Antigen specific memory B cells are considered as a homogeneous population. Here, we wanted to isolate antigen-specific memory cells from mice in order to both characterize the response to the immunization regimine and identify high-affinity candidate BCRs for expression. For this, we used single cell RNA sequencing (scRNA-seq) to describe the diversity of anti-fentanyl B cells elicited by a fentanyl-immunization platform and identify the different markers for each subbpopulation. We use this appoach to select the switched memory B cell, study their BCR repertoir and identify the BCR sequences from the most potent memory B cells.

FenAb136, FenAb208, Fcer2a, Cd23, Mzb1, Cd9, Cd38, Ccr4, Fos, Cd40, Irf4, Irf8, Blimp1, Bach2, Bcl6, CDR3