
PMID Title Journal Year Abstract
23573158 Warming Moxibustion Relieves Chronic Visceral Hyperalgesia in Rats: Relations to Spinal Dynorphin and Orphanin-FQ System. Evid Based Complement Alternat Med. 2013;2013:920675. doi: 10.1155/2013/920675. Epub 2013 Mar 16. 2013 As a twin therapy of acupuncture in traditional Chinese medicine, moxibustion has shown its effects in relieving abdominal pain in irritable bowel syndrome (IBS) patients and IBS rat models, but its mechanisms are largely unknown. In this paper, we determined the role of spinal dynorphin and orphanin-FQ system in analgesic effect of warming moxibustion (WM) on chronic visceral hyperalgesia (CVH) in IBS-like rat model. Here, we show that (1) repeated WM at bilateral ST25 and ST37 acupoints markedly attenuated the abdominal withdrawal reflex scores in CVH rats; (2) intrathecal administration of kappa receptor antagonist prior to WM significantly attenuated the WM analgesia and dynorphinA (1-17) enhanced the WM analgesia. WM significantly reinforced the upregulation of spinal dynorphin mRNA/protein and kappa receptor mRNA levels in CVH rats; (3) intrathecal administration of orphanin-FQ receptor antagonist prior to WM significantly attenuated the WM analgesia and orphanin-FQ enhanced the WM analgesia. WM reinforced the upregulation of spinal orphanin-FQ mRNA/protein and orphanin-FQ receptor mRNA levels in CVH rats. These results suggest that moxibustion may relieve CVH at least in part by activating spinal dynorphin and orphanin-FQ system."

Evidence Sentence: Experiment 3: Moxibustion Exert Analgesic Effect by Activating Spinal OFQ-OFQ Receptor System
Evidence Sentence: As shown in Figure 6(a), at different levels of CRD stimuli (2.66, 5.32, 7.98, and 10.64 kPa), the AWR scores in model group were higher than that in OFQ group (P < 0.01, P < 0.05).
Evidence Sentence: The AWR scores were higher in OFQ+Nphe1 group than that in OFQ group (P < 0.01, P < 0.05).
Evidence Sentence: OFQ alleviated the CRD-induced CVH by binding to the OFQ receptor.
Evidence Sentence: As shown in Figure 6(b), at different levels of CRD stimuli, an obvious increase of AWR scores was shown in Nphe1+WM group compared to WM group (P < 0.01, P < 0.05), and the AWR scores in OFQ+WM group was lower than that in WM group (P < 0.05).
Evidence Sentence: OFQ could enhance it.
Evidence Sentence: The expression of OFQ and of its receptor in L6-S1 spinal dorsal horn was also tested by ISH, immunohistochemistry and ELISA.
Evidence Sentence: As shown in Figure 7, ISH examination revealed that the expression of OFQ mRNA in model group was higher than that in normal group (P < 0.01), and WM treatment further produced a marked elevation of OFQ mRNA (P < 0.01).
Evidence Sentence: As shown in Figure 8, the level of OFQ protein was higher in model group than that in normal group (P < 0.05), and significantly increased after WM treatment (P < 0.05).
Evidence Sentence: ISH showed that the expression of spinal OFQ receptor mRNA increased during CVH and was further remarkably enhanced by WM treatment (P < 0.01) (Figure 9).
Evidence Sentence: These data indicated that WM treatment induced the expression of OFQ and enhanced the transcription of OFQ receptor mRNA in CVH rats.
Evidence Sentence: Experiment 3: Moxibustion Exert Analgesic Effect by Activating Spinal OFQ-OFQ Receptor System
Evidence Sentence: OFQ alleviated the CRD-induced CVH by binding to the OFQ receptor.
Evidence Sentence: These data indicated that WM treatment induced the expression of OFQ and enhanced the transcription of OFQ receptor mRNA in CVH rats.
Evidence Sentence: Warming Moxibustion Relieves Chronic Visceral Hyperalgesia in Rats: Relations to Spinal Dynorphin and Orphanin-FQ System
Evidence Sentence: In this paper, we determined the role of spinal dynorphin and orphanin-FQ system in analgesic effect of warming moxibustion (WM) on chronic visceral hyperalgesia (CVH) in IBS-like rat model.
Evidence Sentence: WM significantly reinforced the upregulation of spinal dynorphin mRNA/protein and kappa receptor mRNA levels in CVH rats; (3) intrathecal administration of orphanin-FQ receptor antagonist prior to WM significantly attenuated the WM analgesia and orphanin-FQ enhanced the WM analgesia.
Evidence Sentence: These results suggest that moxibustion may relieve CVH at least in part by activating spinal dynorphin and orphanin-FQ system.
Evidence Sentence: WM reinforced the upregulation of spinal orphanin-FQ mRNA/protein and orphanin-FQ receptor mRNA levels in CVH rats.
Evidence Sentence: WM reinforced the upregulation of spinal orphanin-FQ mRNA/protein and orphanin-FQ receptor mRNA levels in CVH rats.
Evidence Sentence: WM significantly reinforced the upregulation of spinal dynorphin mRNA/protein and kappa receptor mRNA levels in CVH rats; (3) intrathecal administration of orphanin-FQ receptor antagonist prior to WM significantly attenuated the WM analgesia and orphanin-FQ enhanced the WM analgesia.