Detail information
ID ENOM00040
Accession GSE57771
Status Public on Sep 02, 2014
Title Electroacupuncture Improves Trinitrobenzene Sulphonic Acid-Induced Colitis, Evaluated by Transcriptomic Study
Organism Mus musculus
Experiment type Expression profiling by array
Summary
Inflammatory bowel disease is a chronic colonic inflammation that displays symptoms like diarrhea and weight loss. Acupuncture has been widely accepted by Western countries for the treatment of pain. In this study, we analyzed the efficacy and mechanism of electroacupuncture (EA) on trinitrobenzene sulphonic acid (TNBS)-induced colitis in mice. Mice were intrarectally administered 250 mg/kg TNBS and electroacupunctured at Quze (PC3) and Neiguan (PC6) acupoints, which have been applied for gastrointestinal disorders. Gene expression profiles in colons and spleens were analyzed by microarray for the elucidation of mechanism of EA. Our data showed that EA at PC3 and PC6 improved macroscopic and microscopic features of colitis, and the improvement displayed a frequency-dependent manner. Administration of TNBS upregulated the expression of most cytokine genes in colons, while EA downregulated the expression of TNBS-induced cytokine genes. Pathway analysis showed that EA significantly affected inflammatory pathways in colons and immunity-associated pathway in spleens. Immunohistochemical staining further showed that EA decreased the expression of interleukin-1β and nuclear factor-κB. In conclusion, this is the first study reporting the global gene expression profiles of EA on TNBS-induced colitis. Our findings suggested that inflammatory and immunity pathways were involved in the anti-inflammatory mechanism of EA on colitis induced by TNBS.
Samples
GSM ID Sample info Characteristics Description
GSM1388351 Mock_Spleen_1 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388352 Mock_Spleen_2 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388353 TNBS_Spleen_1 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388354 TNBS_Spleen_2 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388355 EA_Spleen_1 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388356 EA_Spleen_2 strain: FVB tissue: Spleen Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388357 Mock_Colon_1 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388358 Mock_Colon_2 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388359 TNBS_Colon_1 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388360 TNBS_Colon_2 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388361 EA_Colon_1 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
GSM1388362 EA_Colon_2 strain: FVB tissue: Colon Total RNAs were extracted from tissues using RNeasy Mini kit (Qiagen, Valencia, CA, USA).
Platform GPL6845: Phalanx Mouse OneArray
Indicator
IL-1beta, IL-6, TNF-alpha, IL-4, NF-kappaB, IL-13, IL-18, IL-1, TGF-beta
Download Download data in GEO database