HBV VIS Detail Information

> This page shows VIS [1020220] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.


Site Information
DVID 1020220
Chromosome chr8
GRCh38 Location 35447145
Disease Carcinoma, Hepatocellular  
Sample tumor
Target Gene UNC5D  
Literature Information
PubMed PMID 32570699
Year 2020 Jun 18;11(6):661
Journal Genes
Title Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing.
Author Ishii T,Tamura A,Shibata T,Kuroda K,Kanda T,Sugiyama M,Mizokami M,Moriyama M
Evidence Hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC) worldwide. The integration of HBV genomic DNA into the host genome occurs randomly, early after infection, and is associated with hepatocarcinogenesis in HBV-infected patients. Therefore, it is important to analyze HBV genome integration. We analyzed HBV genome integration in human hepatoma PLC/PRF/5 cells by HBV sequence capture-based next-generation sequencing (NGS) methods. We confirmed the results by using Sanger sequencing methods. We observed that HBV genotype A is integrated into the genome of PLC/PRF/5 cells. HBV sequence capture-based NGS is useful for the analysis of HBV genome integrants and their locations in the human genome. Among the HBV genome integrants, we performed functional analysis and demonstrated the automatic expression of some HBV proteins encoded by HBV integrants from chromosomes 3 and 11 in Huh7 cells transfected with these DNA sequences. HBV sequence capture-based NGS may be a useful tool for the assessment of HBV genome integration into the human genome in clinical samples and suggests new strategies for hepatocarcinogenesis in HBV infection.
Contents
Description
  • Site Information
Detail information of site [1020220]
  • Literature Information
The details of literature that this site is associated with.