Evidence Details for Slc6a13
PMID Title Journal Year Abstract
25983633 Neuroprotective changes in degeneration-related gene expression in the substantia nigra following acupuncture in an MPTP mouse model of Parkinsonism: Microarray analysis. Genet Mol Biol. 2015 Mar;38(1):115-27. doi: 10.1590/S1415-475738120140137. Epub 2014 Mar 17. 2015 Mar Parkinson's disease (PD) is a neurodegenerative disorder characterized by the death of dopamine-generating cells in the substantia nigra (SN). Acupuncture stimulation results in an enhanced survival of dopaminergic neurons in the SN in Parkinsonism animal models. The present study investigated changes in gene expression profiles measured using whole transcript array in the SN region related to the inhibitory effects of acupuncture in a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Parkinsonism model. In this model, acupuncture stimulation at GB34 and LR3 attenuated the decrease in tyrosine hydroxylase in the SN region; stimulation at non-acupoints did not suppress this decrease. Gene array analysis revealed that 22 (10 annotated genes: Cdh1, Itih2, Mpzl2, Rdh9, Serping1, Slc6a13, Slc6a20a, Slc6a4, Tph2, and Ucma) probes that were up-regulated in MPTP animals relative to controls were exclusively down-regulated by acupuncture stimulation. In addition, 17 (two annotated genes: 4921530L21Rik and Gm13931) probes that were down-regulated in MPTP animals compared to controls were exclusively up-regulated by acupuncture stimulation. These findings indicate that the 39 probes (12 annotated genes) affected by MPTP and acupuncture may be responsible for the inhibitory effects of acupuncture on degeneration-related gene expression in the SN following damage induced by MPTP intoxication."

Evidence Sentence: Cdh1 signaling is an important component of the regulation of GABAergic synapses in brain neurons; indeed, elevated GABA levels have been detected in PD.
Evidence Sentence: Solute carrier family 6 (neurotransmitter transporter, GABA), member 13 (Slc6a13) is a sodium- and chloride-dependent member of solute carrier family 6 (SLC6) that mediates the rapid removal of GABA and maintains low extracellular levels of this neurotransmitter.
Evidence Sentence: Evidence suggests that alteration of GABA transporters is linked to PD.
Evidence Sentence: Accordingly, the overstimulation of GABAA receptors can potentiate neuronal cell damage; GABA transport blockers are neuroprotective in these situations , suggesting that overexpression of the GABA transporter can damage neurons.
Evidence Sentence: Gene array analysis revealed that 22 (10 annotated genes: Cdh1, Itih2, Mpzl2, Rdh9, Serping1, Slc6a13, Slc6a20a, Slc6a4, Tph2, and Ucma) probes that were up-regulated in MPTP animals relative to controls were exclusively down-regulated by acupuncture stimulation.
Evidence Sentence: Solute carrier family 6 (neurotransmitter transporter, GABA), member 13 (Slc6a13) is a sodium- and chloride-dependent member of solute carrier family 6 (SLC6) that mediates the rapid removal of GABA and maintains low extracellular levels of this neurotransmitter.
Evidence Sentence: In the current study, Slc6a13 was up-regulated in the MPTP group compared to the control group; therefore this may be the underlying cause of neuronal intoxication.
Evidence Sentence: However, acupuncture stimulation at GB34 and LR3 maintained Slc6a13 gene expression at levels similar to those in control animals, suggesting that acupuncture has a neuroprotective effect.
Evidence Sentence: Slc6a13 was involved in GABAergic synapse, albeit not significantly so.
Evidence Sentence: These findings demonstrate that both MPTP and acupuncture at acupoints influence Cdh1, Tph2, Slc6a4, Serping1, Slc6a13, and Gm13931 expression in the indicated pathways in the SN region.