| 12810357 |
25712 |
Ifng
|
Immunity |
injected at doses of more than 50 microg/kg once a day for 14 days inhibited the ability of inguinal lymph node cells to produce T cell cytokines interleukin 2 and interferon-gamma when the cells were obtained from rats 24 days after immunization and cultured in vitro with CII. |
| 16814689 |
14281 |
Fos
|
Immunity |
Furthermore, BV injection increased Fos expression in tyrosine hydroxylase immunoreactive neurons in the locus caeruleus, and this expression was unaltered by RTX pretreatment. |
| 18023864 |
101097592 |
CNTF
|
Immunity |
CNTF immunopositive neurons in the ND of L(3) segment returned to the sham-operated level at 14 dpo. |
| 18023864 |
101097592 |
CNTF
|
Immunity |
Western blot analysis showed CNTF changes corresponding to those shown in immunohistochemical staining. |
| 18186956 |
12346 |
Car1
|
Immunity |
Three hours after injection, the survival of neuronal cells and the expressions of c-Fos, c-Jun, and glutamate decarboxylase (GAD)-67 in the CA1 and CA3 were determined using immunohistochemistry and Western blotting techniques. |
| 18186956 |
12350 |
Car3
|
Immunity |
Three hours after injection, the survival of neuronal cells and the expressions of c-Fos, c-Jun, and glutamate decarboxylase (GAD)-67 in the CA1 and CA3 were determined using immunohistochemistry and Western blotting techniques. |
| 18186956 |
14281 |
Fos
|
Immunity |
Three hours after injection, the survival of neuronal cells and the expressions of c-Fos, c-Jun, and glutamate decarboxylase (GAD)-67 in the CA1 and CA3 were determined using immunohistochemistry and Western blotting techniques. |
| 18186956 |
16476 |
Jun
|
Immunity |
Three hours after injection, the survival of neuronal cells and the expressions of c-Fos, c-Jun, and glutamate decarboxylase (GAD)-67 in the CA1 and CA3 were determined using immunohistochemistry and Western blotting techniques. |
| 18401708 |
493837 |
BAX
|
Immunity |
This EA-mediated neuroprotection is associated with a decrease in the number of Bax immunoreactive neurons and an increase in the number of Bcl-2 immunoreactive neurons. |
| 18401708 |
493934 |
BCL2
|
Immunity |
This EA-mediated neuroprotection is associated with a decrease in the number of Bax immunoreactive neurons and an increase in the number of Bcl-2 immunoreactive neurons. |
| 19056464 |
13162 |
Slc6a3
|
Immunity |
Acupuncture inhibited decreases in the immunoreactivities of tyrosine hydroxylase (TH) and dopamine transporter (DAT) that occurred as a result of MPTP neurotoxicity. |
| 19390163 |
12097 |
Bglap2
|
Immunity |
The serum testosterone and osteocalcin (OC) levels were determined by radioimmunoassay. |
| 19514190 |
15944 |
Irgm1
|
Immunity |
CONCLUSION: The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging. |
| 19514190 |
18022 |
Nfe2
|
Immunity |
CONCLUSION: The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging. |
| 19514190 |
22608 |
Ybx1
|
Immunity |
CONCLUSION: The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging. |
| 19607689 |
21333 |
Tac1
|
Immunity |
Nine days after inoculation, immunohistochemistry was performed using antibodies against substance P, in sections of spinal cord dorsal horn of mice. |
| 21625423 |
21823 |
Th
|
Immunity |
Here we assessed whether EA stimulation could rescue DA neurons in the SNpc from MPTP toxicity by TH immunohistochemistry. |
| 21625423 |
21823 |
Th
|
Immunity |
However, TH immunoreactivity could be rescued by 100 Hz EA stimulation (p<0.05 vs. |
| 21625423 |
21823 |
Th
|
Immunity |
Consistent with the TH immunohistochemistry results, 0 Hz EA stimulation did not affect the concentrations of DA, DOPAC and HVA in the striatum of the MPTP treated mice. |
| 21796340 |
18976 |
Pomc
|
Immunity |
Plasma beta-endorphin was measured 40 min after the acupuncture treatment by immunoassay. |
| 22020787 |
18126 |
Nos2
|
Immunity |
The authors measured the degree of positive reaction to substance P, STAT6, nuclear factor kappa B (NFkappaB), and iNOS via immunohistochemical staining of the nasal mucosa. |
| 22020787 |
18033 |
Nfkb1
|
Immunity |
The authors measured the degree of positive reaction to substance P, STAT6, nuclear factor kappa B (NFkappaB), and iNOS via immunohistochemical staining of the nasal mucosa. |
| 22020787 |
20852 |
Stat6
|
Immunity |
The authors measured the degree of positive reaction to substance P, STAT6, nuclear factor kappa B (NFkappaB), and iNOS via immunohistochemical staining of the nasal mucosa. |
| 22020787 |
21333 |
Tac1
|
Immunity |
The authors measured the degree of positive reaction to substance P, STAT6, nuclear factor kappa B (NFkappaB), and iNOS via immunohistochemical staining of the nasal mucosa. |
| 22132113 |
19049 |
Ppp1r1b
|
Immunity |
Therefore, we examined MPTP-induced postsynaptic changes using immunohistochemical staining for phospho-DARPP-32 at Thr34. |
| 24321617 |
14282 |
Fosb
|
Immunity |
Next, we investigated the abnormal protein activation of FosB via western blot and immunofluorescence analysis. |
| 24524846 |
26417 |
Mapk3
|
Immunity |
To investigate the location of ERK activation in the skin layer, a histologic analysis with immunohistochemistry and immunofluorescence was performed. |
| 24524846 |
26413 |
Mapk1
|
Immunity |
To investigate the location of ERK activation in the skin layer, a histologic analysis with immunohistochemistry and immunofluorescence was performed. |
| 24524846 |
26417 |
Mapk3
|
Immunity |
To investigate the type of cell undergoing ERK activation, the tissue was costained for phospho-ERK, a fibroblast marker, and a keratinocyte marker, and then visualized by immunofluorescence (Fig 6). |
| 24524846 |
26413 |
Mapk1
|
Immunity |
To investigate the type of cell undergoing ERK activation, the tissue was costained for phospho-ERK, a fibroblast marker, and a keratinocyte marker, and then visualized by immunofluorescence (Fig 6). |
| 25558226 |
101087287 |
PDGFA
|
Immunity |
PDGF positive immunoreactive products were present mainly in the cytoplasm, and in the partial fibers, without pronounced satellite cell staining (Figure 1, Table 1). |
| 25558226 |
100135774 |
PDGFB
|
Immunity |
PDGF positive immunoreactive products were present mainly in the cytoplasm, and in the partial fibers, without pronounced satellite cell staining (Figure 1, Table 1). |
| 25558226 |
101088742 |
PDGFC
|
Immunity |
PDGF positive immunoreactive products were present mainly in the cytoplasm, and in the partial fibers, without pronounced satellite cell staining (Figure 1, Table 1). |
| 25558226 |
101084984 |
PDGFD
|
Immunity |
PDGF positive immunoreactive products were present mainly in the cytoplasm, and in the partial fibers, without pronounced satellite cell staining (Figure 1, Table 1). |
| 25558226 |
101087287 |
PDGFA
|
Immunity |
Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7th day following injury. |
| 25558226 |
100135774 |
PDGFB
|
Immunity |
Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7th day following injury. |
| 25558226 |
101088742 |
PDGFC
|
Immunity |
Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7th day following injury. |
| 25558226 |
101084984 |
PDGFD
|
Immunity |
Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7th day following injury. |
| 25968478 |
14281 |
Fos
|
Immunity |
With immunohistochemical staining method, we observed c-fos expression, distribution and changes after acupuncture on sensory pathway, including colorectum, spinal dorsal horn and different regions of brain center in the model with colorectal distension stimulation, and evaluated the acupuncture effect on brain-gut axis. |
| 25983633 |
14012 |
Mpzl2
|
Immunity |
Mpzl2 expressed in choroid plexus cells may regulate the permeability of the blood-cerebrospinal fluid (CSF) barrier, suggesting a novel mechanism of CNS immune surveillance regulation. |
| 25983633 |
21823 |
Th
|
Immunity |
Immunohistochemical analyses using Western blots confirmed that the decrease in TH levels in the striatal and SNpc regions induced by MPTP intoxication was significantly inhibited by acupuncture at acupoints (MPTP-A) but not at non-acupoints (MPTP-NA; Figure 2). |
| 26016857 |
20617 |
Snca
|
Immunity |
Western blots also showed that the level of exogenous alpha-syn was gradually increased in the midbrain and striatum over different time points (1 month, 2 month, and symptomatic time) in A53T mice, while alpha-syn immunoreactivity was absent in these regions in nTg control mice (Fig. |
| 26016857 |
14580 |
Gfap
|
Immunity |
Immunofluorescent staining revealed a larger number of GFAP-positive astrocytes in the SN of A53T mice (Fig. |
| 26016857 |
14580 |
Gfap
|
Immunity |
EA (100 Hz) decreased the number of GFAP-immunoreactive astrocytes (Fig. |
| 26016857 |
14580 |
Gfap
|
Immunity |
Similarly, in immunoblot assays, EA effectively alleviated the excessive expression of GFAP in the SN of A53T mice (Fig. |
| 26016857 |
114737 |
Iba1
|
Immunity |
Activated microglia was observed in the SN of A53T mice, as evidenced by increased Iba-1 immunoreactivity and by the appearance of microglia clusters (Fig. |
| 26016857 |
114737 |
Iba1
|
Immunity |
EA stimulation effectively reduced the intensity of Iba-1 immunostaining and the clusters of activated microglia. |
| 26016857 |
52897 |
Rbfox3
|
Immunity |
In addition, changes of neurons in the spinal cord were investigated by the immunostaining of NeuN. |
| 26991319 |
16784 |
Lamp2
|
Immunity |
Double immunofluorescence examination was performed to locate lysosome with an anti-LAMP2 antibody and DA neurons with an anti-tyrosine hydroxylase (anti-TH) antibody, which further confirms the appearance of more lysosomes in SNpc DA neurons after the acupuncture treatment (Fig. |
| 27381504 |
108058 |
Camk2d
|
Immunity |
Expression of NMDAR subunits, calmodulin-dependent protein kinase II (CaMKII), cyclic AMP response element binding protein (pCREB) and their corresponding phospho-activated forms were measured by Western blotting and immunohistochemistry. |
| 27381504 |
14810 |
Grin1
|
Immunity |
Expression of NMDAR subunits, calmodulin-dependent protein kinase II (CaMKII), cyclic AMP response element binding protein (pCREB) and their corresponding phospho-activated forms were measured by Western blotting and immunohistochemistry. |
| 27381504 |
14810 |
Grin1
|
Immunity |
By contrast, there were no differences in NR1 immunoreactivity between groups (figure 2B). |
| 27966183 |
|
|
Immunity |
In this study, we aimed to explore the immunomodulatory mechanism of EA intervention in a mouse model of ovalbumin (OVA)-induced DTH. |
| 28450287 |
14683 |
Gnas
|
Immunity |
The findings of the G protein activation assays via immunoprecipitation and Western blots were that the physiologically coupled activation rate (PCAR) and maximal coupled activation rate (MCAR) of Galphas and Galphai were decreased in the cortex of SAMP8 vs SAMR1 mice. |
| 28806763 |
78240 |
Cst11
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
69362 |
Cst12
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
319579 |
Defb20
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
654459 |
Defb25
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
545475 |
Defb28
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
432867 |
Defb48
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
14780 |
Gpx5
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
78076 |
Lcn8
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
77704 |
Lcn9
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28806763 |
20850 |
Stat5a
|
Immunity |
Gene ontology (GO) annotation indicated that the up-regulated genes in the hypothalamus of the Stat5NKO mice were mainly expressed in the extracellular region, basement membrane, plasma lipoprotein particle, and cytosol (Fig 2A); and these genes were significantly enriched in 140 biological processes, which were functionally clustered to extracellular matrix organization, response to hormone and insulin stimulus, lipid and saccharide metabolic process, fat cell differentiation, and positive regulation of adaptive and innate immune responses. |
| 28806763 |
20850 |
Stat5a
|
Immunity |
From the above analysis, we observed that a large number of genes and functional pathways involved in lipid metabolism, adipogenesis, insulin signaling, and immune system were dependent on the STAT5 molecule in the CNS. |
| 28806763 |
20850 |
Stat5a
|
Immunity |
Among these genes (Fig 4E), FPKM values of 7 genes, which are associated with immune system and the response to hormone (AY761185, Cst11, Defb20, Defb28, Defb48, Lcn8, Lcn9) were decreased to zero by the deletion of central Stat5, whereas EA treatment reversed their expression levels, and the other 13 genes, such as Gpx5, 9230104L09Rik, Cst12, and Defb25, appeared in the top 40 down-regulated gene list of the Stat5NKO mice (S7 Table). |
| 28837077 |
24224 |
Bcl2
|
Immunity |
These findings indicate that MPP+-induced dopaminergic neuronal death (tyrosine hydroxylase ) in rat SN involves neuronal apoptosis (Bcl-2 ), which is consistent with our immunohistochemical analyses of tyrosine hydroxylase, as shown in the upper part of Figure 3A. |
| 29981376 |
12846 |
Comt
|
Immunity |
Consistent with clinical syndromes, we previously demonstrated that COMT inhibition in rodents produces persistent pain and heightened immune responses. |
| 29981376 |
14580 |
Gfap
|
Immunity |
Immunohistochemical analysis of spinal phosphorylated p38 mitogen-activated kinase (p-p38 MAPK), a marker of inflammation, and glial fibrillary acidic protein (GFAP), a marker of astrogliosis, was performed on day 13. |
| 29981376 |
14580 |
Gfap
|
Immunity |
To evaluate changes in the activation of astrocytes in our mouse model of COPCs, we performed immunostaining of spinal cord sections collected on day 13 using an anti-GFAP antibody as a marker of astrogliosis. |
| 29981376 |
26416 |
Mapk14
|
Immunity |
To evaluate changes in the activation (phosphorylation; p) of p38, we performed immunostaining in spinal cord sections collected on day 13 using an anti-p-p38 antibody. |
| 30105072 |
66824 |
Pycard
|
Immunity |
DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. |
| 30105072 |
12362 |
Casp1
|
Immunity |
DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. |
| 30105072 |
16176 |
Il1b
|
Immunity |
DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. |
| 30105072 |
16176 |
Il1b
|
Immunity |
Expression of IL-1beta and NLRP3 Related Proteins in Hippocampus: Seeing from Immunohistochemistry |
| 30105072 |
16176 |
Il1b
|
Immunity |
In the immunohistochemical results (Figure 1), the number of positive cells expressing IL-1beta protein in hippocampus of mice in N group was low, and occasional positive cells were also lighter in staining. |
| 30105072 |
216799 |
Nlrp3
|
Immunity |
DU20, DU26, and EX-HN3 were selected as the acupuncture points, and after a 15-day treatment of electroacupuncture, we used immunohistochemistry and Western blotting to examine the expression of IL-1beta and NLRP3, ASC, and Caspase-1 in the hippocampus of the AD animal model. |
| 30105072 |
216799 |
Nlrp3
|
Immunity |
Expression of IL-1beta and NLRP3 Related Proteins in Hippocampus: Seeing from Immunohistochemistry |
| 30151295 |
14580 |
Gfap
|
Immunity |
The expression levels of glial fibrillary acidic protein (GFAP) in the hippocampus were determined by using western blot and immunohistochemistry analyses. |
| 30151295 |
14580 |
Gfap
|
Immunity |
