Detail information
ID ENOM00039
Accession GSE21733
Status Public on May 31, 2010
Title Transcript Profiling of Spinal Dorsal Horn in Response to Electroacupuncture on Rats at 24h
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary
The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and low responders (LR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in spinal dorsal horn induced by 2Hz/100Hz electroacupuncture in HR and LR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in dorsal horn after 2Hz/100Hz electoacupunture stimulation. Transcriptome profiling analysis found that different regulation of gene expression after 2Hz/100Hz electroacupuncture in HR and LR rats at 24 hr time point. Keywords: Transcriptome analysis
Samples
GSM ID Sample info Characteristics Description
GSM542196 2Hz_HR_24h_DH1 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: high responder (HR) rats tissue type: dorsal horn (DH); strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542197 2Hz_HR_24h_DH2 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: high responder (HR) rats tissue type: dorsal horn (DH); strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542198 2Hz_HR_24h_DH3 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: high responder (HR) rats tissue type: dorsal horn (DH); strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542199 2Hz_HR_24h_DH4 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: high responder (HR) rats tissue type: dorsal horn (DH); strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542200 2Hz_HR_24h_DH5 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542201 2Hz_LR_24h_DH1 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542202 2Hz_LR_24h_DH2 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542203 2Hz_LR_24h_DH3 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542204 2Hz_LR_24h_DH4 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542205 2Hz_LR_24h_DH5 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 2Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542206 100Hz_HR_24h_DH1 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542207 100Hz_HR_24h_DH2 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542208 100Hz_HR_24h_DH3 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542209 100Hz_HR_24h_DH4 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542210 100Hz_HR_24h_DH5 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: high responder (HR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542211 100Hz_LR_24h_DH1 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542212 100Hz_LR_24h_DH2 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542213 100Hz_LR_24h_DH3 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542214 100Hz_LR_24h_DH4 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM542215 100Hz_LR_24h_DH5 rat time point: 24h strain: Sprague-Dawley electroacupuncture frequency: 100Hz group: low responder (LR) rats tissue type: dorsal horn (DH);strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation tissue type: dorsal horn (DH) Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
Platform GPL3498: SBC Rat cDNA Microarray ver2.0
Indicator
Bbs4, Camk2a, Gabbr1, Gabrd, Gfap, Hrh1, Kcna1, Kif5a, Oprl1, Slc6a1, Apbb1, Abca2, Aplp2, App, Htr7, Ntsr2, Ppm1f, Prkar1b, Cdk5, Grm4
Th, Mtap6, Ccr5, Cd74, Cxcl10, Dusp1
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