Evidence Details for Trpv1
PMID Title Journal Year Abstract
23097675 Acupuncture Alleviates Colorectal Hypersensitivity and Correlates with the Regulatory Mechanism of TrpV1 and p-ERK. Evid Based Complement Alternat Med. 2012;2012:483123. doi: 10.1155/2012/483123. Epub 2012 Oct 11. 2012 Here we used a mouse model of zymosan-induced colorectal hypersensitivity, a similar model of IBS in our previous work, to evaluate the effectiveness of the different number of times of acupuncture and elucidate its potential mechanism of EA treatment. Colorectal distension (CRD) tests show that intracolonic zymosan injection does, while saline injection does not, induce a typical colorectal hypersensitivity. EA treatment at classical acupoints Zusanli (ST36) and Shangjuxu (ST37) in both hind limbs for 15 min slightly attenuated and significantly blunted the hypersensitive responses after first and fifth acupunctures, respectively, to colorectal distention in zymosan treatment mice, but not in saline treatment mice. Western blot results indicated that ion channel and TrpV1 expression in colorectum as well as ERK1/2 MAPK pathway activation in peripheral and central nerve system might be involved in this process. Hence, we conclude that EA is a potential therapeutic tool in the treatment and alleviation of chronic abdominal pain, and the effectiveness of acupuncture analgesia is accumulative with increased number of times of acupuncture when compared to that of a single time of acupuncture."

Evidence Sentence: Acupuncture Alleviates Colorectal Hypersensitivity and Correlates with the Regulatory Mechanism of TrpV1 and p-ERK
Evidence Sentence: Western blot results indicated that ion channel and TrpV1 expression in colorectum as well as ERK1/2 MAPK pathway activation in peripheral and central nerve system might be involved in this process.
Evidence Sentence: Western blot analysis showed that there was a detectable signal of transient receptor potential vanilloid 1(TrpV1) in the colorectum (Figure 5).
Evidence Sentence: TrpV1 expression was only slightly increased in response to acupuncture in group S + A, while it was dramatically induced by zymosan injection.
Evidence Sentence: EA obviously compromises zymosan-induced upregulation of TrpV1.
Evidence Sentence: The Z + C group displayed an obviously higher expression of TrpV1 when compared with the S + C group, by 80%.
Evidence Sentence: However, the TrpV1 level in S + A group manifested 44.43% up-regulation, when compared with the S + C group.
Evidence Sentence: This in turn demonstrated that EA greatly compromised zymosan-induced up-regulation of TrpV1 by 87.58%.
Evidence Sentence: These data suggested that TrpV1 is involved in zymosan-induced visceral hypersensitivity and EA suppressed visceral hypersensitivity.
Evidence Sentence: We found that TrpV1 protein is detectable only in colorectal tissue by Western blot.
Evidence Sentence: EA treatment alone can slightly induce TrpV1 in S + A group.