The immunoreactivity of GFAP in the CA3 region of the hippocampus is shown in Fig. |
| 30151295 |
14580 |
Gfap
|
Immunity |
Slides from the control mice displayed moderate GFAP immunostaining in the CA3 region of the hippocampus (Figs. |
| 30151295 |
14580 |
Gfap
|
Immunity |
Notably, slides from stressed mice treated with scolopendra pharmacopuncture also displayed a moderate level of GFAP immunostaining, resembling that seen in the slides of the control mice, in the CA3 region of the hippocampus (Figs. |
| 30505267 |
11651 |
Akt1
|
Immunity |
To investigate whether AP and EA affect phosphorylation levels of Akt, we performed immunoblot analysis at 3, 7, 14 and 28 days after SCI on spinal cord tissue containing the injury site at the center and 0.5 cm tissue each rostral and caudal to the injury site (Figure 1). |
| 30505267 |
26417 |
Mapk3
|
Immunity |
To investigate whether AP and EA affect p-Erk levels, we performed immunoblot analysis at 3, 7, 14 and 28 days after SCI on spinal cord tissue (Figure 2). |
| 30505267 |
26413 |
Mapk1
|
Immunity |
To investigate whether AP and EA affect p-Erk levels, we performed immunoblot analysis at 3, 7, 14 and 28 days after SCI on spinal cord tissue (Figure 2). |
| 30505267 |
17196 |
Mbp
|
Immunity |
To evaluate whether AP and EA affect expression of MBP after SCI, we performed immunofluorescence staining and immunoblot analysis. |
| 30505267 |
22059 |
Trp53
|
Immunity |
To investigate whether AP and EA affect p53 expression, we performed immunoblot analysis at 3, 7, 14 and 28 days after SCI (Figure 5). |
| 30505267 |
19211 |
Pten
|
Immunity |
To investigate whether AP and EA affect the expression of PTEN, we performed immunoblot analysis at 3, 7, 14 and 28 days after SCI (Figure 4). |
| 30510442 |
12802 |
Cnr2
|
Immunity |
The expression of CB2 receptor and IL-1beta were quantified by using immunofluorescence labeling. |
| 30510442 |
16176 |
Il1b
|
Immunity |
The expression of CB2 receptor and IL-1beta were quantified by using immunofluorescence labeling. |
| 30726543 |
12367 |
Casp3
|
Immunity |
The apoptosis factor caspase-3 was identified through an enzyme-linked immunosorbent assay (ELISA). |
| 31192694 |
56461 |
Kcnip3
|
Immunity |
After EA treatment for 3 days, immune response, natural killer (NK) cell toxicity and the expression of cytokines and DREAM/NF-kappaB were assessed. |
| 31192694 |
18033 |
Nfkb1
|
Immunity |
Electroacupuncture regulates the DREAM/NF-kappaB signalling pathway and ameliorates cyclophosphamide-induced immunosuppression in mice. |
| 31192694 |
18033 |
Nfkb1
|
Immunity |
OBJECTIVE: To examine whether electroacupuncture (EA) promotes the immune response by regulating the downstream regulatory element antagonist modulator / nuclear factor kappa B (DREAM/NF-kappaB) signalling pathway in a mouse model of cyclophosphamide (CP)-induced immunosuppression, and determine the most effective frequency. |
| 31192694 |
56461 |
Kcnip3
|
Immunity |
Electroacupuncture regulates the DREAM/NF-kappaB signalling pathway and ameliorates cyclophosphamide-induced immunosuppression in mice. |
| 31192694 |
56461 |
Kcnip3
|
Immunity |
OBJECTIVE: To examine whether electroacupuncture (EA) promotes the immune response by regulating the downstream regulatory element antagonist modulator / nuclear factor kappa B (DREAM/NF-kappaB) signalling pathway in a mouse model of cyclophosphamide (CP)-induced immunosuppression, and determine the most effective frequency. |
| 31192694 |
15978 |
Ifng
|
Immunity |
Additionally, 2/100 Hz EA treatment increased interleukin (IL)-2, IL-6, IL-12, tumour necrosis factor-alpha and interferon-gamma levels and decreased IL-10 levels in CP-induced immunosuppressed mice. |
| 31192694 |
16153 |
Il10
|
Immunity |
Additionally, 2/100 Hz EA treatment increased interleukin (IL)-2, IL-6, IL-12, tumour necrosis factor-alpha and interferon-gamma levels and decreased IL-10 levels in CP-induced immunosuppressed mice. |
| 31192694 |
16183 |
Il2
|
Immunity |
Additionally, 2/100 Hz EA treatment increased interleukin (IL)-2, IL-6, IL-12, tumour necrosis factor-alpha and interferon-gamma levels and decreased IL-10 levels in CP-induced immunosuppressed mice. |
| 31192694 |
16193 |
Il6
|
Immunity |
Additionally, 2/100 Hz EA treatment increased interleukin (IL)-2, IL-6, IL-12, tumour necrosis factor-alpha and interferon-gamma levels and decreased IL-10 levels in CP-induced immunosuppressed mice. |
| 31192694 |
18033 |
Nfkb1
|
Immunity |
After EA treatment for 3 days, immune response, natural killer (NK) cell toxicity and the expression of cytokines and DREAM/NF-kappaB were assessed. |
| 31447579 |
26416 |
Mapk14
|
Immunity |
Phospho-p38 MAPK nuclear translocation in spinal dorsal horn was examined using immunohistochemical staining and confocal microscopy. |
| 31447579 |
26416 |
Mapk14
|
Immunity |
We observed a significantly high co-localization of phospho-p38 MAPK immunoreactivity in the nulei of non-responders (red and white dots indicated by yellow arrows in the cyan nuclei) compared to high- responders (Figure 3C, D). |
| 31447579 |
26416 |
Mapk14
|
Immunity |
High-responders did not show significant p38 MAPK phosphorylation upon Western blotting (Figure 3A and B) or upon immunofluorescent co-localization in the cytoplasm and/or nuclear translocation (Figure 3C, D). |
| 31600836 |
14281 |
Fos
|
Immunity |
Thus, we mapped P6 specific neural activation by MA in the forebrain using the Fos-CreER; Ai9 mice model, which allows for enhanced sensitivity and efficiency compared to conventional immunohistochemical staining. |
| 31941349 |
193034 |
Trpv1
|
Immunity |
TRPV1 and pERK expression levels were measured using Western blotting and immunohistochemistry. |
| 32796318 |
52897 |
Rbfox3
|
Immunity |
7B) were costained with NeuN for double-immunofluorescence analysis. |
| 32989889 |
101409728 |
LDH
|
Immunity |
Concentrations of LDH and NAGase were determined using commercial enzyme-linked immunosorbent assays. |
| 33607237 |
16846 |
Lep
|
Immunity |
Then, lipidomics was applied to investigate the effects of acupuncture on lipid metabolism and analyze leptin signals in the brain and changes of immune markers. |
| 33607237 |
16846 |
Lep
|
Immunity |
In conclusion, these results show that AP acupuncture treatment effectively alleviated the depression-like behavior, affected immune responses, and altered hepatic lipid metabolism through the attenuation of leptin insensitivity. |
| 34562596 |
11450 |
Adipoq
|
Immunity |
The immunofluorescence staining analysis showed that EA increased the APN accumulation in spinal cord through the blood circulation. |
| 35138669 |
56318 |
Acp3
|
Immunity |
Specifically, we used a luminescence assay, quantitative reverse transcriptase-polymerase chain reaction, western blotting, immunohistochemistry and nociceptive-related behavioural changes to gather data, and we tested for effects after PAP expression was inhibited with an adeno-associated virus (AAV). |
| 35138669 |
56318 |
Acp3
|
Immunity |
Moreover, membrane PAP degradation was investigated in cultured DRG neurons and the inhibitory effects of EA on this degradation were assessed using immunoprecipitation. |
| 35178106 |
11651 |
Akt1
|
Immunity |
Taken together, our data corroborate the effective potency of CIAA in the treatment of HCC by and inhibiting immune escape and deactivating the AKT pathway. |
| 35484628 |
15978 |
Ifng
|
Immunity |
Gene ontology (GO) annotation indicated that the coregulated genes in the heart were mainly expressed in biological processes of the inflammatory response, immune system processes, innate immune responses, cell adhesion, positive regulation of angiogenesis, adaptive immune responses, positive regulation of cell division, and positive regulation of interferon-gamma production (Fig. |
| 35484628 |
21926 |
Tnf
|
Immunity |
Immunohistochemistry also indicated that acupuncture reduced TNF-alpha expression, which was elevated in MI cardiac tissue (Fig. |
| 35656078 |
114737 |
Iba1
|
Immunity |
Immunofluorescence staining also confirmed elevated expression of the glial cell marker Iba1 in the DRG following CIP induction, which was attenuated by AI treatment and Trpv1 gene deletion (Figure 6B). |
| 35656078 |
114737 |
Iba1
|
Immunity |
In accord with western blotting and other immunofluorescence results, TRPV1 immunoreactivity (Figure 8A) and Iba1 immunoreactivity (Figure 8B) were elevated concomitantly by CIP induction. |
| 35656078 |
193034 |
Trpv1
|
Immunity |
Immunofluorescence images of the DRG revealed changes in TRPV1 immunoexpression that paralleled those measured by western blotting, with elevation following CIP induction compared with sham control mice and markedly lower expression levels in AI and Trpv1-/- groups (Figure 6A). |
| 35656078 |
193034 |
Trpv1
|
Immunity |
Immunofluorescence staining also confirmed elevated expression of the glial cell marker Iba1 in the DRG following CIP induction, which was attenuated by AI treatment and Trpv1 gene deletion (Figure 6B). |
| 35656078 |
193034 |
Trpv1
|
Immunity |
In accord with western blotting and other immunofluorescence results, TRPV1 immunoreactivity (Figure 8A) and Iba1 immunoreactivity (Figure 8B) were elevated concomitantly by CIP induction. |
| 35882809 |
21366 |
Slc6a6
|
Immunity |
Immunohistochemical staining confirmed that the expression of TauT and taurine in the cerebellum and hippocampus increased. |
| 37128191 |
13193 |
Dcx
|
Immunity |
The neurogenesis-promoting effect of EA was evaluated based on the number of BrdU-positive cells and BrdU/DCX double-immunoreactive cells. |
| 37128191 |
13193 |
Dcx
|
Immunity |
The number of BrdU/DCX double-immunoreactive cells was significantly lower in the MPTP, MPTP + EA, and MPTP + sham groups than in the control group (p < 0.001, p < 0.01, and p < 0.01, respectively), while this number was significantly higher in the MPTP + EA group than in the MPTP group (p < 0.05; Fig. |
| 37128191 |
21823 |
Th
|
Immunity |
Western blotting confirmed TH expression in the striatum, and a similar trend was observed on immunohistochemical examination (Fig. |
| 37635258 |
12914 |
Crebbp
|
Immunity |
6 genes are related to tumor immunity, such as histone acetyltransferase P300 (EP300), CREB-binding protein (CREBBP), Catenin 1 (CTNNB1), and the proto-oncogene (JUN). |
| 37635258 |
12387 |
Ctnnb1
|
Immunity |
6 genes are related to tumor immunity, such as histone acetyltransferase P300 (EP300), CREB-binding protein (CREBBP), Catenin 1 (CTNNB1), and the proto-oncogene (JUN). |
| 37635258 |
13649 |
Egfr
|
Immunity |
Among the 20 interacting genes screened, 9 genes were related to inflammation and immunomodulatory, such as heat shock protein 90kDaalpha (HSP90AA1), Growth factor receptor binding protein 2 (GRB2), and Epidermal growth factor receptor (EGFR). |
| 37635258 |
328572 |
Ep300
|
Immunity |
6 genes are related to tumor immunity, such as histone acetyltransferase P300 (EP300), CREB-binding protein (CREBBP), Catenin 1 (CTNNB1), and the proto-oncogene (JUN). |
| 37635258 |
14784 |
Grb2
|
Immunity |
Among the 20 interacting genes screened, 9 genes were related to inflammation and immunomodulatory, such as heat shock protein 90kDaalpha (HSP90AA1), Growth factor receptor binding protein 2 (GRB2), and Epidermal growth factor receptor (EGFR). |
| 37635258 |
15519 |
Hsp90aa1
|
Immunity |
Among the 20 interacting genes screened, 9 genes were related to inflammation and immunomodulatory, such as heat shock protein 90kDaalpha (HSP90AA1), Growth factor receptor binding protein 2 (GRB2), and Epidermal growth factor receptor (EGFR). |
| 37635258 |
21926 |
Tnf
|
Immunity |
There were also signaling pathways closely related to inflammation and immune regulation, such as MAPK signaling pathway, TNF signaling pathway, JAK-STAT signaling pathway, T cell receptor signaling pathway (Fig. |
| 37992796 |
11820 |
App
|
Immunity |
Hippocampal Abeta expression was detected by immunohistochemistry and ELISA. |
| 37992796 |
11820 |
App
|
Immunity |
Immunofluorescence method was applied to examine the expression of TFEB in CA1 region of the hippocampus and the co-localization of CTSD or LAMP1 with Abeta. |
| 37992796 |
13033 |
Ctsd
|
Immunity |
Immunofluorescence method was applied to examine the expression of TFEB in CA1 region of the hippocampus and the co-localization of CTSD or LAMP1 with Abeta. |
| 37992796 |
16783 |
Lamp1
|
Immunity |
Immunofluorescence method was applied to examine the expression of TFEB in CA1 region of the hippocampus and the co-localization of CTSD or LAMP1 with Abeta. |
| 37992796 |
21425 |
Tfeb
|
Immunity |
Immunofluorescence method was applied to examine the expression of TFEB in CA1 region of the hippocampus and the co-localization of CTSD or LAMP1 with Abeta. |
| 38189854 |
20617 |
Snca
|
Immunity |
Western blotting was used to detect the expression of related proteins in mouse pathological samples; TUNEL measured neuronal apoptosis levels; Nissl staining observed neuronal damage; immunofluorescence and immunohistochemistry were used to detect the expression of Iba-1, TH, and alpha-syn in substantia nigra denser (SN). |
| 38189854 |
114737 |
Iba1
|
Immunity |
Western blotting was used to detect the expression of related proteins in mouse pathological samples; TUNEL measured neuronal apoptosis levels; Nissl staining observed neuronal damage; immunofluorescence and immunohistochemistry were used to detect the expression of Iba-1, TH, and alpha-syn in substantia nigra denser (SN). |
| 38189854 |
21823 |
Th
|
Immunity |
Western blotting was used to detect the expression of related proteins in mouse pathological samples; TUNEL measured neuronal apoptosis levels; Nissl staining observed neuronal damage; immunofluorescence and immunohistochemistry were used to detect the expression of Iba-1, TH, and alpha-syn in substantia nigra denser (SN). |
| 22550540 |
314322 |
Fos
|
Immunity |
Behavioral responses were recorded using a video camera and c-Fos immunohistochemistry was performed thereafter. |
| 22550540 |
314322 |
Fos
|
Immunity |
3.2. c-Fos Immunohistochemistry |
| 22550540 |
314322 |
Fos
|
Immunity |
In order to compare the level of c-Fos immunoreactivity in the Formalin group Group 1 with pain-free animals, a normal naive animal group Group 0 was added to this study. |
| 22553490 |
25737 |
Pcna
|
Immunity |
Sperm motility and production, morphology of the germinal epithelium by Johnsen's scoring, germ cell apoptosis by TUNEL staining, proliferation by proliferating cell nuclear antigen (PCNA) staining, as well as serum testosterone and inhibin B levels by immunoassays were evaluated on day 0, 1, 9, 25, 37, 46, 56 and 79. |
| 23304201 |
29583 |
Pecam1
|
Immunity |
Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31, alpha-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels, alpha-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. |
| 23304201 |
29583 |
Pecam1
|
Immunity |
To investigate whether acupuncture treatment promotes angiogenesis, angiogenesis factor, and endothelial cell of newly generated cells such as VEGF and CD-31 were analyzed using the ELISA and immunostaining. |
| 23304201 |
25737 |
Pcna
|
Immunity |
Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31, alpha-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels, alpha-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. |
| 23304201 |
25737 |
Pcna
|
Immunity |
