Detail information
ID ENOM00125
Accession GSE56256
Status Public on Mar 14, 2017
Title Influence of hexafluoro-2-propanol (HFIP) on lipopolysaccharides-induced inflammation in human microvascular endothelial cells of the lung
Organism Homo sapiens
Experiment type Expression profiling by array
Summary
Hexafluoro-2-propanol (HFIP), a water-soluble primary metabolite of the volatile anesthetic sevoflurane, has recently been shown to have favorable properties, improving overall survival in a chronic model of murine peritonitis. The mechanisms of protection provided by HFIP are currently unknown.We therefore performed a gene expression analysis in an in vitro model of endotoxin-induced endothelial cell injury to gain insight into involved molecular mechanisms. Pathway analysis was subsequently performed using the GeneGo Metacore Framework.
Samples
GSM ID Sample info Characteristics Description
GSM1357752 7_HFIP cell type: Human microvascular endothelial cells (HMVEC) exposed to: HFIP 8mmol/L over a time period of 6 hrs Replicate 7 exposed to HFIP 8mmol/L over a time period of 6 hrs
GSM1357753 8_HFIP cell type: Human microvascular endothelial cells (HMVEC) exposed to: HFIP 8mmol/L over a time period of 6 hrs Replicate 8 exposed to HFIP 8mmol/L over a time period of 6 hrs
GSM1357754 9_HFIP cell type: Human microvascular endothelial cells (HMVEC) exposed to: HFIP 8mmol/L over a time period of 6 hrs Replicate 9 exposed to HFIP 8mmol/L over a time period of 6 hrs
GSM1357755 4_LPS cell type: Human microvascular endothelial cells (HMVEC) exposed to: LPS 20mcg/mL over a time period of 6 hrs Replicate 4 exposed to LPS over a time period of 6 hrs
GSM1357756 5_LPS cell type: Human microvascular endothelial cells (HMVEC) exposed to: LPS 20mcg/mL over a time period of 6 hrs Replicate 5 exposed to LPS over a time period of 6 hrs
GSM1357757 6_LPS cell type: Human microvascular endothelial cells (HMVEC) exposed to: LPS 20mcg/mL over a time period of 6 hrs Replicate 6 exposed to LPS over a time period of 6 hrs
GSM1357758 11_LPS-HFIP cell type: Human microvascular endothelial cells (HMVEC) exposed to: LPS 20mcg/mL and HFIP 8mmol/L over a time period of 6 hrs Replicate 11 exposed to LPS and HFIP 8mmol/L over a time period of 6 hrs
GSM1357759 12_LPS-HFIP cell type: Human microvascular endothelial cells (HMVEC) exposed to: LPS 20mcg/mL and HFIP 8mmol/L over a time period of 6 hrs Replicate 12 exposed to LPS and HFIP 8mmol/L over a time period of 6 hrs
GSM1357760 1_PBS cell type: Human microvascular endothelial cells (HMVEC) exposed to: PBS (control) over a time period of 6 hrs Replicate 1 representing the control incubated over a time period of 6 hrs
GSM1357761 2_PBS cell type: Human microvascular endothelial cells (HMVEC) exposed to: PBS (control) over a time period of 6 hrs Replicate 2 representing the control incubated over a time period of 6 hrs
GSM1357762 3_PBS cell type: Human microvascular endothelial cells (HMVEC) exposed to: PBS (control) over a time period of 6 hrs Replicate 3 representing the control incubated over a time period of 6 hrs
Platform GPL13607: Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Indicator
MCP-1, IL-6, CINC-1 Caspase-3, Neutrophil, AST, bilirubin, Creatinine, BUN a-microglobulin, creatinine
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