Evidence Details for Trpv1
PMID Title Journal Year Abstract
34757591 Hypoglycemic Effect of Electroacupuncture at ST25 Through Neural Regulation of the Pancreatic Intrinsic Nervous System. Mol Neurobiol. 2022 Jan;59(1):703-716. doi: 10.1007/s12035-021-02609-1. Epub 2021 Nov 10. 2022 Jan Electroacupuncture (EA) is considered to have potential antidiabetic effects; however, the role of the pancreatic intrinsic nervous system (PINS) in EA-induced amelioration of type 2 diabetes (T2DM) remains unclear. Therefore, we investigated whether EA at ST25 exerts any beneficial effects on insulin resistance (IR), inflammation severity, and pancreatic beta cell function via the PINS in a rat model of a high-fat diet-streptozotocin (HFD/STZ)-induced diabetes. To this end, Sprague Dawley rats were fed with HFD to induce IR, followed by STZ (35 mg/kg, i.p.) injection to establish the T2DM model. After hyperglycemia was confirmed as fasting glucose level > 16.7 mmol/L, the rats were treated with EA (2 mA, 2/15 Hz) for the next 28 days. Model rats showed increased serum glucose, insulin, IR, and TNF-alpha levels with a concomitant decrease in beta cell function. Microscopy examination of the pancreas revealed pathological changes in islets, which reverted to near-normal levels after EA at ST25. EA improved islet cell morphology by increasing islet area and reducing vacuolation. EA at ST25 decreased transient receptor potential vanilloid 1 (TRPV1) and increased substance P (SP) and calcitonin gene-related peptide (CGRP) expression. Subsequently, insulin secretion decreased and impaired pancreatic endocrine function was restored through the TRPV1 channel (SP/CGRP)-insulin circuit. EA increased choline acetyltransferase and neuropeptide Y expression and controlled inflammation. It also enhanced the cocaine and amphetamine-regulated transcript prepropeptide expression and promoted glucagon-like peptide-1 secretion. Additionally, the electrophysiological activity of PINS during acupuncture (2.71 +/- 1.72 Hz) was significantly increased compared to the pre-acupuncture frequency (0.32 +/- 0.37 Hz, P < 0.05). Thus, our study demonstrated the beneficial effect of EA on beta cell dysfunction via the PINS in rat models of HFD-STZ-induced T2DM."

Evidence Sentence: EA at ST25 decreased transient receptor potential vanilloid 1 (TRPV1) and increased substance P (SP) and calcitonin gene-related peptide (CGRP) expression.
Evidence Sentence: Subsequently, insulin secretion decreased and impaired pancreatic endocrine function was restored through the TRPV1 channel (SP/CGRP)-insulin circuit.
Evidence Sentence: Pancreatic Endocrine Function Was Restored Through the TRPV1-(SP/CGRP)-beta Cell Circuit
Evidence Sentence: We focused on the expression of CGRP and SP since they can be regulated through transient receptor potential vanilloid 1(TRPV1).
Evidence Sentence: On the basis of the findings obtained with the observation of insulin in rat pancreas, we further evaluated the results of immunofluorescence staining and identified the expression of TRPV1 and insulin in rat pancreas to explore the neural regulation of pancreatic endocrine secretion through EA (Fig.
Evidence Sentence: In comparison with the model control group, the model group showed an increased fluorescently stained area of TRPV1 and a reduced area in the EA group.
Evidence Sentence: Quantification of TRPV1, CGRP, and SP in rat pancreas was performed by WB and shown in Fig.