We examine whether acupuncture could promote the induction of extracellular matrix remodeling by immunostaining for PCNA and several phenotype markers including alpha-SMA and collagen type I in the wound area. |
| 23304201 |
83785 |
Vegfa
|
Immunity |
To investigate whether acupuncture treatment promotes angiogenesis, angiogenesis factor, and endothelial cell of newly generated cells such as VEGF and CD-31 were analyzed using the ELISA and immunostaining. |
| 23349861 |
25139 |
Slc2a4
|
Immunity |
Low-frequency electrical stimulation modified gene expression (decreased Tbc1d1 in soleus, increased Nr4a3 in mesenteric fat) and protein expression (increased pAS160/AS160, Nr4a3 and decreased GLUT4) by western blot and increased GLUT4 expression by immunohistochemistry in soleus muscle; glucose clearance during oral glucose tolerance tests was unaffected. |
| 23349861 |
58853 |
Nr4a3
|
Immunity |
Low-frequency electrical stimulation modified gene expression (decreased Tbc1d1 in soleus, increased Nr4a3 in mesenteric fat) and protein expression (increased pAS160/AS160, Nr4a3 and decreased GLUT4) by western blot and increased GLUT4 expression by immunohistochemistry in soleus muscle; glucose clearance during oral glucose tolerance tests was unaffected. |
| 23349861 |
360937 |
Tbc1d1
|
Immunity |
Low-frequency electrical stimulation modified gene expression (decreased Tbc1d1 in soleus, increased Nr4a3 in mesenteric fat) and protein expression (increased pAS160/AS160, Nr4a3 and decreased GLUT4) by western blot and increased GLUT4 expression by immunohistochemistry in soleus muscle; glucose clearance during oral glucose tolerance tests was unaffected. |
| 23542071 |
29373 |
Bmp2
|
Immunity |
Haematoxylin and eosin (H&E) staining was used to measure total cell count and immunohistochemical staining to assess the increase in the bone morphogenetic protein 2 (BMP-2)-positive cells. |
| 23573158 |
29256 |
Oprl1
|
Immunity |
The expression of OFQ and of its receptor in L6-S1 spinal dorsal horn was also tested by ISH, immunohistochemistry and ELISA. |
| 23751198 |
58812 |
Apln
|
Immunity |
The expression of both apelin and the APJ receptor in the RVLM neurons was examined by immunohistochemical staining and Western blots. |
| 24192526 |
81633 |
Acta2
|
Immunity |
PAECs were characterized by morphological activity and by immunostaining for von Willebrand factor, CD31 and CD34, but not for alpha-smooth muscle actin, smooth muscle myosin heavy chain or CD90/Thy-1. |
| 24192526 |
24582 |
Myh11
|
Immunity |
PAECs were characterized by morphological activity and by immunostaining for von Willebrand factor, CD31 and CD34, but not for alpha-smooth muscle actin, smooth muscle myosin heavy chain or CD90/Thy-1. |
| 24192526 |
24832 |
Thy1
|
Immunity |
PAECs were characterized by morphological activity and by immunostaining for von Willebrand factor, CD31 and CD34, but not for alpha-smooth muscle actin, smooth muscle myosin heavy chain or CD90/Thy-1. |
| 24334277 |
63868 |
Hspd1
|
Immunity |
Heat shock protein 60, a protein released by cells undergoing necrotic cell death, may activate innate immune cells through a TLR4-dependent mechanism. |
| 24334277 |
29260 |
Tlr4
|
Immunity |
Heat shock protein 60, a protein released by cells undergoing necrotic cell death, may activate innate immune cells through a TLR4-dependent mechanism. |
| 24496685 |
54258 |
Cxcr1
|
Immunity |
The expression of CXCR1 and CXCR2 in rat endometrium was detected by immunohistochemistry, Western blotting and real-time PCR. |
| 24496685 |
29385 |
Cxcr2
|
Immunity |
The expression of CXCR1 and CXCR2 in rat endometrium was detected by immunohistochemistry, Western blotting and real-time PCR. |
| 24527041 |
25085 |
Th
|
Immunity |
TH-like immunoreactivity was also analyzed in the cell bodies of major noradrenergic regions, including the LC (Figure 9(a)). |
| 24527041 |
25085 |
Th
|
Immunity |
In the brains of the MOR group, the number of TH immunoreactive neurons in the LC was decreased by 64.52%. |
| 24527041 |
25085 |
Th
|
Immunity |
Analysis of the numbers of TH-immunoreactive neurons values revealed that rats repeatedly exposed to morphine exhibit a significant decrease of TH expression compared to the SAL group (P < 0.01; Figure 9(c)). |
| 24527041 |
25085 |
Th
|
Immunity |
The number of TH-immunoreactive neurons was significantly increased in the central adrenergic regions of the MOR-SP group compared to the MOR group (P < 0.05). |
| 24527041 |
25085 |
Th
|
Immunity |
This finding indicates that the increase in the number of CRF-immunoreactivity and decrease in the number of TH-immunoreactivity induced by withdrawal were significantly restored by acupuncture stimulation to the SP6 acupoint. |
| 24610411 |
108348108 |
Hspa1b
|
Immunity |
Blood was sampled to detect the HSP70 and TNF-alpha content by enzyme linked immunosorbent assay. |
| 24610411 |
24835 |
Tnf
|
Immunity |
Blood was sampled to detect the HSP70 and TNF-alpha content by enzyme linked immunosorbent assay. |
| 24722278 |
81633 |
Acta2
|
Immunity |
To verify that angiogenesis is a possible mechanism in the EA-induced myocardioprotective effect; angiogenic responses were examined by immunofluorescence staining of CD34, alpha-SMA and VEGF. |
| 24722278 |
305081 |
Cd34
|
Immunity |
To verify that angiogenesis is a possible mechanism in the EA-induced myocardioprotective effect; angiogenic responses were examined by immunofluorescence staining of CD34, alpha-SMA and VEGF. |
| 24722278 |
83785 |
Vegfa
|
Immunity |
To verify that angiogenesis is a possible mechanism in the EA-induced myocardioprotective effect; angiogenic responses were examined by immunofluorescence staining of CD34, alpha-SMA and VEGF. |
| 24757373 |
314322 |
Fos
|
Immunity |
c-Fos immunohistochemistry |
| 24757373 |
314322 |
Fos
|
Immunity |
Fos-like immunoreactivity was examined by immunohistochemistry in the rat spinal cord. |
| 24789671 |
29613 |
Ntrk3
|
Immunity |
The expression of TrkC was also confirmed by Western blot and immunohistochemistry. |
| 24828425 |
25293 |
Aqp4
|
Immunity |
To evaluate the astrocytic and vascular localization of MMP2 and AQP4 in the ischemic penumbra of CIRI model rats, immunofluorescence analysis was employed using specific primary antibodies against MMP2, AQP4, GFAP (to label astrocytes), and CD34 (to label the endothelial cell components of blood vessels). |
| 24828425 |
25293 |
Aqp4
|
Immunity |
Next, we explored the hypothesis that acupuncture and electroacupuncture can regulate MMP2, AQP4, and AQP9 expression in the ischemic penumbra and the core zone by performing an immunohistochemical analysis of target protein expression, followed by semi-quantitative measurement of the integrated optical density of MMP2/AQP4/APQ9 labeling. |
| 24828425 |
25293 |
Aqp4
|
Immunity |
The Western blotting data (Figure 7) verified the immunohistochemical findings (Figure 6), in that the protein expression levels of MMP2, AQP4, and AQP9 were all significantly higher in group M than in the other four groups (P<0.05). |
| 24828425 |
65054 |
Aqp9
|
Immunity |
Next, we explored the hypothesis that acupuncture and electroacupuncture can regulate MMP2, AQP4, and AQP9 expression in the ischemic penumbra and the core zone by performing an immunohistochemical analysis of target protein expression, followed by semi-quantitative measurement of the integrated optical density of MMP2/AQP4/APQ9 labeling. |
| 24828425 |
65054 |
Aqp9
|
Immunity |
The Western blotting data (Figure 7) verified the immunohistochemical findings (Figure 6), in that the protein expression levels of MMP2, AQP4, and AQP9 were all significantly higher in group M than in the other four groups (P<0.05). |
| 24828425 |
305081 |
Cd34
|
Immunity |
To evaluate the astrocytic and vascular localization of MMP2 and AQP4 in the ischemic penumbra of CIRI model rats, immunofluorescence analysis was employed using specific primary antibodies against MMP2, AQP4, GFAP (to label astrocytes), and CD34 (to label the endothelial cell components of blood vessels). |
| 24828425 |
287435 |
Cd68
|
Immunity |
Finally, immunohistochemical staining for MPO and CD68 was performed in the ischemic hemisphere, followed by quantification of MPO+ and CD8+ cells in the ischemic penumbra and the core (Figure 8C and 8F). |
| 24828425 |
24387 |
Gfap
|
Immunity |
To evaluate the astrocytic and vascular localization of MMP2 and AQP4 in the ischemic penumbra of CIRI model rats, immunofluorescence analysis was employed using specific primary antibodies against MMP2, AQP4, GFAP (to label astrocytes), and CD34 (to label the endothelial cell components of blood vessels). |
| 24828425 |
81686 |
Mmp2
|
Immunity |
To evaluate the astrocytic and vascular localization of MMP2 and AQP4 in the ischemic penumbra of CIRI model rats, immunofluorescence analysis was employed using specific primary antibodies against MMP2, AQP4, GFAP (to label astrocytes), and CD34 (to label the endothelial cell components of blood vessels). |
| 24828425 |
81686 |
Mmp2
|
Immunity |
Next, we explored the hypothesis that acupuncture and electroacupuncture can regulate MMP2, AQP4, and AQP9 expression in the ischemic penumbra and the core zone by performing an immunohistochemical analysis of target protein expression, followed by semi-quantitative measurement of the integrated optical density of MMP2/AQP4/APQ9 labeling. |
| 24828425 |
81686 |
Mmp2
|
Immunity |
The Western blotting data (Figure 7) verified the immunohistochemical findings (Figure 6), in that the protein expression levels of MMP2, AQP4, and AQP9 were all significantly higher in group M than in the other four groups (P<0.05). |
| 24828425 |
303413 |
Mpo
|
Immunity |
Finally, immunohistochemical staining for MPO and CD68 was performed in the ischemic hemisphere, followed by quantification of MPO+ and CD8+ cells in the ischemic penumbra and the core (Figure 8C and 8F). |
| 25074385 |
25712 |
Ifng
|
Immunity |
Spinal IFN-gamma and P2X4R expression levels were measured by immunohistochemistry, real-time PCR, enzyme immunoassay, and/or western blots. |
| 25120577 |
25491 |
Nes
|
Immunity |
Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin+, BrdU+ and nestin/BrdU+) were found in MCAO rats which had not been observed in normal group and sham-operated group. |
| 25120577 |
25491 |
Nes
|
Immunity |
By immunofluorescence double-staining, a large number of BrdU+, nestin+, and BrdU/nestin+ cells in MCAO groups were observed as shown in Figure 5, whereas no immune-positive cells were found in normal and sham-operated group. |
| 25120577 |
25491 |
Nes
|
Immunity |
Consistent with the results of immunofluorescence double-staining, model group shown a high expression of nestin mRNA compared to normal and sham-operated group especially in striatum followed by hippocampus and cortex suggesting there was different expression tendency in three cerebral regions after ischemia. |
| 25206406 |
24387 |
Gfap
|
Immunity |
After rats were subjected to electroacupuncture intervention at 15 and 30 Hz frequencies, glial fibrillary acidic protein immunoreactivity was observed in the cortex of the parietal lobe on the infarct side (coronal place at 3 mm posterior to bregma, sagittal plane at parietal cortex 4.5 mm lateral to the midline), with enlarged cell bodies and thickening fibers (Figure 1). |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Electroacupuncture and pinaverium bromide therapy both markedly decreased abdominal withdrawal reflex scores in rats with visceral hypersensitivity, and significantly decreased P2X4 receptor immunoreactivity in the colon and spinal cord. |
| 25206515 |
29659 |
P2rx4
|
Immunity |
These data suggest that electroacupuncture treatment can improve visceral hypersensitivity in rats with irritable bowel syndrome by diminishing P2X4 receptor immunoreactivity in the colon and spinal cord. |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Electroacupuncture at He-Mu points diminished P2X4 receptor immunoreactivity in the colon of rats with chronic visceral hypersensitivity |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Immunohistochemistry revealed that P2X4 receptor immunoreactivity was increased in the colon of rats with irritable bowel syndrome (P < 0.01). |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Compared with the model group, P2X4 receptor immunoreactivity was significantly lower in the electroacupuncture and pinaverium bromide groups (P < 0.01; Figure 2). |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Electroacupuncture at He-Mu points diminished P2X4 receptor immunoreactivity in spinal cord tissue of rats with chronic visceral hypersensitivity |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Immunohistochemistry revealed that P2X4 receptor immunoreactivity was increased in the spinal cord of rats with irritable bowel syndrome (P < 0.01). |
| 25206515 |
29659 |
P2rx4
|
Immunity |
Compared with the model group, P2X4 receptor immunoreactivity was significantly lower in the electroacupuncture and pinaverium bromide groups (P < 0.01; Figure 3). |
| 25206727 |
114115 |
P2rx2
|
Immunity |
Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. |
| 25206727 |
114115 |
P2rx2
|
Immunity |
Immunohistochemistry revealed that P2X2 receptor expression increased in rat dorsal root ganglia with irritable bowel syndrome (P < 0.01), suggesting P2X2 participated in visceral hypersensitivity. |
| 25206727 |
81739 |
P2rx3
|
Immunity |
Immunohistochemistry revealed that P2X3 receptor expression increased in dorsal root ganglia from rats with visceral hypersensitivity (P < 0.01), suggesting that P2X3 participated in visceral hypersensitivity. |
| 25206727 |
81739 |
P2rx3
|
Immunity |
Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. |
| 25206816 |
24225 |
Bdnf
|
Immunity |
Immunohistochemical staining exhibited that the expression of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the ventral tegmental area following acupuncture. |
| 25206816 |
24225 |
Bdnf
|
Immunity |
Immunohistochemical staining demonstrated that compared with the normal group, the number of brain-derived neurotrophic factor-positive cells and mean absorbance were greater in the ventral tegmental area of heroin relapse rats (P < 0.05). |
| 25206816 |
25453 |
Gdnf
|
Immunity |
Immunohistochemical staining exhibited that the expression of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the ventral tegmental area following acupuncture. |
| 25206816 |
25453 |
Gdnf
|
Immunity |
Immunohistochemical staining showed that compared with the normal group, the number of glial cell line-derived neurotrophic factor-positive cells and mean absorbance were greater in the ventral tegmental area of heroin relapse rats (P < 0.05). |
| 25292341 |
24494 |
Il1b
|
Immunity |
The levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in bronchoalveolar lavage fluid (BALF) and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. |
| 25292341 |
25723 |
Hcrt
|
Immunity |
The levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in bronchoalveolar lavage fluid (BALF) and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. |
| 25292341 |
24835 |
Tnf
|
Immunity |
The levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in bronchoalveolar lavage fluid (BALF) and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. |
| 25302705 |
498638 |
Msr1
|
Immunity |
Recent study showed that TNF-alpha secretion was enhanced in SR-A-/-mice, and could be responsible for the increased MMP activity and augmented risk of MI; SR-A has a role in the induction of innate immunity and plays a central role in cerebral ischemia/reperfusion injury. |
