Evidence Details for Pura
| PMID | Title | Journal | Year | Abstract |
|---|---|---|---|---|
| 23970931 | Proteomic analysis of the effect of acupuncture on the suppression of kainic Acid-induced neuronal destruction in mouse hippocampus. | Evid Based Complement Alternat Med. 2013;2013:436315. doi: 10.1155/2013/436315. Epub 2013 Jul 23. | 2013 | Kainic acid (KA) is a neurotoxin that induces epileptic seizures and excitotoxicity in the hippocampus. Acupuncture is frequently used as an alternative therapy for epilepsy, and it has been known to protect hippocampal neurons against KA toxicity. Using proteomic analysis, we investigated protein expression changes in the hippocampus following acupuncture stimulation at HT8. Eight-week-old male C57BL/6 mice (20-25 g) received acupuncture treatment at HT8 acupoint bilaterally once a day for 3 days and were then administered KA (30 mg/kg) intraperitoneally. Twenty-four hours after KA injection, neuronal survival and astrocyte activation in the hippocampus were measured, and protein expression in the hippocampus was identified by 2-dimensional electrophoresis. Acupuncture stimulation at HT8 suppressed KA-induced neuronal death and astrocyte activation in the hippocampus. We identified the changes in the expression of 11 proteins by KA or acupuncture stimulation at HT8 and found that acupuncture stimulation at HT8 normalized the expression of dihydropyrimidinase-related protein 2 and upregulated the expression of transcriptional activator protein pur-alpha, serine/threonine-protein phosphatase 5, and T-complex protein 1 subunit alpha, which are related to the survival of neurons. These results suggest that acupuncture stimulation at HT8 changes protein expression profiles in the hippocampus in favor of neuronal survival in KA-treated mice." |
| Evidence Sentence: | After matching the replicated maps, differential changes in intensities among the mice in the saline, KA and KA + HT8 groups were limited to 11 proteins: valosin containing protein (VCP), ubiquitin-like modifier-activating enzyme 1 isoform 1 (ULMAE-1), ATP synthase subunit d (ATPS), heat shock 70 kDa protein 4L (HSP70), heat shock protein 4 like, isoform CRA_c (HSP4L), dihydropyrimidinase-related protein 2 (CRMP-2), transcriptional activator protein pur-alpha (PURA), pyruvate dehydrogenase protein X component (PDX), serine/threonine-protein phosphatase 5 (PP5), T-complex protein 1 subunit alpha (TCP-1alpha), and uncharacterized protein LOC433182 (LOC433182). |
| Evidence Sentence: | The levels of PURA, PDX, PP5, TCP-1alpha, and LOC433182 were unchanged by KA injection, but acupuncture stimulation at HT8 significantly increased them (Table 1, Figures 3(c) and 3(d)). |
| Evidence Sentence: | To verify the reliability of the proteomics analysis, PURA and PP5 were selected as representative proteins and subjected to western blotting. |
| Evidence Sentence: | We identified the changes in the expression of 11 proteins by KA or acupuncture stimulation at HT8 and found that acupuncture stimulation at HT8 normalized the expression of dihydropyrimidinase-related protein 2 and upregulated the expression of transcriptional activator protein pur-alpha, serine/threonine-protein phosphatase 5, and T-complex protein 1 subunit alpha, which are related to the survival of neurons. |