|
DVID
|
1020022 |
|
Chromosome
|
chr14 |
|
GRCh38 Location
|
94423774, 94423980 |
|
Disease
|
Carcinoma, Hepatocellular |
|
Sample
|
Cell line |
|
Virus Reference Genome
|
X72702 |
|
Target Gene
|
ABHD17B |
Literature Information
|
PubMed PMID
|
31064734
|
|
Year
|
2019 Jun;235-236:39-56 |
|
Journal
|
Cancer genetics |
|
Title
|
Retrotransposon elements among initial sites of hepatitis B virus integration into human genome in the HepG2-NTCP cell infection model. |
|
Author
|
Chauhan R,Shimizu Y,Watashi K,Wakita T,Fukasawa M,Michalak TI |
|
Evidence
|
Integration of hepatitis B virus (HBV) DNA into host's genome is evident in all stages and models of HBV infection. Investigations of the initial virus-host junctions have been just recently initiated since their nature may promote liver oncogenesis immediately following infection. We examined the time-frame and host sites at which HBV integrates in HepG2 cells overexpressing sodium taurocholate co-transporting polypeptide (NTCP) receptor mediating HBV entry. HepG2-NTCP cells were analyzed from 15 min to 13 days post-infection (p.i.). The results showed that except for 15 min p.i., HBV-host integrations were detected at all time points thereafter. At 30 min p.i., virus junctions with retrotransposon SINE and with neuroblastoma breakpoint family member 1 gene were detected. At one-hour p.i., HBV integration with retrotransposon THE-1B-LTR was identified, while virus insertions into proline-rich protein and protein kinase cGMP-dependent type 1 encoding genes were found at 3 h p.i. Fusion with runt-related transcription factor 1 was detected at 24 h p.i. and merges with 9 different genes at 13 day p.i. The data showed that retrotransposon elements are frequent among first-hit sites of HBV insertion. This may suggest a mechanism by which HBV DNA may spread across host's genome from earliest stages of infection.
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HBV VIS Detail Information
