HPV VIS Detail Information

> This page shows VIS [5000889] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.

Site Information

DVID	5000889
VISID	TVIS20003814
Chromosome	chr3
GRCh38 Location	189849521
Disease	Squamous Cell Carcinoma of Head and Neck
Sample	Cell line
Virus Reference Genome	NC_001526.4
Target Gene	TP63

Literature Information

PubMed PMID	25082736
Year	2015 Mar 1;136(5):E207-18
Journal	International journal of cancer
Title	Viral load, gene expression and mapping of viral integration sites in HPV16-associated HNSCC cell lines.
Author	Olthof NC,Huebbers CU,Kolligs J,Henfling M,Ramaekers FC,Cornet I,van Lent-Albrechts JA,Stegmann AP,Silling S,Wieland U,Carey TE,Walline HM,Gollin SM,Hoffmann TK,de Winter J,Kremer B,Klussmann JP,Speel EJ
Evidence	HPV-related HNSCC generally have a better prognosis than HPV-negative HNSCC. However, a subgroup of HPV-positive tumors with poor prognosis has been recognized, particularly related to smoking, EGFR overexpression and chromosomal instability. Viral integration into the host genome might contribute to carcinogenesis, as is shown for cervical carcinomas. Therefore, all HPV16-positive HNSCC cell lines currently available have been carefully analyzed for viral and host genome parameters. The viral integration status, viral load, viral gene expression and the presence of aneusomies was evaluated in the cell lines UD-SCC-2, UM-SCC-047, UM-SCC-104, UPCI:SCC090, UPCI:SCC152, UPCI:SCC154 and 93VU147T. HPV integration was examined using FISH, APOT-PCR and DIPS-PCR. Viral load and the expression of the viral genes E2, E6 and E7 were determined via quantitative PCR. All cell lines showed integration-specific staining patterns and signals indicating transcriptional activity using FISH. APOT- and DIPS-PCR identified integration-derived fusion products in six cell lines and only episomal products for UM-SCC-104. Despite the observed differences in viral load and the number of viral integration sites, this did not relate to the identified viral oncogene expression. Furthermore, cell lines exhibited EGFR expression and aneusomy (except UPCI:SCC154). In conclusion, all HPV16-positive HNSCC cell lines showed integrated and/or episomal viral DNA that is transcriptionally active, although viral oncogene expression was independent of viral copy number and the number of viral integration sites. Because these cell lines also contain EGFR expression and aneusomy, which are parameters of poor prognosis, they should be considered suitable model systems for the development of new antiviral therapies.