Detail information
ID ENOM00130
Accession GSE2982
Status Public on Jul 23, 2005
Title Effect of inhaled anesthetic to cultured cortical neurons
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary
Inhaled anesthetics produce many effects and bind to a large number of brain proteins, but it is not yet clear if this is accompanied by widespread changes in gene expression of the biological targets. Such changes in expression might implicate functionally important targets from the large pool of binding targets. Isolated primary cortical neurons were exposed to anesthetics and DNA oligonucleotide microarrays were used to detect and quantify transcriptional changes in neuronal tissue. Keywords: DNA Oligonucleotide Array
Samples
GSM ID Sample info Characteristics Description
GSM61779 Cell Culture Exposure (control1) Cy3 label;Cy5; Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61796 Cell Culture Exposure (control2) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61797 Cell Culture Exposure (control3) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61798 Cell Culture Exposure (1MAC Halo1) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61799 Cell Culture Exposure (1MAC Halo2) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61800 Cell Culture Exposure (1MAC Halo3) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61801 Cell Culture Exposure (3MAC Halo1) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61802 Cell Culture Exposure (3MAC Halo2) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61803 Cell Culture Exposure (3MAC Halo3) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61804 Cell Culture Exposure (3MAC ISO1) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61805 Cell Culture Exposure (3MAC ISO2) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
GSM61806 Cell Culture Exposure (3MAC ISO3) Cy3; Cy5 Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
Platform GPL890: Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version)
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