Evidence Details for Th
PMID Title Journal Year Abstract
31871478 Effects of Combined Treatment with Acupuncture and Chunggan Formula in a Mouse Model of Parkinson's Disease. Evid Based Complement Alternat Med. 2019 Nov 21;2019:3612587. doi: 10.1155/2019/3612587. eCollection 2019. 2019 Parkinson's disease is the second most common neurodegenerative disease. Patients with Parkinson's disease can be treated with a combination of acupuncture and herbal medicine, but studies on the synergistic effects of the combined treatment have not yet been conducted. Thus, we subjected an MPTP-induced Parkinson's disease mouse model to the combined treatment. We used acupoint GB34 for acupuncture and modified Chunggantang (KD5040) as the herbal medicine, as they have been reported to be effective in Parkinson's disease. We investigated the suboptimal dose of KD5040 and then used this dose in the combined treatment. The results showed that the combined treatment had a synergistic effect on improvements in abnormal motor function and neurodegeneration compared with the use of acupuncture or herbal medicine alone. The combined treatment also had a neuroprotective effect via the PI3K/AKT and MAPK/ERK signaling pathways. These findings suggest that the combined treatment with acupuncture and KD5040 can help improve the symptoms of Parkinson's disease."

Evidence Sentence: Statistical analysis of the TH expression on the western blot revealed that there was a significant difference in the striatum (F3, 12 = 315.0, p < 0.0001) and SNpc (F3, 12 = 5541, p < 0.0001) among whole groups.
Evidence Sentence: The TH expression of the M + KDH group is higher than that of the control group, but there is no significant difference in both striatum and SNpc.
Evidence Sentence: The TH expression in the M group decreased significantly in both regions compared with those in the C group (p < 0.001).
Evidence Sentence: The TH expression in the M + KDL and the M + KDH groups showed a significant increase in both two regions relative to those in the M group (p < 0.001) (Figures 3(b) and 4(b)).
Evidence Sentence: To quantify TH-positive cells, we measured the OD values of TH-positive dopaminergic nerve fibers in the striatum (Figure 8) and counted the number of TH-positive dopaminergic neurons in the SNpc (Figure 9).
Evidence Sentence: One-way ANOVA of the OD value in the striatum (F4, 15 = 257.5, p < 0.0001) and the number of TH-positive dopaminergic neurons in the SNpc (F4, 15 = 73.40, p < 0.0001) showed a significant difference among the whole experimental groups.
Evidence Sentence: Statistical analysis of the TH expression on the western blot revealed a significant difference among the whole groups in the striatum (F4, 15 = 230.0, p < 0.0001) and SNpc (F4, 15 = 3494, p < 0.0001).
Evidence Sentence: The loss of TH protein induced by MPTP in the M group decreased largely compared with the C group in both striatum and SNpc (p < 0.001).
Evidence Sentence: The loss of TH protein was inhibited by both single and combined treatments, and that of the M + Acu + KDL group was inhibited largely compared with other groups (p < 0.001) (Figures 8(b) and 9(b)).