| 25302705 |
24835 |
Tnf
|
Immunity |
Recent study showed that TNF-alpha secretion was enhanced in SR-A-/-mice, and could be responsible for the increased MMP activity and augmented risk of MI; SR-A has a role in the induction of innate immunity and plays a central role in cerebral ischemia/reperfusion injury. |
| 25335789 |
59086 |
Tgfb1
|
Immunity |
In the EA group only, immunostaining showed strong expression of TGF-beta1 7 days after model preparation (p<0.05). |
| 25342636 |
84394 |
Dcx
|
Immunity |
Effects of EA on neuroblast differentiation at the onset of type-2 diabetes: In the ZLC group, DCX-immunoreactive neuroblasts were observed in the dentate gyrus. |
| 25342636 |
84394 |
Dcx
|
Immunity |
In the ZDF-Sham group, the number of DCX-immunoreactive neuroblast was significantly decreased as compared to that in the ZLC group (Fig. |
| 25342636 |
84394 |
Dcx
|
Immunity |
In addition, the dendrites of DCX-immunoreactive neuroblasts were poorly developed as compared to those in the ZLC group (Fig. |
| 25342636 |
84394 |
Dcx
|
Immunity |
In the ZDF-EA group, DCX-immunoreactive neuroblasts had well-developed dendrites, and the number of DCX-immunoreactive neuroblasts was significantly increased as compared to that in the ZDF-Sham group (Fig. |
| 25579380 |
24547 |
Mbp
|
Immunity |
We investigated whether treatment with BVA (0.25 and 0.8 mg/kg) at the Zusanli (ST36) acupoints, located lateral from the anterior border of the tibia, has a beneficial effect in a myelin basic protein (MBP)(68-82)-induced acute experimental autoimmune encephalomyelitis (EAE) rat model. |
| 25657670 |
25400 |
Camk2a
|
Immunity |
The integral grey value of CaMKII immunoreactivity was significantly higher in the OVX + CCI + electroacupuncture 2 weeks group compared with the CCI + electroacupuncture 2 weeks group (P < 0.05). |
| 25810743 |
54226 |
App
|
Immunity |
Data from both immunohistochemistry staining and Western blot showed that the level of synapsin-1 was markedly decreased owing to Abeta injection (P < 0.01). |
| 25810743 |
24949 |
Syn1
|
Immunity |
Data from both immunohistochemistry staining and Western blot showed that the level of synapsin-1 was markedly decreased owing to Abeta injection (P < 0.01). |
| 25878596 |
29577 |
Hes1
|
Immunity |
Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. |
| 25878596 |
29577 |
Hes1
|
Immunity |
Hes1 immunoreactivity in rat basal ganglia tissue |
| 25878596 |
29577 |
Hes1
|
Immunity |
Hes1-immunoreactive cells were mainly expressed in vascular endothelial and glial cells. |
| 25878596 |
29577 |
Hes1
|
Immunity |
Hes1 immunoreactivity was observed in the control group. |
| 25878596 |
29577 |
Hes1
|
Immunity |
At various time points, Hes1-immunoreactive cell number was significantly increased in the model group compared with the control group (P < 0.05). |
| 25878596 |
29577 |
Hes1
|
Immunity |
Moreover, Hes1-immunoreactive cell number was significantly lower in the acupuncture group than the model group at all time points (P < 0.05). |
| 25878596 |
29577 |
Hes1
|
Immunity |
Hes1 immunoreactivity was suppressed in the DAPT group, with significant differences between DAPT and model groups at 3, 7, and 14 days (P < 0.05). |
| 25878596 |
29577 |
Hes1
|
Immunity |
Hes1 immunoreactivity was inhibited in both the acupuncture and DAPT groups, but no significant difference was detected at any time point (P > 0.05; Table 3, Figure 3). |
| 25878596 |
25496 |
Notch1
|
Immunity |
Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch1 and Hes1 protein expression in rat basal ganglia. |
| 25878596 |
25496 |
Notch1
|
Immunity |
Notch1 immunoreactivity in rat basal ganglia tissue |
| 25878596 |
25496 |
Notch1
|
Immunity |
Notch1-immunoreactive cells were widely distributed in the cytoplasm and nucleus. |
| 25878596 |
25496 |
Notch1
|
Immunity |
Weak Notch1 immunoreactivity was observed in the control group. |
| 25878596 |
25496 |
Notch1
|
Immunity |
Notch1-immunoreactive cell number was significantly higher in the model group than in the control group at various time points (P < 0.05), and significantly lower in the DAPT group than the model group at 1, 3 (P < 0.01), and 7 (P < 0.05) days. |
| 25878596 |
25496 |
Notch1
|
Immunity |
Notch1-immunoreactive cell number was lower in the acupuncture group than in the model group at 1, 3, and 7 days (P < 0.05). |
| 26037401 |
24387 |
Gfap
|
Immunity |
Brain tissues were harvested for lactate concentration examination, immunohistochemical staining, Western blot and qRT-PCR analyses for the expressions of lactate transporter (monocarboxylate transporter 1, MCT1) and glial fibrillary acidic protein (GFAP). |
| 26037401 |
25027 |
Slc16a1
|
Immunity |
Brain tissues were harvested for lactate concentration examination, immunohistochemical staining, Western blot and qRT-PCR analyses for the expressions of lactate transporter (monocarboxylate transporter 1, MCT1) and glial fibrillary acidic protein (GFAP). |
| 26148746 |
24241 |
Calca
|
Immunity |
We used a similar procedure in the double immunohistochemical staining to examine the correlation between mast cells and SP or CGRP-positive nerve fibers, |
| 26148746 |
24241 |
Calca
|
Immunity |
The correlation of mast cells and SP- or CGRP-positive nerve fibers in the area of LI4 were examined by double immunohistochemical staining (Fig. 3, Fig. 4). |
| 26148746 |
24806 |
Tac1
|
Immunity |
We used a similar procedure in the double immunohistochemical staining to examine the correlation between mast cells and SP or CGRP-positive nerve fibers, |
| 26279808 |
25293 |
Aqp4
|
Immunity |
Aquaporin-4 expression in brain tissue was studied through immunohistochemical and western-blot analysis. |
| 26339273 |
310553 |
Tlr2
|
Immunity |
TLR2 protein expression was determined using immunohistochemistry and quantified by the total area of staining and the integrated optical density. |
| 26339273 |
310553 |
Tlr2
|
Immunity |
It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa. |
| 26345700 |
84577 |
Hdac2
|
Immunity |
HDAC2 immunostaining revealed expression in the COPD group was significantly lower than in the Control group, while acupuncture treatment significantly enhanced HDAC2 protein expression (figure 3A, B). |
| 26386034 |
314322 |
Fos
|
Immunity |
Concentrations of serotonin (5-hydroxytryptamine, 5-HT) in the duodenum and c-Fos expression in the nucleus of the solitary tract (NTS) were measured using high performance liquid chromatography and immunohistochemistry, respectively. |
| 26386034 |
314322 |
Fos
|
Immunity |
Cisplatin (6 mg/kg ip) produced greater c-Fos-like immunoreactivity than saline in the NTS (cisplatin+sham vs saline+sham: 67+-6 vs 4+-2, p<0.01; figure 4C). |
| 26438555 |
314322 |
Fos
|
Immunity |
HomeCageScan was used to measure effects on behaviour, and immunofluorescence staining was used to examine neural activation (c-Fos immunoreactivity) in the PAG, RMg, and TNC. |
| 26438555 |
314322 |
Fos
|
Immunity |
There were no significant differences between the SA and Model groups in behaviour or c-Fos immunoreactivity. |
| 26438555 |
314322 |
Fos
|
Immunity |
c-Fos immunofluorescence analysis |
| 26438555 |
314322 |
Fos
|
Immunity |
Representative images and the density of c-Fos-immunoreactive cells in the PAG, RMg, and TNC are shown in figures 2-4, respectively. |
| 26770252 |
50689 |
Mapk3
|
Immunity |
The pain withdrawal thresholds (PWTs) and anxiety behavior were measured, and p-ERK protein expression and immunoreactivity cells in ACC were detected. |
| 26770252 |
116590 |
Mapk1
|
Immunity |
The pain withdrawal thresholds (PWTs) and anxiety behavior were measured, and p-ERK protein expression and immunoreactivity cells in ACC were detected. |
| 26770252 |
50689 |
Mapk3
|
Immunity |
In the control group, p-ERK was expressed largely in laminae II-III (Figure 3(a)), whereas SNL resulted in a wide distribution of p-ERK-immunoreactive cells throughout laminae II-VI (Figure 3(b)). |
| 26770252 |
116590 |
Mapk1
|
Immunity |
In the control group, p-ERK was expressed largely in laminae II-III (Figure 3(a)), whereas SNL resulted in a wide distribution of p-ERK-immunoreactive cells throughout laminae II-VI (Figure 3(b)). |
| 26770252 |
50689 |
Mapk3
|
Immunity |
In comparison with SNL rats, animals that received EA stimulation showed no difference in p-ERK-immunoreactive cell numbers (Figure 3(c)). |
| 26770252 |
116590 |
Mapk1
|
Immunity |
In comparison with SNL rats, animals that received EA stimulation showed no difference in p-ERK-immunoreactive cell numbers (Figure 3(c)). |
| 26770252 |
50689 |
Mapk3
|
Immunity |
Very few p-ERK-immunoreactive neurons were found in the ACC in non-SNL control rats (Figure 4(a)). |
| 26770252 |
116590 |
Mapk1
|
Immunity |
Very few p-ERK-immunoreactive neurons were found in the ACC in non-SNL control rats (Figure 4(a)). |
| 26770252 |
50689 |
Mapk3
|
Immunity |
Our immunofluorescence assay revealed an overexpression of p-ERK1/2-positive cells in the ACC of rats on day 12 after surgery, and western blots showed a higher level of p-ERK1/2 protein expression in the SNL group than in non-SNL controls (Figures 3(a), 3(b), and 3(f)-3(h), P < 0.01). |
| 26770252 |
116590 |
Mapk1
|
Immunity |
Interestingly, p-ERK1/2-immunoreactive cell number and protein expression were significantly lower in rats that underwent mMA and sMA stimulation than in SNL rats (Figures 3(b) and 3(d)-3(h), P < 0.01). |
| 26770252 |
116590 |
Mapk1
|
Immunity |
There was no significant difference in p-ERK1/2-immunoreactive cells or protein expression between the EA and SNL groups (Figures 3(b), 3(c), 3(f), and 3(g), P > 0.05). |
| 26895770 |
54226 |
App
|
Immunity |
Reduced Abeta levels, and co-localisation of Abeta and LC3II, were observed following EA treatment by immunofluorescence staining. |
| 26895770 |
54226 |
App
|
Immunity |
Reduced Abeta levels, and co-localisation of Abeta and LC3II, were observed following EA treatment by immunofluorescence staining. |
| 26895770 |
362245 |
Map1lc3a
|
Immunity |
Reduced Abeta levels, and co-localisation of Abeta and LC3II, were observed following EA treatment by immunofluorescence staining. |
| 26896072 |
314322 |
Fos
|
Immunity |
Expression of c-fos was quantified using immunohistochemistry. |
| 27085942 |
24185 |
Akt1
|
Immunity |
The proportions of cells with positive Bcl-2 and Bax expression were determined by immunohistochemical assays, whilst the expression profiles of p-AKT and p-ERK in spinal cord tissues were evaluated by western blotting. |
| 27085942 |
24887 |
Bax
|
Immunity |
The proportions of cells with positive Bcl-2 and Bax expression were determined by immunohistochemical assays, whilst the expression profiles of p-AKT and p-ERK in spinal cord tissues were evaluated by western blotting. |
| 27085942 |
24224 |
Bcl2
|
Immunity |
The proportions of cells with positive Bcl-2 and Bax expression were determined by immunohistochemical assays, whilst the expression profiles of p-AKT and p-ERK in spinal cord tissues were evaluated by western blotting. |
| 27085942 |
50689 |
Mapk3
|
Immunity |
The proportions of cells with positive Bcl-2 and Bax expression were determined by immunohistochemical assays, whilst the expression profiles of p-AKT and p-ERK in spinal cord tissues were evaluated by western blotting. |
| 27085942 |
116590 |
Mapk1
|
Immunity |
The proportions of cells with positive Bcl-2 and Bax expression were determined by immunohistochemical assays, whilst the expression profiles of p-AKT and p-ERK in spinal cord tissues were evaluated by western blotting. |
| 27085942 |
24494 |
Il1b
|
Immunity |
Levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6, IL-1beta, and nuclear factor kappa-beta, were determined by enzyme-linked immunosorbent assay. |
| 27085942 |
24498 |
Il6
|
Immunity |
Levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6, IL-1beta, and nuclear factor kappa-beta, were determined by enzyme-linked immunosorbent assay. |
| 27085942 |
24835 |
Tnf
|
Immunity |
Levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6, IL-1beta, and nuclear factor kappa-beta, were determined by enzyme-linked immunosorbent assay. |
| 27127487 |
170945 |
Chrna2
|
Immunity |
Warming yang and invigorating qi acupuncture alters acetylcholine receptor expression in the neuromuscular junction of rats with experimental autoimmune myasthenia gravis |
| 27127487 |
170945 |
Chrna2
|
Immunity |
We found that area and the integrated optical density of the immunoreactivity for the acetylcholine receptor at the neuromuscular junction of the phrenic nerve was significantly increased following acupuncture treatment. |
| 27127487 |
170945 |
Chrna2
|
Immunity |
These findings suggest that warming yang and invigorating qi acupuncture treatment increases acetylcholine receptor expression at the neuromuscular junction in a rat model of autoimmune myasthenia gravis. |
| 27127487 |
170945 |
Chrna2
|
Immunity |
Compared with the control group, the averages of the immunofluorescence-positive area and the integrated optical density of the nicotinic AChR antibody immunoreactivity at neuromuscular junction in the phrenic nerve were lower in all three groups of rats with EAMG (P < 0.01). |
| 27127487 |
170945 |
Chrna2
|
Immunity |
Here, we used confocal laser scanning microscopy to examine the area and density of immunoreactivity for an antibody to the nicotinic acetylcholine receptor at the neuromuscular junction in the phrenic nerve of rats with experimental autoimmune myasthenia gravis following warming yang and invigorating qi acupuncture therapy. |
| 27127487 |
59086 |
Tgfb1
|
Immunity |
Warming yang and invigorating qi acupuncture treatment has been shown to reduce serum inflammatory cytokine expression and increase transforming growth factor beta expression in rats with experimental autoimmune myasthenia gravis. |
| 27179524 |
298199 |
Plin2
|
Immunity |
Consistent with the previously published data, perinatal nicotine exposure resulted in decreased PPARgamma signaling, as indicated by decreased PPARgamma (Western analysis and immunostaining) and its downstream target adipocyte differentiation-related protein (ADRP) (Western analysis) levels, and blockage of these changes by the concomitant EA treatment (Fig. |
| 27179524 |
84353 |
Ctnnb1
|
Immunity |
Furthermore, perinatal nicotine exposure-induced up-regulation of beta-catenin (Western analysis, immunostaining) and LEF-1 (Western analysis), two key mediators of Wnt signaling, was also blocked in the EA-treated group (Fig. |
| 27179524 |
161452 |
Lef1
|
Immunity |
Furthermore, perinatal nicotine exposure-induced up-regulation of beta-catenin (Western analysis, immunostaining) and LEF-1 (Western analysis), two key mediators of Wnt signaling, was also blocked in the EA-treated group (Fig. |
| 27179524 |
25664 |
Pparg
|
Immunity |
Consistent with the previously published data, perinatal nicotine exposure resulted in decreased PPARgamma signaling, as indicated by decreased PPARgamma (Western analysis and immunostaining) and its downstream target adipocyte differentiation-related protein (ADRP) (Western analysis) levels, and blockage of these changes by the concomitant EA treatment (Fig. |
| 27179524 |
303181 |
Wnt3a
|
Immunity |
Furthermore, perinatal nicotine exposure-induced up-regulation of beta-catenin (Western analysis, immunostaining) and LEF-1 (Western analysis), two key mediators of Wnt signaling, was also blocked in the EA-treated group (Fig. |
