| Evidence Sentence: |
WAI stimulation at PC-6 increased the number of dorsal root ganglia double-stained with TRPV1 and CGRP. |
| Evidence Sentence: |
To explore which subtypes of TRPV1-expressing DRG neurons were activated by WAI stimulation at PC-6, double-staining of TRPV1 and the neuronal markers CGRP (a peptidergic small neuronal marker), IB4 (a nonpeptidergic small neuronal marker) or NF200 (a marker for sensory myelinated fibers) was performed. |
| Evidence Sentence: |
The fluorescence intensity of TRPV1-immunoreactive neurons colocalized with CGRP was significantly higher in the WAI-stimulated group (n = 6) than in the normal group (Figures 5A,B) (t-test, F = 3.164, p < 0.001), and the number of TRPV1 neurons double-labeled with IB4 was significantly higher in the WAI group (n = 6) than in the normal group (Figures 5E,F) (n = 6, t-test, F = 1.249, p < 0.05). |
| Evidence Sentence: |
On the other hand, few neurons were double-labeled with NF200 and TRPV1 in the either the normal group or the WAI group (Figures 5C,D). |
| Evidence Sentence: |
Inhibition of the PC-6-stimulation antihypertensive effect by ablating TRPV1 with RTX |
| Evidence Sentence: |
To observe the activation of TRPV1 by WAI stimulation at 48 C, immunohistochemistry for TRPV1 in the DRGs of C6-T2 was carried out in another set of animals. |
| Evidence Sentence: |
An almost 2-fold increase in TRPV1 was found in the WAI group compared with the normal group (t-test, F = 4.806, p < 0.0001; Figures 4C,D). |
| Evidence Sentence: |
To identify whether the ablation of peripheral TRPV1 channels at PC-6 inhibited the antihypertensive effect of WAI stimulation at PC-6, RTX, known to desensitize TRPV1 receptors, was injected into PC-6 in rats with IMH. |
| Evidence Sentence: |
Immunohistochemistry for TRPV1 showed that the enhanced expression of TRPV1 induced by WAI stimulation at PC-6 was suppressed by pretreatment with RTX prior to WAI stimulation (one-way ANOVA; F=13.22, p < 0.0001; Figures 6B,C). |
| Evidence Sentence: |
To explore whether the antihypertensive effect of WAI stimulation at PC-6 is mediated through peripheral TRPV1 receptors, the TRPV1 antagonist capsazepine was injected into the PC-6 acupoint 40 min prior to the experiment. |
| Evidence Sentence: |
To further confirm whether the activation of TRPV1 receptors at PC-6 itself can produce antihypertensive effects, the TRPV1 agonist capsaicin was injected into PC-6 10 min prior to immobilization stress. |
| Evidence Sentence: |
Such effects were reproduced by capsaicin (a TRPV1 agonist) injection into PC-6 or WAI stimulation at 48 C. In contrast, PC-6 pretreatment with the TRPV1 antagonist capsazepine blocked the antihypertensive effect of WAI stimulation at PC-6. |
