Detail information
ID ENOM00030
Accession GSE58803
Status Public on Mar 21, 2016
Title Transcript Profiling of Periaqueductal Gray in Response to Electroacupuncture on Rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary
The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and low responders (NR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in spinal dorsal horn induced by 2Hz/100Hz electroacupuncture in HR and NR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in dorsal horn. Transcriptome profiling analysis found that some co-regulated genes related with electroacupuncture or 2Hz or 100Hz fruqencies. These co-regulated genes were plasticity-related by GO analysis.
Samples
GSM ID Sample info Characteristics Description
GSM1419812 2Hz_HR_1h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419813 2Hz_HR_1h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419814 2Hz_HR_1h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 1h; strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419815 2Hz_HR_1h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419816 2Hz_HR_1h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419817 2Hz_NR_1h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419818 2Hz_NR_1h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419819 2Hz_NR_1h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419820 2Hz_NR_1h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419821 100Hz_HR_1h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419822 100Hz_HR_1h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419823 100Hz_HR_1h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419824 100Hz_HR_1h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419825 100Hz_HR_1h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419826 100Hz_NR_1h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 1h; strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419827 100Hz_NR_1h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 1h; strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419828 100Hz_NR_1h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 1h; strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419829 100Hz_NR_1h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419830 100Hz_NR_1h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 1h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 1 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419831 2Hz_HR_24h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419832 2Hz_HR_24h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419833 2Hz_HR_24h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 24h; strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419834 2Hz_HR_24h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419835 2Hz_HR_24h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419836 2Hz_NR_24h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419837 2Hz_NR_24h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419838 2Hz_NR_24h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419839 2Hz_NR_24h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419840 2Hz_NR_24h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 2Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 2Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419841 100Hz_HR_24h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419842 100Hz_HR_24h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419843 100Hz_HR_24h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419844 100Hz_HR_24h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419845 100Hz_HR_24h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: high responder(HR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419846 100Hz_NR_24h_PAG1 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419847 100Hz_NR_24h_PAG2 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419848 100Hz_NR_24h_PAG3 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419849 100Hz_NR_24h_PAG4 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM1419850 100Hz_NR_24h_PAG5 rat strain: Sprague-Dawley tissue: PAG treatment: 100Hz electroacupuncture responder type: low responder(NR) time: 24h;strain: Sprague-Dawley treatment: control tissue: PAG Channel 1: Sprague-Dawley rats were received 100Hz electroacupuncture for 30 min, nociceptive testing and returned to home cages for 24 hours before sacrificed.These rats were non-sensitivity on EA analgesia. It's PAG was then collected for transcript profiling analysis. Channel 2: strain: Sprague-Dawley rats were sacrificed after stayed in their home cages without receiving electroacupuncture stimulation.the RNA of PAG were collected for transcript profilinf analysis Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
Platform GPL3498: SBC Rat cDNA Microarray ver2.0
Indicator
Bbs4, Camk2a, Gabbr1, Gabrd, Gfap, Hrh1, Kcna1, Kif5a, Oprl1, Slc6a1, Apbb1, Abca2, Aplp2, App, Htr7, Ntsr2, Ppm1f, Prkar1b, Cdk5, Grm4
Th, Mtap6, Ccr5, Cd74, Cxcl10, Dusp1
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