| 27301303 |
24473 |
Htr1a
|
Immunity |
Mast cells are known as immune cells that play an active role in the immune response through degranulation to release biological substances including tryptase, histamine, and serotonin (5-HT) et al. |
| 27301303 |
24473 |
Htr1a
|
Immunity |
So far, 5-HT levels in the central nervous and in the blood of platelets have been assayed by extraction, followed by high precision liquid chromatography (HPLC) analysis14 and enzyme-linked immunosorbent assay (ELISA) |
| 27401747 |
54226 |
App
|
Immunity |
The rats' behaviour was examined using the Morris water maze test, and protein expression of Abeta, hyperphosphorylated tau protein (p-Tau), PPAR-gamma, and hyperphosphorylated p38 mitogen activated protein kinase (p38MAPK) in the hippocampal CA1 region was examined by immunohistochemistry and Western blotting. |
| 27401747 |
81649 |
Mapk14
|
Immunity |
The rats' behaviour was examined using the Morris water maze test, and protein expression of Abeta, hyperphosphorylated tau protein (p-Tau), PPAR-gamma, and hyperphosphorylated p38 mitogen activated protein kinase (p38MAPK) in the hippocampal CA1 region was examined by immunohistochemistry and Western blotting. |
| 27401747 |
25664 |
Pparg
|
Immunity |
The rats' behaviour was examined using the Morris water maze test, and protein expression of Abeta, hyperphosphorylated tau protein (p-Tau), PPAR-gamma, and hyperphosphorylated p38 mitogen activated protein kinase (p38MAPK) in the hippocampal CA1 region was examined by immunohistochemistry and Western blotting. |
| 27594888 |
116562 |
Il2
|
Immunity |
In this study, in order to investigate the immune function, serum IgG, IgM, IL-2, and IL-6 levels were measured. |
| 27594888 |
116562 |
Il2
|
Immunity |
IL-2 is a very important cytokine for bidirectional immune regulation. |
| 27594888 |
24498 |
Il6
|
Immunity |
IL-6 is a proinflammatory cytokine and correlates with immunoreaction closely [30, 31]. |
| 27594888 |
299357 |
Igh-6
|
Immunity |
The serum immunoglobulin G (IgG) and immunoglobulin M (IgM) levels were measured using commercially available sandwich ELISA kits (Xiang Sheng Biotechnology, Shanghai, China) according to the manufacturer's instructions. |
| 27594888 |
299357 |
Igh-6
|
Immunity |
The serum immunoglobulin G (IgG) and immunoglobulin M (IgM) levels were measured using commercially available sandwich ELISA kits (Xiang Sheng Biotechnology, Shanghai, China) according to the manufacturer's instructions. |
| 27594888 |
299357 |
Igh-6
|
Immunity |
In this study, in order to investigate the immune function, serum IgG, IgM, IL-2, and IL-6 levels were measured. |
| 27594888 |
299357 |
Igh-6
|
Immunity |
IgG and IgM are the main components of humoral immunity, and serum levels of IgG and IgM are commonly low in immunocompromised patients |
| 27852302 |
25402 |
Casp3
|
Immunity |
Cell infarction volume (TTC stain), neuronal apoptosis (markers: TUNEL and Caspase-3), activation of microglia (marker: Iba1) and astrocytes (marker: GFAP), and tumor necrosis factor (TNF)-alpha expression in the microglia and astrocytes were evaluated by immunofluorescence. |
| 27852302 |
24387 |
Gfap
|
Immunity |
Cell infarction volume (TTC stain), neuronal apoptosis (markers: TUNEL and Caspase-3), activation of microglia (marker: Iba1) and astrocytes (marker: GFAP), and tumor necrosis factor (TNF)-alpha expression in the microglia and astrocytes were evaluated by immunofluorescence. |
| 27852302 |
29427 |
Aif1
|
Immunity |
Cell infarction volume (TTC stain), neuronal apoptosis (markers: TUNEL and Caspase-3), activation of microglia (marker: Iba1) and astrocytes (marker: GFAP), and tumor necrosis factor (TNF)-alpha expression in the microglia and astrocytes were evaluated by immunofluorescence. |
| 27852302 |
24835 |
Tnf
|
Immunity |
Cell infarction volume (TTC stain), neuronal apoptosis (markers: TUNEL and Caspase-3), activation of microglia (marker: Iba1) and astrocytes (marker: GFAP), and tumor necrosis factor (TNF)-alpha expression in the microglia and astrocytes were evaluated by immunofluorescence. |
| 28051128 |
314322 |
Fos
|
Immunity |
To obtain more insights into the central mechanism by which EA regulates POI, immunofluorescence analysis of the brain for c-fos was performed. |
| 28478802 |
287847 |
Rbfox3
|
Immunity |
Meanwhile, the NeuN expression was examined in the hippocampus, the expression levels of Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase2(NOX2) was detected by immunofluorescence staining and western blot. |
| 28478802 |
66021 |
Cybb
|
Immunity |
Meanwhile, the NeuN expression was examined in the hippocampus, the expression levels of Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase2(NOX2) was detected by immunofluorescence staining and western blot. |
| 28493083 |
25402 |
Casp3
|
Immunity |
A549 cells were treated by rat serum from the CPB and CPB + EAc groups, and cleaved caspase-3 activity was detected by fluorescent immunohistochemistry. |
| 28507480 |
24241 |
Calca
|
Immunity |
After 7 days, the mRNA and protein expression of Nestin, neuron specific nuclear protein (NeuN) and calcitonin gene related peptide (CGRP) were detected by real time RT-PCR, Western blot and immunohistochemistry respectively. |
| 28507480 |
24241 |
Calca
|
Immunity |
The immunohistochemical results also showed that Nestin protein level was raised to some degree, but NeuN and CGRP protein levels were markedly declined in SCI group (P < 0.01 or P < 0.05), which were all raised in SCI+EA group (P < 0.05 or 0.01) (Figure 1E-H(Fig. |
| 28507480 |
24241 |
Calca
|
Immunity |
Moreover, the results of real time PCR, Western blot and immunohistochemical staining were also shown that the expression of Nestin, NeuN and CGRP mRNA and protein in miR group was significantly reduced compared with EA group (P < 0.05 or 0.01) (Figure 3B-H(Fig. |
| 28507480 |
25491 |
Nes
|
Immunity |
After 7 days, the mRNA and protein expression of Nestin, neuron specific nuclear protein (NeuN) and calcitonin gene related peptide (CGRP) were detected by real time RT-PCR, Western blot and immunohistochemistry respectively. |
| 28507480 |
25491 |
Nes
|
Immunity |
The immunohistochemical results also showed that Nestin protein level was raised to some degree, but NeuN and CGRP protein levels were markedly declined in SCI group (P < 0.01 or P < 0.05), which were all raised in SCI+EA group (P < 0.05 or 0.01) (Figure 1E-H(Fig. |
| 28507480 |
25491 |
Nes
|
Immunity |
Moreover, the results of real time PCR, Western blot and immunohistochemical staining were also shown that the expression of Nestin, NeuN and CGRP mRNA and protein in miR group was significantly reduced compared with EA group (P < 0.05 or 0.01) (Figure 3B-H(Fig. |
| 28507480 |
287847 |
Rbfox3
|
Immunity |
After 7 days, the mRNA and protein expression of Nestin, neuron specific nuclear protein (NeuN) and calcitonin gene related peptide (CGRP) were detected by real time RT-PCR, Western blot and immunohistochemistry respectively. |
| 28507480 |
287847 |
Rbfox3
|
Immunity |
The immunohistochemical results also showed that Nestin protein level was raised to some degree, but NeuN and CGRP protein levels were markedly declined in SCI group (P < 0.01 or P < 0.05), which were all raised in SCI+EA group (P < 0.05 or 0.01) (Figure 1E-H(Fig. |
| 28507480 |
287847 |
Rbfox3
|
Immunity |
Moreover, the results of real time PCR, Western blot and immunohistochemical staining were also shown that the expression of Nestin, NeuN and CGRP mRNA and protein in miR group was significantly reduced compared with EA group (P < 0.05 or 0.01) (Figure 3B-H(Fig. |
| 28546432 |
29467 |
Ddit3
|
Immunity |
PERK, eIF2a, CHOP, IRE1 and JNK gene expression and protein expression were measured using quantitative real-time PCR (RT-qPCR) assay and immunohistochemical assay, respectively. |
| 28546432 |
502531 |
Eif2a
|
Immunity |
PERK, eIF2a, CHOP, IRE1 and JNK gene expression and protein expression were measured using quantitative real-time PCR (RT-qPCR) assay and immunohistochemical assay, respectively. |
| 28546432 |
116554 |
Mapk8
|
Immunity |
PERK, eIF2a, CHOP, IRE1 and JNK gene expression and protein expression were measured using quantitative real-time PCR (RT-qPCR) assay and immunohistochemical assay, respectively. |
| 28600329 |
24241 |
Calca
|
Immunity |
30 min later, the rats were euthanased and cervical (C3-6) DRGs removed for measurement of immunoreactivity and mRNA expression of SP/CGRP and the GABAergic neuronal marker glutamic acid decarboxylase 67 (GAD67). |
| 28600329 |
24241 |
Calca
|
Immunity |
Effects of EA on SP and CGRP immunoreactivity |
| 28600329 |
24241 |
Calca
|
Immunity |
Immunofluorescence staining showed that SP- and CGRP-IR positive substances were granular in appearance and distributed throughout the cytoplasm. |
| 28600329 |
24379 |
Gad1
|
Immunity |
30 min later, the rats were euthanased and cervical (C3-6) DRGs removed for measurement of immunoreactivity and mRNA expression of SP/CGRP and the GABAergic neuronal marker glutamic acid decarboxylase 67 (GAD67). |
| 28600329 |
24379 |
Gad1
|
Immunity |
Effects of EA on GAD67 immunoreactivity |
| 28913350 |
24387 |
Gfap
|
Immunity |
Effect of EA on Ki67, GFAP, and Nestin Immunoreactive Cells in Ischemia-Reperfusion-Injured Rats |
| 28913350 |
24387 |
Gfap
|
Immunity |
In the penumbra area, the number of GFAP immunoreactive cells in the control, sham, EA1, and EA2 groups was higher than that in the normal group (all p < 0.001; Table 2, Figure 3). |
| 28913350 |
24387 |
Gfap
|
Immunity |
The number of GFAP immunoreactive cells in the EA1 group was higher than that in the control, sham, and EA2 groups (all p < 0.05; Table 2, Figure 3); moreover, the number of GFAP immunoreactive cells was similar between the two groups among the control, sham, and EA2 groups (all p > 0.05; Table 2, Figure 3). |
| 28913350 |
24387 |
Gfap
|
Immunity |
In addition, 2 Hz reduced nestin immunoreactive cells in the penumbra area and the ischemic core area; 2 Hz EA also reduced Ki67 immunoreactive cells and increased glial fibrillary acidic protein immunoreactive cells in the penumbra area. |
| 28913350 |
25491 |
Nes
|
Immunity |
In addition, 2 Hz reduced nestin immunoreactive cells in the penumbra area and the ischemic core area; 2 Hz EA also reduced Ki67 immunoreactive cells and increased glial fibrillary acidic protein immunoreactive cells in the penumbra area. |
| 28913350 |
25491 |
Nes
|
Immunity |
Effect of EA on Ki67, GFAP, and Nestin Immunoreactive Cells in Ischemia-Reperfusion-Injured Rats |
| 28913350 |
25491 |
Nes
|
Immunity |
In the penumbra area, no prominent nestin immunoreactive cells were noted in the normal group (Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
Additionally, the number of nestin immunoreactive cells in the control and sham groups was higher than that in the EA1 and EA2 groups (all p < 0.05; Table 2, Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
The number of nestin immunoreactive cells was also higher in the EA2 group than that in the EA1 group (p < 0.05; Table 2, Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
Finally, the number of nestin immunoreactive cells was similar between the control and sham groups (p > 0.05; Table 2, Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
In the ischemic core area, no prominent nestin immunoreactive cells were noted in the normal group (Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
The number of nestin immunoreactive cells in the control and sham groups was also higher than that in the EA1 and EA2 groups (all p < 0.05; Table 2, Figure 4). |
| 28913350 |
25491 |
Nes
|
Immunity |
Furthermore, the number of nestin immunoreactive cells was higher in the EA2 group than in the EA1 group (p < 0.05; Table 2, Figure 4), and the number of such cells was similar between the control and sham groups (p > 0.05; Table 2, Figure 4). |
| 29123119 |
24241 |
Calca
|
Immunity |
To determine whether activation of CGRP and vasodilation occurred at neurogenic spots, CGRP immunohistochemistry was performed for the skin over the wrist in the hypertensive rats. |
| 29231528 |
25459 |
Hmgb1
|
Immunity |
CONCLUSIONS: (1) EA at digestive system-related lower he-sea points, through IL-1beta, HMGB 1 and nAchR alpha7, could regulate immune response, lighten inflammatory reaction and reduce mucosal injury, which could realize the intervention effect on AGML rats. |
| 29231528 |
24494 |
Il1b
|
Immunity |
CONCLUSIONS: (1) EA at digestive system-related lower he-sea points, through IL-1beta, HMGB 1 and nAchR alpha7, could regulate immune response, lighten inflammatory reaction and reduce mucosal injury, which could realize the intervention effect on AGML rats. |
| 29231528 |
25302 |
Chrna7
|
Immunity |
CONCLUSIONS: (1) EA at digestive system-related lower he-sea points, through IL-1beta, HMGB 1 and nAchR alpha7, could regulate immune response, lighten inflammatory reaction and reduce mucosal injury, which could realize the intervention effect on AGML rats. |
| 27707699 |
25601 |
Oprm1
|
Immunity |
Expression of mu opioid receptors (MORs) in the L3-L5 dorsal root ganglion (DRG) was determined by immunofluorescence staining and Western blotting after treatment. |
| 27707699 |
25601 |
Oprm1
|
Immunity |
Expression of mu opioid receptors (MORs) in the L3-L5 dorsal root ganglion (DRG) was determined by immunofluorescence staining and Western blotting after treatment. |
| 27707699 |
25601 |
Oprm1
|
Immunity |
Immunofluorescence staining for MOR expression on POD 15 after CCI demonstrated that MORs appeared to be located predominantly in the medium and small sized DRG neurons (figure 5). |
| 27843474 |
24473 |
Htr1a
|
Immunity |
Radioimmunoassay and high-performance liquid chromatography were used to evaluate the expression of 5-hydroxytryptamine (HT) in the plasma and three-key structure of the descending pain modulatory system. |
| 28672900 |
24473 |
Htr1a
|
Immunity |
It decreased 5-hydroxytryptamine (5-HT) levels and immunoreactive expressions in the dorsal raphe nucleus (DRN). |
| 28672900 |
24473 |
Htr1a
|
Immunity |
It decreased 5-hydroxytryptamine (5-HT) levels and immunoreactive expressions in the dorsal raphe nucleus (DRN). |
| 28672900 |
24473 |
Htr1a
|
Immunity |
Distribution of 5-HT-immunoreactive cells in the DRN of rats was investigated at bregma -7.5, -8.0 and -8.5 mm (Fig. 6A-C, respectively). |
| 28672900 |
24473 |
Htr1a
|
Immunity |
The abundance of 5-HT-immunoreactive cells in the DRN was higher at bregma -8.00 mm than at bregma -7.50 and -8.50 mm. |
| 37179843 |
25305 |
Cort
|
Immunity |
Enzyme-linked immunosorbent assay was applied to check the expressions of the hypothalamic-pituitary-adrenal axis, including CORT, CRH, and ACTH in serum. |
| 37179843 |
81648 |
Crh
|
Immunity |
Enzyme-linked immunosorbent assay was applied to check the expressions of the hypothalamic-pituitary-adrenal axis, including CORT, CRH, and ACTH in serum. |
| 30089868 |
56718 |
Mtor
|
Immunity |
Effects of acupuncture at HT8 on the expressions of p-mTOR in the CA1, CA3 and dentate gyrus (DG) of the hippocampus using immunofluorescent analysis |
| 34636496 |
29197 |
Il18
|
Immunity |
The mRNA and protein expressions of interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) in the PFC were measured by the real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. |
