| Evidence Sentence: |
Large inward currents could be recorded after the injection of Nav1.2 cRNA (Figure 3). |
| Evidence Sentence: |
We measured the sodium currents in the oocytes with various amounts of injected Nav1.2 cRNA after 40 h of incubation. |
| Evidence Sentence: |
Only one out of three oocytes showed inward currents with the peak value being less than 500 nA when injected with 1 ng of Nav1.2 cRNA (Figure 4B) (n=3). |
| Evidence Sentence: |
At 5 ng of Nav1.2 cRNA, the sodium currents increased greatly with the average value of the peak currents being -1765.2+-290.5 nA (n=6) (Figure 4C). |
| Evidence Sentence: |
In the oocytes with injection of 10 ng Nav1.2 cRNA, the inward currents increased by several folds with the peak values being >6000 nA (-6604.8+-416.7 nA) (n=6) (Figure 4D). |
| Evidence Sentence: |
These results, as summarized in Figure 4E and 4F, suggest that sodium current amplitude was proportionally related to the amount of the Nav1.2 cRNA injected into the oocytes. |
| Evidence Sentence: |
For example, in the oocytes with 5 ng of Nav1.2 cRNA, the current amplitude could double or more if the culture time was extended from 40 to 72 hours or longer (Figures 5-6). |
| Evidence Sentence: |
On the other hand, UFP-512 (5 microM) had no significant effect on sodium currents in the oocytes expressing Nav1.2 alone (5 ng Nav1.2 cRNA) (p>0.05, n=10, data not shown). |
| Evidence Sentence: |
In general, the peak current was around 0 mV of the potential in the oocytes with 5 ng of Nav1.2 cRNA alone (Figure 3). |
