Evidence Details for Ppara
PMID Title Journal Year Abstract
28806763 Genome-wide regulation of electro-acupuncture on the neural Stat5-loss-induced obese mice. PLoS One. 2017 Aug 14;12(8):e0181948. doi: 10.1371/journal.pone.0181948. eCollection 2017. 2017 Acupuncture is reported to be effective in treating obesity related illnesses, but its mechanism is still unclear. To investigate this mechanism we applied electro-acupuncture (EA) in a mouse model of obesity and used RNA-seq to identify molecular consequences. Deletion of the transcription factor STAT5 from neurons (Stat5NKO) led to obesity. Acupuncture, in turn, reduced body weight and the ratio of epididymal white adipose tissue (Epi-WAT) to body weight, and it also decreased plasma concentrations of glucose, triglyceride, and cholesterol. In addition, EA increased cold endurance of Stat5NKO obese mice. EA reversed altered gene expressions in the hypothalamus and Epi-WAT, especially in the hypothalamus in Stat5NKO obese mice. This study provides, for the first time, insight into genomic networks of obesity and their modulation by electro-acupuncture, which in turn reveals potential mechanisms that explain acupuncture-induced weight-loss."

Evidence Sentence: KEGG pathway analysis showed that EA down-regulated 370 genes were enriched in 16 pathways, including substance metabolism-especially lipid metabolism-such as PPAR signaling, adipocytokine signaling, fatty acid metabolism, insulin signaling, and glycerophopholipid metabolism.
Evidence Sentence: Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed that these up-regulated Differentially Expressed Genes (DEGs) belonged to extracellular matrix (ECM)-receptor interaction, complement and coagulation cascades, PPAR (Peroxisome Proliferator Activated Receptor) signaling pathway, adipocytokine signaling pathway, insulin signaling pathway, and several substance metabolism, which are closely related to lipids metabolism (Fig 2B).
Evidence Sentence: KEGG pathway analysis suggested that these up-regulated genes were enriched in obesity-related pathways, including glycolysis, TCA (Tricarboxylic acid) cycle, fructose and mannose metabolism, PPAR signaling pathway, steroid biosynthesis, and fatty acid biosynthesis (Fig 2E).
Evidence Sentence: leukocyte transendothelial migration), PPAR signaling pathway, O-glycan biosynthesis, and drug metabolism (Fig 2F).
Evidence Sentence: KEGG analysis showed that they mainly participated in glycerophospholiplid metabolism, glycerolipid metabolism, PPAR signaling pathway, and ether lipid metabolism, which contributed to lipid metabolism (Fig 3D).
Evidence Sentence: Compared with the up-regulated gene pathways in the Stat5NKO group, except for the valine, leucine, and isoleucine degradation pathways, EA treatment modified 15 out of 16 pathways, and PPAR signaling pathway were listed as the most enriched pathway in the Stat5NKO+EA group (Fig 4C).
Evidence Sentence: Examining the PPAR signaling pathway revealed that most of the involved DEGs were directly reversed by EA (Fig 4D).
Evidence Sentence: The up-regulated 361 genes were enriched in 8 KEGG pathways, and 4 of these pathways (leukocyte transendothelial migration, tight junction, PPAR signaling pathway, and CAMs) were seen in the Stat5NKO induced down-regulated pathways (Fig 4F).