| 34636496 |
24494 |
Il1b
|
Immunity |
The mRNA and protein expressions of interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) in the PFC were measured by the real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. |
| 19429024 |
24604 |
Npy
|
Immunity |
We investigated the influence of acupuncture therapy on chronic CORT-induced behavioral responses to the forced swimming test (FST) and elevated plus maze (EPM) and expression of neuropeptide Y (NPY) in the rat brain using immunohistochemistry. |
| 29636708 |
24225 |
Bdnf
|
Immunity |
The expression of serum BDNF was detected by enzyme-linked immunosorbent assay (ELISA), the hippocampal BDNF, acetylation levels in histone H3 lysine 9 (acH3K9), and HDAC2 by Western blot, the hippocampal mRNA of BDNF by RT-polymerase chain reaction (PCR). |
| 29636708 |
84577 |
Hdac2
|
Immunity |
The expression of serum BDNF was detected by enzyme-linked immunosorbent assay (ELISA), the hippocampal BDNF, acetylation levels in histone H3 lysine 9 (acH3K9), and HDAC2 by Western blot, the hippocampal mRNA of BDNF by RT-polymerase chain reaction (PCR). |
| 31785199 |
24599 |
Nos2
|
Immunity |
Enzyme-linked immunoassay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and the N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A, and NR2B in the rat plasma and hippocampus. |
| 31785199 |
24598 |
Nos1
|
Immunity |
Enzyme-linked immunoassay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and the N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A, and NR2B in the rat plasma and hippocampus. |
| 31785199 |
24409 |
Grin2a
|
Immunity |
Enzyme-linked immunoassay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and the N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A, and NR2B in the rat plasma and hippocampus. |
| 31785199 |
24410 |
Grin2b
|
Immunity |
Enzyme-linked immunoassay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and the N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A, and NR2B in the rat plasma and hippocampus. |
| 31785199 |
24408 |
Grin1
|
Immunity |
Enzyme-linked immunoassay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and the N-methyl-D-aspartate (NMDA) receptor subunits, NR1, NR2A, and NR2B in the rat plasma and hippocampus. |
| 31217804 |
314322 |
Fos
|
Immunity |
To investigate the effects of EA on hyperalgesia, we performed immunofluorescence analyses and western blotting to examine the number of c-Fos-positive cells and levels of c-Fos protein in the TG (Figure 3). |
| 31217804 |
24241 |
Calca
|
Immunity |
To determine whether EA influences the expression of vasoactive neurotransmitters in the dura mater, we performed immunofluorescence analyses of CGRP and VIP expression. |
| 31217804 |
117064 |
Vip
|
Immunity |
To determine whether EA influences the expression of vasoactive neurotransmitters in the dura mater, we performed immunofluorescence analyses of CGRP and VIP expression. |
| 36510338 |
282817 |
Pycard
|
Immunity |
We can still visualize that in the Immunohistochemistry results (Figure 5), the NLRP3 inflammasome and related proteins (ASC, Caspase-1, IL-1beta, and IL-18) in the KOA group showed significantly higher expression of protein-positive cells and heavier staining in the same field of view compared to the control group. |
| 36510338 |
25166 |
Casp1
|
Immunity |
We can still visualize that in the Immunohistochemistry results (Figure 5), the NLRP3 inflammasome and related proteins (ASC, Caspase-1, IL-1beta, and IL-18) in the KOA group showed significantly higher expression of protein-positive cells and heavier staining in the same field of view compared to the control group. |
| 36510338 |
29197 |
Il18
|
Immunity |
We can still visualize that in the Immunohistochemistry results (Figure 5), the NLRP3 inflammasome and related proteins (ASC, Caspase-1, IL-1beta, and IL-18) in the KOA group showed significantly higher expression of protein-positive cells and heavier staining in the same field of view compared to the control group. |
| 36510338 |
24494 |
Il1b
|
Immunity |
We can still visualize that in the Immunohistochemistry results (Figure 5), the NLRP3 inflammasome and related proteins (ASC, Caspase-1, IL-1beta, and IL-18) in the KOA group showed significantly higher expression of protein-positive cells and heavier staining in the same field of view compared to the control group. |
| 36510338 |
287362 |
Nlrp3
|
Immunity |
In this study, we aim to investigate the potential therapeutic effect of EA on the rat model of KOA induced by monosodium iodoacetate (MIA) and its relationship with NLRP3 inflammasome by immunohistochemistry and western blot. |
| 36510338 |
287362 |
Nlrp3
|
Immunity |
Immunohistochemistry and western blot showed significant inhibition of NLRP3 inflammasome-associated protein. |
| 36510338 |
287362 |
Nlrp3
|
Immunity |
We can still visualize that in the Immunohistochemistry results (Figure 5), the NLRP3 inflammasome and related proteins (ASC, Caspase-1, IL-1beta, and IL-18) in the KOA group showed significantly higher expression of protein-positive cells and heavier staining in the same field of view compared to the control group. |
| 38038835 |
24806 |
Tac1
|
Immunity |
Immunohistochemical staining was performed to detect 5-HT and substance P (SP) expressions. |
| 38038835 |
79246 |
Htr3a
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
79246 |
Htr3a
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
282821 |
Has1
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
25694 |
Has2
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
266805 |
Has3
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
24473 |
Htr1a
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
25324 |
Htr4
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
25324 |
Htr4
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
25553 |
Slc6a4
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 38038835 |
24848 |
Tph1
|
Immunity |
The levels of hyaluronic acid (HA), bradykinin (BK), prostaglandin I2 (PGI2) in skin tissue, 5-HT, tryptophan hydroxylase 1 (TPH1), serotonin transporters (SERT), 5-HT 3 receptor (5-HT3R), and 5-HT 4 receptor (5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay (ELISA). |
| 31775332 |
24387 |
Gfap
|
Immunity |
We examined the changes of immunostaining intensity of astrocytic marker glial fibrillary acidic protein (GFAP) and microglial marker OX42 in SCDH. |
| 31775332 |
24387 |
Gfap
|
Immunity |
Immunofluorescence showed that paclitaxel treatment significantly increased the staining intensity of GFAP and the number of GFAP positive cells in SCDH (Figure 8A-C). |
| 31775332 |
287847 |
Rbfox3
|
Immunity |
Our immunofluorescence study revealed that the percentage of TRPV1 immune positive (TRPV1+) DRG neurons among all DRG neurons (stained with NeuN) and TRPV1 immunofluorescent staining intensity were both significantly increased in the paclitaxel-treated group (Figure 2A-C). |
| 31775332 |
83810 |
Trpv1
|
Immunity |
Our immunofluorescence study revealed that the percentage of TRPV1 immune positive (TRPV1+) DRG neurons among all DRG neurons (stained with NeuN) and TRPV1 immunofluorescent staining intensity were both significantly increased in the paclitaxel-treated group (Figure 2A-C). |
| 33398365 |
29659 |
P2rx4
|
Immunity |
The expression levels of spinal P2X4R were determined using reverse transcription-quantitative PCR, western blotting analysis and immunofluorescence staining. |
| 30431067 |
54226 |
App
|
Immunity |
As illustrated by immunochemical detection, following the establishment of the cognitive dysfunction model, the production and distribution of TOMM40, TIMM17A and Abeta were all increased in the rat brain tissues (Fig. |
| 30431067 |
54311 |
Timm17a
|
Immunity |
As illustrated by immunochemical detection, following the establishment of the cognitive dysfunction model, the production and distribution of TOMM40, TIMM17A and Abeta were all increased in the rat brain tissues (Fig. |
| 30431067 |
308416 |
Tomm40
|
Immunity |
As illustrated by immunochemical detection, following the establishment of the cognitive dysfunction model, the production and distribution of TOMM40, TIMM17A and Abeta were all increased in the rat brain tissues (Fig. |
| 33229729 |
84350 |
Dnmt1
|
Immunity |
Compared with the model group, the expression of DNMT1 in the inhibitor group was decreased (P < 0.05 in immunofluorescence staining, P < 0.05 in western blot assay), but increased in the PEA + inhibitor (P < 0.01 in immunofluorescence staining, P < 0.05 in western blot assay), PMA (P < 0.01 in immunofluorescence staining, P < 0.05 in western blot) and PEA groups (P < 0.01 in immunofluorescence staining, P < 0.05 in western blot). |
| 33229729 |
84350 |
Dnmt1
|
Immunity |
In addition, the expression levels of DNMT1 were decreased in the PEA + inhibitor (P < 0.01 in immunofluorescence staining, P < 0.01 in western blot assay), inhibitor (P < 0.01 in immunofluorescence staining, P < 0.01 in western blot) and PMA groups (P < 0.05 in immunofluorescence staining, P < 0.05 in western blot assay) compared with the PEA group. |
| 36816326 |
24932 |
Cd4
|
Immunity |
As such, these results indicate that VD is associated with an increase in the numbers of CD4+RORgammat+ and RORgammat+ in the brains of these VD rats, consistent with a dominant pro-inflammatory Th17 immune response, whereas acupuncture treatment was sufficient to reverse these phenotypes and to inhibiting inflammatory activity, thereby reducing the severity of VD-related neuroinflammation (Fig. |
| 34804173 |
78971 |
Birc3
|
Immunity |
Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and neuronal nuclear protein (NeuN) double staining and cellular inhibitor of apoptosis protein-1 (cIAP1) and NeuN immunofluorescence double staining were used to detect the short-term and long-term neuroprotective effects of scalp acupuncture combined with treadmill training on pMCAO rats. |
| 34804173 |
78971 |
Birc3
|
Immunity |
The double immunofluorescence staining results showed that multitudinous cIAP1 and NeuN positive neurons existed in the cortex penumbra of the ischemic brain in the sham-operated group (Figure 4). |
| 34804173 |
78971 |
Birc3
|
Immunity |
To further investigate the influence of SA-T on the expression patterns of cIAP1 in the cIAP1 shRNA interference pMCAO model, immunohistochemistry and Western blotting were used to compare cIAP1 protein levels in the five groups on the 3rd, 7th, and 14th days (Figures 7(c) and 7(d)). |
| 34804173 |
287847 |
Rbfox3
|
Immunity |
Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and neuronal nuclear protein (NeuN) double staining and cellular inhibitor of apoptosis protein-1 (cIAP1) and NeuN immunofluorescence double staining were used to detect the short-term and long-term neuroprotective effects of scalp acupuncture combined with treadmill training on pMCAO rats. |
| 34804173 |
287847 |
Rbfox3
|
Immunity |
The double immunofluorescence staining results showed that multitudinous cIAP1 and NeuN positive neurons existed in the cortex penumbra of the ischemic brain in the sham-operated group (Figure 4). |
| 34757591 |
24505 |
Ins1
|
Immunity |
On the basis of the findings obtained with the observation of insulin in rat pancreas, we further evaluated the results of immunofluorescence staining and identified the expression of TRPV1 and insulin in rat pancreas to explore the neural regulation of pancreatic endocrine secretion through EA (Fig. |
| 34757591 |
24835 |
Tnf
|
Immunity |
TNF-alpha is an inflammatory cytokine produced by various cells, including immune cells and epithelial cells. |
| 34757591 |
83810 |
Trpv1
|
Immunity |
On the basis of the findings obtained with the observation of insulin in rat pancreas, we further evaluated the results of immunofluorescence staining and identified the expression of TRPV1 and insulin in rat pancreas to explore the neural regulation of pancreatic endocrine secretion through EA (Fig. |
| 30341024 |
83817 |
Ache
|
Immunity |
The levels of acetylcholine (ACh), acetylcholinesterase (AChE), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. |
| 30341024 |
24498 |
Il6
|
Immunity |
The levels of acetylcholine (ACh), acetylcholinesterase (AChE), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. |
| 30341024 |
24514 |
Jak2
|
Immunity |
The mRNA and protein expression and immunoreactivity of alpha7nAChR and its postreceptor inflammation signal pathway, including janus kinase 2 (JAK2), signal transducers and activators of transcription 3 (STAT3), nuclear factor-kappaB (NF-kappaB), were observed by quantitative reverse transcription-polymerase chain reaction, Western blot and immunohistochemistry. |
| 30341024 |
25125 |
Stat3
|
Immunity |
The mRNA and protein expression and immunoreactivity of alpha7nAChR and its postreceptor inflammation signal pathway, including janus kinase 2 (JAK2), signal transducers and activators of transcription 3 (STAT3), nuclear factor-kappaB (NF-kappaB), were observed by quantitative reverse transcription-polymerase chain reaction, Western blot and immunohistochemistry. |
| 30341024 |
24835 |
Tnf
|
Immunity |
The levels of acetylcholine (ACh), acetylcholinesterase (AChE), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay. |
| 35216533 |
24185 |
Akt1
|
Immunity |
Then, microvessel density, protein levels and mRNA expression of selected VEGF/PI3K/Akt pathway intermediates were determined by immunofluorescence, immunohistochemistry and Western blot analysis, and mRNA qRT-PCR, respectively. |
| 35216533 |
83785 |
Vegfa
|
Immunity |
Then, microvessel density, protein levels and mRNA expression of selected VEGF/PI3K/Akt pathway intermediates were determined by immunofluorescence, immunohistochemistry and Western blot analysis, and mRNA qRT-PCR, respectively. |
| 35216533 |
170911 |
Pik3ca
|
Immunity |
Then, microvessel density, protein levels and mRNA expression of selected VEGF/PI3K/Akt pathway intermediates were determined by immunofluorescence, immunohistochemistry and Western blot analysis, and mRNA qRT-PCR, respectively. |
| 33715422 |
25325 |
Il10
|
Immunity |
IL-17, tumor necrosis factor (TNF)-alpha, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) and in plasma. |
| 33715422 |
301289 |
Il17a
|
Immunity |
IL-17, tumor necrosis factor (TNF)-alpha, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) and in plasma. |
| 33715422 |
312679 |
Il17ra
|
Immunity |
The expression levels of IL-17R were assayed in lung tissue by real-time polymerase chain reaction (PCR), western blotting, and immunohistochemistry. |
| 33715422 |
24835 |
Tnf
|
Immunity |
IL-17, tumor necrosis factor (TNF)-alpha, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) and in plasma. |
| 31737064 |
58959 |
Crhr1
|
Immunity |
CRF-R1 immunoreactivity within cell bodies showed green cytoplasmic staining, and positive IMMC staining was observed in red, while yellow was the double staining of CRF-R1 and IMMC, as demonstrated in Figure 4(b). |
| 35003293 |
116562 |
Il2
|
Immunity |
The serum levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-2 in OA rats were assessed by enzyme-linked immunosorbent assay (ELISA). |
| 35003293 |
24835 |
Tnf
|
Immunity |
The serum levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-2 in OA rats were assessed by enzyme-linked immunosorbent assay (ELISA). |
| 37383281 |
50689 |
Mapk3
|
Immunity |
The ERK/NF-kappaB signaling pathway is closely related to the body's immune system. |
