Detail information
ID ENOM00041
Accession GSE21258
Status Public on Apr 11, 2010
Title Transcript Profiling of Spinal Dorsal Horn in Response to Electroacupuncture on Rats at 1h
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary
The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and low responders (LR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in spinal dorsal horn induced by 2Hz/100Hz electroacupuncture in HR and LR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in dorsal horn. Transcriptome profiling analysis found that some co-regulated genes related with electroacupuncture or 2Hz or 100Hz freqencies. These co-regulated genes were plasticity-related by GO analysis. We also found some special regulated genes in HR vs. LR in 2Hz/100Hz electroacupuncture stimulation. These results suggested that neurotransmitter system and cytokine different between HR with LR in 2Hz electroacupuncture. But in 100Hz electroacupunture, there were many different regulated genes related with ribosome between HR with LR, which needs more studies to research the function and may play an important role in HR vs. LR by 100Hz electroacupuncture. Keywords: Transcriptome analysis
Samples
GSM ID Sample info Characteristics Description
GSM531353 2Hz_HR_1h_DH1 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531354 2Hz_HR_1h_DH2 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531355 2Hz_HR_1h_DH3 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531356 2Hz_HR_1h_DH4 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531357 2Hz_HR_1h_DH5 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531358 2Hz_LR_1h_DH1 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531359 2Hz_LR_1h_DH2 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531360 2Hz_LR_1h_DH3 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531361 2Hz_LR_1h_DH4 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 2Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531362 100Hz_HR_1h_DH1 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531363 100Hz_HR_1h_DH2 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531364 100Hz_HR_1h_DH3 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531365 100Hz_HR_1h_DH4 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531366 100Hz_HR_1h_DH5 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: high responder (HR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531367 100Hz_LR_1h_DH1 rat time point: 1h;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: low responder (LR) rats Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531368 100Hz_LR_1h_DH2 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531369 100Hz_LR_1h_DH3 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531370 100Hz_LR_1h_DH4 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
GSM531371 100Hz_LR_1h_DH5 rat time point: 1h strain: Sprague-Dawley tissue type: spinal dorsal horn frequency: 100Hz group: low responder (LR) rats;strain: Sprague-Dawley reference: rats sacrificed after stayed in their home cages without receiving electroacupuncture stimulation. Total RNA was prepared by using TRIzol reagent (Life Technologies, USA), further purified with an RNEasy column (Qiagen, Germany), and quantified by using gel electrophoresis and spectrophotometer followed by reverse transcription labeling. Each RNA sample was reverse-transcribed into cDNA primed with oligo(dT) and labeled with either Cy3-dCTP or Cy5-dCTP by using Superscript II reverse transcriptase (Life Technologies, USA). After hybridization under a standard protocol, data acquisition was performed by using a laser scanner (Agilent, USA), and the image data were analyzed using ImaGene 4.2 (Biodiscovery, Santa Monica, CA, USA).
Platform GPL3498: SBC Rat cDNA Microarray ver2.0
Indicator
Bbs4, Camk2a, Gabbr1, Gabrd, Gfap, Hrh1, Kcna1, Kif5a, Oprl1, Slc6a1, Apbb1, Abca2, Aplp2, App, Htr7, Ntsr2, Ppm1f, Prkar1b, Cdk5, Grm4
Th, Mtap6, Ccr5, Cd74, Cxcl10, Dusp1
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