Detail information
ID ENOM00081
Accession GSE214560
Status Public on Oct 04, 2022
Title Morphine PVT TRAPSeq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary
Using an oral self-administration paradigm, we have seen morphine increase neural activity in the Paraventricular Nucleus of the Thalamus (PVT). Morphine binds primarily to Mu Opioid Receptors (MORs), which are highly expressed in the PVT. Translating Ribosome Affinity Purification (TRAP)-Sequencing was conducted on PVT neurons that express MORs
Samples
GSM ID Sample info Characteristics Description
GSM6611783 Male Control 1 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Control drinking water
GSM6611784 Male Control 2 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Control drinking water
GSM6611785 Female Control 5 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Control drinking water
GSM6611786 Female Control 6 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Control drinking water
GSM6611787 Male Morphine 3 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Morphine
GSM6611788 Male Morphine 4 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Morphine
GSM6611789 Female Morphine 7 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Morphine
GSM6611790 Female Morphine 8 tissue: Brain- paraventricular nucleus of thalamus cell type: PVT neurons cell subset: Mu opioid receptor+ genotype: OPRM1cre X EGFP-L10a treatment: Morphine
Platform GPL24247: Illumina NovaSeq 6000 (Mus musculus)
Indicator
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