| 37383281 |
116590 |
Mapk1
|
Immunity |
The ERK/NF-kappaB signaling pathway is closely related to the body's immune system. |
| 37383281 |
50689 |
Mapk3
|
Immunity |
The protein expression levels of the ERK/NF-kappaB signaling pathway related genes were detected by Western blot and immunohistochemistry, and the results were consistent with the trend in mRNA expression (Figure 5). |
| 37383281 |
116590 |
Mapk1
|
Immunity |
The protein expression levels of the ERK/NF-kappaB signaling pathway related genes were detected by Western blot and immunohistochemistry, and the results were consistent with the trend in mRNA expression (Figure 5). |
| 37383281 |
81736 |
Nfkb1
|
Immunity |
The ERK/NF-kappaB signaling pathway is closely related to the body's immune system. |
| 37383281 |
81736 |
Nfkb1
|
Immunity |
The protein expression levels of the ERK/NF-kappaB signaling pathway related genes were detected by Western blot and immunohistochemistry, and the results were consistent with the trend in mRNA expression (Figure 5). |
| 37383281 |
50689 |
Mapk3
|
Immunity |
Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB. |
| 37383281 |
116590 |
Mapk1
|
Immunity |
Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB. |
| 37383281 |
58960 |
Map2k2
|
Immunity |
Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB. |
| 37383281 |
24703 |
Raf1
|
Immunity |
Western blot and immunohistochemistry were carried out to evaluate the protein expression levels of RAF-1, MEK-2, ERK1/2 and NF-kappaB. |
| 35704659 |
59301 |
Ghrl
|
Immunity |
Given that the arcuate nucleus (ARC) is a key site of the reproductive regulatory system and the energy regulating system, therefore, we performed an immunohistochemistry test to determine the expression of NPY, GHRL and their receptor in the ARC. |
| 35704659 |
59301 |
Ghrl
|
Immunity |
Meanwhile, the number of GHRL-positive cells in the ARC in group M was much lower than the number in groups N and EA (P < 0.01, P < 0.05, respectively), but there was no difference between groups M and SA (Fig 5).There was some attenuated immunoreactivity of NPY2R in the ARC in group M compared with group N (P < 0.05), but when treated with EA or SA, the immunoreactivity of NPY2R was enhanced remarkably (P < 0.01, P < 0.05, respectively) (Fig 5). |
| 35704659 |
84022 |
Ghsr
|
Immunity |
As shown by immunofluorescence, the expression of GHSR in the ARC was not significantly different in the four groups. |
| 35704659 |
24604 |
Npy
|
Immunity |
Given that the arcuate nucleus (ARC) is a key site of the reproductive regulatory system and the energy regulating system, therefore, we performed an immunohistochemistry test to determine the expression of NPY, GHRL and their receptor in the ARC. |
| 35704659 |
66024 |
Npy2r
|
Immunity |
Meanwhile, the number of GHRL-positive cells in the ARC in group M was much lower than the number in groups N and EA (P < 0.01, P < 0.05, respectively), but there was no difference between groups M and SA (Fig 5).There was some attenuated immunoreactivity of NPY2R in the ARC in group M compared with group N (P < 0.05), but when treated with EA or SA, the immunoreactivity of NPY2R was enhanced remarkably (P < 0.01, P < 0.05, respectively) (Fig 5). |
| 32547407 |
29583 |
Pecam1
|
Immunity |
Ovarian tissues were made transparent following the CUBIC 3D tissue-clearing protocol and were immunostained using antibodies against platelet endothelial cell adhesion molecule-1 and tyrosine hydroxylase to visualize the ovarian vasculature and innervation, respectively. |
| 32547407 |
25085 |
Th
|
Immunity |
Ovarian tissues were made transparent following the CUBIC 3D tissue-clearing protocol and were immunostained using antibodies against platelet endothelial cell adhesion molecule-1 and tyrosine hydroxylase to visualize the ovarian vasculature and innervation, respectively. |
| 36440366 |
56718 |
Mtor
|
Immunity |
To further confirm the relationship between neuroplasticity-related proteins and the mTOR pathway, we detected the expression of SYN and p-S6 by immunofluorescence (Figures 3(a) and 4(a)). |
| 36440366 |
24949 |
Syn1
|
Immunity |
To further confirm the relationship between neuroplasticity-related proteins and the mTOR pathway, we detected the expression of SYN and p-S6 by immunofluorescence (Figures 3(a) and 4(a)). |
| 36440366 |
29179 |
Syn2
|
Immunity |
To further confirm the relationship between neuroplasticity-related proteins and the mTOR pathway, we detected the expression of SYN and p-S6 by immunofluorescence (Figures 3(a) and 4(a)). |
| 36440366 |
29130 |
Syn3
|
Immunity |
To further confirm the relationship between neuroplasticity-related proteins and the mTOR pathway, we detected the expression of SYN and p-S6 by immunofluorescence (Figures 3(a) and 4(a)). |
| 36440366 |
24949 |
Syn1
|
Immunity |
Double immunofluorescence showed that p-S6 was present around SYN in the healthy cerebral cortex; these markers were even found to be coexpressed. |
| 36440366 |
29179 |
Syn2
|
Immunity |
Double immunofluorescence showed that p-S6 was present around SYN in the healthy cerebral cortex; these markers were even found to be coexpressed. |
| 36440366 |
29130 |
Syn3
|
Immunity |
Double immunofluorescence showed that p-S6 was present around SYN in the healthy cerebral cortex; these markers were even found to be coexpressed. |
| 31419458 |
314322 |
Fos
|
Immunity |
Immunohistochemistry was used to detect Fos-positive nuclei in the ACC. |
| 38178681 |
24185 |
Akt1
|
Immunity |
We measured changes in the ovarian indexes, the number of follicles at all levels, the serum levels of sex hormones and immune factors, the expression levels of phosphoinositide 3-kinase (PI3K), AKT, p-AKT, and caspase-3, and the changes in the proportions of splenic T cell subtypes, including T-helper 17 (Th17), Tc17, regulatory T (Treg), CD4+, and CD8+ cells. |
| 38178681 |
24185 |
Akt1
|
Immunity |
The regulation of sex hormone levels and immune function in rats may be attributed to the adjustment of the mRNA and proteins levels of PI3K, AKT, and caspase-3 in the PI3K/AKT signaling pathway, which leads to an improvement in the immune function of DOR rats. |
| 38178681 |
25402 |
Casp3
|
Immunity |
We measured changes in the ovarian indexes, the number of follicles at all levels, the serum levels of sex hormones and immune factors, the expression levels of phosphoinositide 3-kinase (PI3K), AKT, p-AKT, and caspase-3, and the changes in the proportions of splenic T cell subtypes, including T-helper 17 (Th17), Tc17, regulatory T (Treg), CD4+, and CD8+ cells. |
| 38178681 |
25402 |
Casp3
|
Immunity |
The regulation of sex hormone levels and immune function in rats may be attributed to the adjustment of the mRNA and proteins levels of PI3K, AKT, and caspase-3 in the PI3K/AKT signaling pathway, which leads to an improvement in the immune function of DOR rats. |
| 38178681 |
24932 |
Cd4
|
Immunity |
We measured changes in the ovarian indexes, the number of follicles at all levels, the serum levels of sex hormones and immune factors, the expression levels of phosphoinositide 3-kinase (PI3K), AKT, p-AKT, and caspase-3, and the changes in the proportions of splenic T cell subtypes, including T-helper 17 (Th17), Tc17, regulatory T (Treg), CD4+, and CD8+ cells. |
| 38178681 |
170911 |
Pik3ca
|
Immunity |
The regulation of sex hormone levels and immune function in rats may be attributed to the adjustment of the mRNA and proteins levels of PI3K, AKT, and caspase-3 in the PI3K/AKT signaling pathway, which leads to an improvement in the immune function of DOR rats. |
| 32249904 |
24835 |
Tnf
|
Immunity |
As shown in Figure 4C, in the warm acupuncture-treated group, when compared with the model groups, the proteins with differential levels were enriched in the KEGG pathways of cardiac muscle contraction, endocytosis, platelet activation, actin cytoskeleton regulation, complement and coagulation cascades, apelin signaling, oxytocin signaling, amino acid biosynthesis, autophagy, axon singling, Alzheimer disease, non-alcoholic fatty liver disease (NAFLD), cytokine-cytokine receptor interaction, spliceosome, chemokine signaling, TNF-alpha signaling, neuroactive ligand-receptor interaction, phagocytosis, oxidative phosphorylation and autoimmune thyroid disease (Supplementary Table S8). |
| 32517478 |
303413 |
Mpo
|
Immunity |
Myeloperoxidase (MPO) activity and malondialdehyde (MDA) content in intestinal tissues were assessed using enzyme-linked immunosorbent assay (ELISA) kits. |
| 35321503 |
497229 |
Ang2
|
Immunity |
Immunohistochemical staining for angiogenetic factors indicated the vascularization after CIRI, including VEGF, Ang2, and bFGF. |
| 35321503 |
497229 |
Ang2
|
Immunity |
Immunohistochemical (IHC) Staining of VEGF, Ang2, and bFGF |
| 35321503 |
54250 |
Fgf2
|
Immunity |
Immunohistochemical staining for angiogenetic factors indicated the vascularization after CIRI, including VEGF, Ang2, and bFGF. |
| 35321503 |
83785 |
Vegfa
|
Immunity |
Immunohistochemical staining for angiogenetic factors indicated the vascularization after CIRI, including VEGF, Ang2, and bFGF. |
| 35321503 |
83785 |
Vegfa
|
Immunity |
Immunohistochemical (IHC) Staining of VEGF, Ang2, and bFGF |
| 34630618 |
25125 |
Stat3
|
Immunity |
STAT3 is considered one of the possible substrates of MAPK, which is the common pathway of intracellular signal transmission among different inflammatory cells and various inflammatory mediators and is involved in various biological reactions such as immune response and cell proliferation, differentiation, migration, and apoptosis. |
| 37384285 |
24241 |
Calca
|
Immunity |
The fluorescence intensity of TRPV1-immunoreactive neurons colocalized with CGRP was significantly higher in the WAI-stimulated group (n = 6) than in the normal group (Figures 5A,B) (t-test, F = 3.164, p < 0.001), and the number of TRPV1 neurons double-labeled with IB4 was significantly higher in the WAI group (n = 6) than in the normal group (Figures 5E,F) (n = 6, t-test, F = 1.249, p < 0.05). |
| 37384285 |
83810 |
Trpv1
|
Immunity |
The fluorescence intensity of TRPV1-immunoreactive neurons colocalized with CGRP was significantly higher in the WAI-stimulated group (n = 6) than in the normal group (Figures 5A,B) (t-test, F = 3.164, p < 0.001), and the number of TRPV1 neurons double-labeled with IB4 was significantly higher in the WAI group (n = 6) than in the normal group (Figures 5E,F) (n = 6, t-test, F = 1.249, p < 0.05). |
| 37384285 |
83810 |
Trpv1
|
Immunity |
To observe the activation of TRPV1 by WAI stimulation at 48 C, immunohistochemistry for TRPV1 in the DRGs of C6-T2 was carried out in another set of animals. |
| 37384285 |
83810 |
Trpv1
|
Immunity |
Immunohistochemistry for TRPV1 showed that the enhanced expression of TRPV1 induced by WAI stimulation at PC-6 was suppressed by pretreatment with RTX prior to WAI stimulation (one-way ANOVA; F=13.22, p < 0.0001; Figures 6B,C). |
| 32956833 |
24806 |
Tac1
|
Immunity |
The roles of increased SP in Neuro-Sps were also investigated by using immunohistochemistry, in vivo single-fiber peripheral nerve recordings, and in vivo midbrain extracellular recordings. |
| 32956833 |
24806 |
Tac1
|
Immunity |
To examine the increased release of SP from afferent nerves in Neuro-Sps, immunohistochemical detection of SP was performed on tissue samples from wrist areas of naive (Control) and IMH rats (Fig. |
| 34553631 |
83785 |
Vegfa
|
Immunity |
In addition, EA decreased the aortic angiogenesis signaling cascade, reflected by down-regulation of vascular endothelial growth factor (VEGF) abundance and transforming growth factor beta receptor (TGFbetaR)I/II expression, as assessed by immunostaining. |
| 35245503 |
171373 |
Slc12a5
|
Immunity |
neurologic function scale, muscular tension scale, foot balance test, and gait analysis), H-reflex recording, TTC, Western blotting, RT-qPCR, ELISA, and immunofluorescence molecular assay, the study results illustrated that acupuncture could significantly alleviate the spinal hyperreflexia, decrease muscle tone, and enhance locomotor function by elevating the GABA, KCC2, and GABAAgamma2 expressions in the lumbar spine of a rat model of post-ischemic stroke with spastic hypertonia. |
| 34405366 |
25319 |
Fth1
|
Immunity |
The frequencies of Nrf2, GPX4 and FTH1 positive cells, respectively, were documented with immunohistochemical staining. |
| 34405366 |
25319 |
Fth1
|
Immunity |
In order to determine whether SA therapy elevates FTH1 and GPX4 concentrations via nuclear Nrf2 accretion, immunohistochemical techniques were utilised to assay cerebral tissue Nrf2 immunopositivity. |
| 34405366 |
25319 |
Fth1
|
Immunity |
Concomitantly, the immunopositivity data with respect to GPX4 and FTH1 were in keeping with the changes in the Nrf2 titres (Fig. |
| 34405366 |
25319 |
Fth1
|
Immunity |
Western blotting was used to assay the degree of protein expression of both Nrf2 and the downstream transcripts, GPX4 and FTH1; the results supported the immunopositivity data (Fig. |
| 34405366 |
29328 |
Gpx4
|
Immunity |
The frequencies of Nrf2, GPX4 and FTH1 positive cells, respectively, were documented with immunohistochemical staining. |
| 34405366 |
29328 |
Gpx4
|
Immunity |
Immunopositivity for GPX4 implied that GPX4 levels diminished 6 h and 3 days following ICH but rose following therapy with SA (Fig. |
| 34405366 |
29328 |
Gpx4
|
Immunity |
In order to determine whether SA therapy elevates FTH1 and GPX4 concentrations via nuclear Nrf2 accretion, immunohistochemical techniques were utilised to assay cerebral tissue Nrf2 immunopositivity. |
| 34405366 |
29328 |
Gpx4
|
Immunity |
Concomitantly, the immunopositivity data with respect to GPX4 and FTH1 were in keeping with the changes in the Nrf2 titres (Fig. |
| 34405366 |
29328 |
Gpx4
|
Immunity |
Western blotting was used to assay the degree of protein expression of both Nrf2 and the downstream transcripts, GPX4 and FTH1; the results supported the immunopositivity data (Fig. |
| 34405366 |
83619 |
Nfe2l2
|
Immunity |
The frequencies of Nrf2, GPX4 and FTH1 positive cells, respectively, were documented with immunohistochemical staining. |
| 34405366 |
83619 |
Nfe2l2
|
Immunity |
In order to determine whether SA therapy elevates FTH1 and GPX4 concentrations via nuclear Nrf2 accretion, immunohistochemical techniques were utilised to assay cerebral tissue Nrf2 immunopositivity. |
| 34405366 |
83619 |
Nfe2l2
|
Immunity |
Concomitantly, the immunopositivity data with respect to GPX4 and FTH1 were in keeping with the changes in the Nrf2 titres (Fig. |
| 34405366 |
83619 |
Nfe2l2
|
Immunity |
Western blotting was used to assay the degree of protein expression of both Nrf2 and the downstream transcripts, GPX4 and FTH1; the results supported the immunopositivity data (Fig. |
| 30106055 |
64171 |
Card9
|
Immunity |
Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9. |
| 30106055 |
64171 |
Card9
|
Immunity |
Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1beta. |
| 30106055 |
64171 |
Card9
|
Immunity |
Immunopositivity of Mincle, Syk, and CARD9 in the brain as detected by immunohistochemistry |
| 30106055 |
64171 |
Card9
|
Immunity |
Immunopositivity of Syk and CARD9 were significantly reduced in the acupuncture and piceatannol groups compared with the ICH group (P < 0.05). |
| 30106055 |
64171 |
Card9
|
Immunity |
Immunopositivity of Syk and CARD9 showed no statistical differences between the acupuncture and piceatannol groups (P > 0.05). |
| 30106055 |
450223 |
Clec4e
|
Immunity |
Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9. |
| 30106055 |
450223 |
Clec4e
|
Immunity |
Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1beta. |
| 30106055 |
450223 |
Clec4e
|
Immunity |
Immunopositivity of Mincle, Syk, and CARD9 in the brain as detected by immunohistochemistry |
| 30106055 |
450223 |
Clec4e
|
Immunity |
Compared with the ICH group, Mincle immunopositivity was decreased in the acupuncture group (P < 0.05). |
| 30106055 |
450223 |
Clec4e
|
Immunity |
However, Mincle immunopositivity was not significantly different between the ICH and piceatannol groups (P > 0.05; Figure 4). |
| 30106055 |
25155 |
Syk
|
Immunity |
Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Mincle, Syk, and CARD9. |
| 30106055 |
25155 |
Syk
|
Immunity |
Our results showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARD9, and interkeukin-1beta. |
| 30106055 |
25155 |
Syk
|
Immunity |
Immunopositivity of Mincle, Syk, and CARD9 in the brain as detected by immunohistochemistry |
| 30106055 |
25155 |
Syk
|
Immunity |
Immunopositivity of Syk and CARD9 were significantly reduced in the acupuncture and piceatannol groups compared with the ICH group (P < 0.05). |
| 30106055 |
25155 |
Syk
|
Immunity |
Immunopositivity of Syk and CARD9 showed no statistical differences between the acupuncture and piceatannol groups (P > 0.05). |
| 36484920 |
29248 |
Tnni3
|
Immunity |
Enzyme-linked immunosorbent assay (ELISA) was used to detect creatine kinase isoenzyme MB (CK-MB) and cardiac troponins I (CTnI) levels in serum and superoxide dismutase (SOD) and malondialdehyde (MDA) levels in myocardial tissue. |
| 32021397 |
24225 |
Bdnf
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
24241 |
Calca
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
312701 |
Cd163
|
Immunity |
To observe dural mast cells (Figure 4) and macrophages after dural stimulation (Figure 5), we analyzed immunofluorescence for tryptase and CD163 to measure mast cell and macrophage levels, respectively. |
| 32021397 |
29527 |
Ptgs2
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
24494 |
Il1b
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
24498 |
Il6
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
24835 |
Tnf
|
Immunity |
Serum calcitonin gene-related peptide, cyclooxygenase-2, brain-derived neurotrophic factor, IL-1beta, IL-6, and TNF levels were determined using enzyme-linked immunosorbent assays to evaluate the anti-inflammatory effect of acupuncture. |
| 32021397 |
54271 |
Tpsab1
|
Immunity |
To observe dural mast cells (Figure 4) and macrophages after dural stimulation (Figure 5), we analyzed immunofluorescence for tryptase and CD163 to measure mast cell and macrophage levels, respectively. |
| 33388690 |
362119 |
C5
|
Immunity |
The average increases in the superficial temperature of wounded animals after acupuncture were about 1-2 C. Through the activation of C3a and C5a, the increased secretion of cytokines SDF-1 and TGFbeta-1, as well as the down-regulation of proinflammatory cytokines TNF-alpha and IL-1beta, the combined treatment of stem cell-seeded cryogel/hydrogel biomaterials and acupuncture on wounds produced synergistic immunomodulatory effects. |
| 33388690 |
24494 |
Il1b
|
Immunity |
The average increases in the superficial temperature of wounded animals after acupuncture were about 1-2 C. Through the activation of C3a and C5a, the increased secretion of cytokines SDF-1 and TGFbeta-1, as well as the down-regulation of proinflammatory cytokines TNF-alpha and IL-1beta, the combined treatment of stem cell-seeded cryogel/hydrogel biomaterials and acupuncture on wounds produced synergistic immunomodulatory effects. |
| 33388690 |
24772 |
Cxcl12
|
Immunity |
The average increases in the superficial temperature of wounded animals after acupuncture were about 1-2 C. Through the activation of C3a and C5a, the increased secretion of cytokines SDF-1 and TGFbeta-1, as well as the down-regulation of proinflammatory cytokines TNF-alpha and IL-1beta, the combined treatment of stem cell-seeded cryogel/hydrogel biomaterials and acupuncture on wounds produced synergistic immunomodulatory effects. |
| 33388690 |
59086 |
Tgfb1
|
Immunity |
The average increases in the superficial temperature of wounded animals after acupuncture were about 1-2 C. Through the activation of C3a and C5a, the increased secretion of cytokines SDF-1 and TGFbeta-1, as well as the down-regulation of proinflammatory cytokines TNF-alpha and IL-1beta, the combined treatment of stem cell-seeded cryogel/hydrogel biomaterials and acupuncture on wounds produced synergistic immunomodulatory effects. |
| 33388690 |
24835 |
Tnf
|
Immunity |
The average increases in the superficial temperature of wounded animals after acupuncture were about 1-2 C. Through the activation of C3a and C5a, the increased secretion of cytokines SDF-1 and TGFbeta-1, as well as the down-regulation of proinflammatory cytokines TNF-alpha and IL-1beta, the combined treatment of stem cell-seeded cryogel/hydrogel biomaterials and acupuncture on wounds produced synergistic immunomodulatory effects. |
| 36655632 |
114215 |
Insl3
|
Immunity |
In addition, there was a significant increase in the Johnsen scores, seminiferous tubule diameters, serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone, proliferation indices, and sex hormone-binding globulin (SHBG) and insulin-like peptide 3 (INSL3) immunoreactivities. |
| 36655632 |
24775 |
Shbg
|
Immunity |
In addition, there was a significant increase in the Johnsen scores, seminiferous tubule diameters, serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone, proliferation indices, and sex hormone-binding globulin (SHBG) and insulin-like peptide 3 (INSL3) immunoreactivities. |
| 37548586 |
24245 |
Camk2b
|
Immunity |
We used WB, immunofluorescence, and qRT-PCR to detect the expression of CaMKII/CREB/BDNF signaling pathway-related factors in the synaptic of rat spinal cord in each group. |
| 37548586 |
24245 |
Camk2b
|
Immunity |
The expressions of CAMKII, CREB, and P-CREB in the spinal cord tissues were detected by WB, PCR, and immunohistochemistry. |
| 37548586 |
24245 |
Camk2b
|
Immunity |
Immunofluorescence analysis showed that the staining density of CAMKII was weaker in the EA group compared with the CSR group, but no significant difference was seen in the staining density of CREB and P-CREB in each group. |
| 37548586 |
24225 |
Bdnf
|
Immunity |
We used WB, immunofluorescence, and qRT-PCR to detect the expression of CaMKII/CREB/BDNF signaling pathway-related factors in the synaptic of rat spinal cord in each group. |
| 37548586 |
81646 |
Creb1
|
Immunity |
We used WB, immunofluorescence, and qRT-PCR to detect the expression of CaMKII/CREB/BDNF signaling pathway-related factors in the synaptic of rat spinal cord in each group. |
| 37548586 |
314322 |
Fos
|
Immunity |
We used WB and immunofluorescence method to detect c-fos expression in the spinal cord tissues of rat spinal cord in each group. |
| 37548586 |
314322 |
Fos
|
Immunity |
Immunofluorescence analysis showed that the staining density of c-fos was weaker in the EA group compared to the CSR group. |
| 37548586 |
81646 |
Creb1
|
Immunity |
The expressions of CAMKII, CREB, and P-CREB in the spinal cord tissues were detected by WB, PCR, and immunohistochemistry. |
| 37548586 |
81646 |
Creb1
|
Immunity |
Immunofluorescence analysis showed that the staining density of CAMKII was weaker in the EA group compared with the CSR group, but no significant difference was seen in the staining density of CREB and P-CREB in each group. |
| 37548586 |
117096 |
Nlgn2
|
Immunity |
We used WB and immunofluorescence method to detect NLGN2 expression in the synaptic tissue of rat spinal cord in each group. |
| 37548586 |
117096 |
Nlgn2
|
Immunity |
Immunofluorescence analysis showed that the staining density of NLGN2 was weaker in the EA group compared to the CSR group. |
| 36422883 |
362245 |
Map1lc3a
|
Immunity |
Immunofluorescence was used to detect the LC3-positive cell rate. |
| 36422883 |
362245 |
Map1lc3a
|
Immunity |
To confirm this hypothesis, we used the immunofluorescence assay to detect the LC3 positive cell rate of neurons in the injured side of the cerebral cortex, and the Western blot assay to measure the protein expression of autophagy marker LC3 and autophagy substrate p62. |
| 36422883 |
56718 |
Mtor
|
Immunity |
Co-immunoprecipitation (CO-IP) method was used to identify the protein interaction of mTOR and ULK1. |
| 36422883 |
113894 |
Sqstm1
|
Immunity |
To confirm this hypothesis, we used the immunofluorescence assay to detect the LC3 positive cell rate of neurons in the injured side of the cerebral cortex, and the Western blot assay to measure the protein expression of autophagy marker LC3 and autophagy substrate p62. |
| 36422883 |
360827 |
Ulk1
|
Immunity |
Co-immunoprecipitation (CO-IP) method was used to identify the protein interaction of mTOR and ULK1. |
| 36046956 |
301059 |
Myd88
|
Immunity |
We used Nissl staining to observe the pathological changes in brain tissue, flow cytometry to detect the proportion of M1 and M2 polarized microglia in the injured area on the first, third and fifth day, and co-immunoprecipitation (Co-IP) to examine TLR4/TRIF/MyD88 expression in microglia on the first, third and fifth day, as well as expression of the amount of binding of TLR4 with TRIF and MyD88. |
| 36046956 |
29260 |
Tlr4
|
Immunity |
We used Nissl staining to observe the pathological changes in brain tissue, flow cytometry to detect the proportion of M1 and M2 polarized microglia in the injured area on the first, third and fifth day, and co-immunoprecipitation (Co-IP) to examine TLR4/TRIF/MyD88 expression in microglia on the first, third and fifth day, as well as expression of the amount of binding of TLR4 with TRIF and MyD88. |
| 36046956 |
94196 |
Rnf138
|
Immunity |
We used Nissl staining to observe the pathological changes in brain tissue, flow cytometry to detect the proportion of M1 and M2 polarized microglia in the injured area on the first, third and fifth day, and co-immunoprecipitation (Co-IP) to examine TLR4/TRIF/MyD88 expression in microglia on the first, third and fifth day, as well as expression of the amount of binding of TLR4 with TRIF and MyD88. |
| 35928244 |
306574 |
Slc25a15
|
Immunity |
Results from immunohistostaining revealed the distribution of Slc25a15, a key gene of the mitochondria and mitochondrial energy metabolism. |
| 35722406 |
116996 |
Il16
|
Immunity |
Immunochemistry (IHC), quantitative reverse transcription polymerase chain reaction (qRT-PCR), and western blot analysis were performed to detect the expression level of miR-81, IL-16, and PSD-95. |
| 35722406 |
29495 |
Dlg4
|
Immunity |
Immunochemistry (IHC), quantitative reverse transcription polymerase chain reaction (qRT-PCR), and western blot analysis were performed to detect the expression level of miR-81, IL-16, and PSD-95. |
| 29623933 |
310738 |
Ngf
|
Immunity |
We used immunohistochemistry to observe changes in the expression of S100:a specific marker for Schwann cells:and an enzyme-linked immunosorbent assay to detect serum level of nerve growth factor. |
| 29623933 |
310738 |
Ngf
|
Immunity |
Results showed that compared with the model-only group, sciatic functional index, recovery rate of conduction velocity, diameter recovery of the gastrocnemius muscle fibers, number of S100-immunoreactive cells, and level of nerve growth factor were greater in the electroacupuncture and moxibustion groups. |
| 30884162 |
156117 |
Casp12
|
Immunity |
Immunofluorescence and western blot analysis were used to determine GRP78 and caspase-12 levels in sciatic nerves. |
| 30884162 |
25617 |
Hspa5
|
Immunity |
Immunofluorescence and western blot analysis were used to determine GRP78 and caspase-12 levels in sciatic nerves. |
| 35069759 |
|
|
Immunity |
The expression of EOS, mast cells (MCs), eosinophil peroxidase (EPO), and eosinophil cationic protein (ECP) was assessed by hematoxylin-eosin staining (HE), Toluidine Blue staining (TB), and immunohistochemistry, respectively. |
| 35069759 |
|
|
Immunity |
HE, TB staining, and immunohistochemistry experiments showed that the increased expression of EOS, MCs, EPO, and ECP in uterine tissues was reversed by TN. |
| 35069759 |
|
|
Immunity |
Immunohistochemical staining results showed the positive expression of EPO (Figures 6(a)) and ECP (Figure 6(b)) of all groups in rat uterus. |
| 35069759 |
303414 |
Epx
|
Immunity |
The expression of EOS, mast cells (MCs), eosinophil peroxidase (EPO), and eosinophil cationic protein (ECP) was assessed by hematoxylin-eosin staining (HE), Toluidine Blue staining (TB), and immunohistochemistry, respectively. |
| 35069759 |
303414 |
Epx
|
Immunity |
HE, TB staining, and immunohistochemistry experiments showed that the increased expression of EOS, MCs, EPO, and ECP in uterine tissues was reversed by TN. |
| 35069759 |
303414 |
Epx
|
Immunity |
Immunohistochemical staining results showed the positive expression of EPO (Figures 6(a)) and ECP (Figure 6(b)) of all groups in rat uterus. |
| 34564665 |
29527 |
Ptgs2
|
Immunity |
Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B). |
| 34564665 |
29527 |
Ptgs2
|
Immunity |
Immunohistochemistry results also showed that after MSU injection, the number of iNOS- and COX-2-immunoreactive cells was increased in the synovial tissue of the ankle joint (Figure 4F). |
| 34564665 |
24494 |
Il1b
|
Immunity |
Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B). |
| 34564665 |
24498 |
Il6
|
Immunity |
Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B). |
| 34564665 |
24599 |
Nos2
|
Immunity |
Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B). |
| 34564665 |
24599 |
Nos2
|
Immunity |
Immunohistochemistry results also showed that after MSU injection, the number of iNOS- and COX-2-immunoreactive cells was increased in the synovial tissue of the ankle joint (Figure 4F). |
| 34564665 |
24835 |
Tnf
|
Immunity |
Therefore, we examined the effect of BV on blood cell infiltration and expression of inflammatory mediators by reverse transcriptase-polymerase chain reaction (PCR), western blotting, and immunohistochemistry at 24 h after MSU injection, and found that the messenger RNAs levels of TNF-alpha, IL-1beta, IL6, COX-2, and iNOS (at 24 h) were significantly upregulated after MSU injection, but BV and Col significantly reduced their levels as compared with that of MSU injected rats (n = 3) (Figure 4A,B). |
| 30002000 |
83497 |
Ocln
|
Immunity |
The intestinal epithelial cells were observed under transmission electron microscopy (TEM), while protein expression of occludin was measured by immunohistochemistry and Western blotting. |
| 30002000 |
83497 |
Ocln
|
Immunity |
Protein expression of occludin, reflected by immunohistochemistry scores (IHS) and the results of Western blotting, were significantly reduced in the CLP, CLP+EA and CLP+Sham-EA groups when compared with the Control, Sham-CLP and Control+EA groups (P<0.01). |
| 30883553 |
26198 |
mt-Co2
|
Immunity |
The main outcome measures including paw circumference, paw withdrawal threshold, histopathology and immunoassays of tumor necrosis factor-alpha (TNF-alpha), collagen type II (CoII), cartilage oligomeric matrix protein (COMP) were analyzed. |
| 30883553 |
26198 |
mt-Co2
|
Immunity |
CoII immunohistochemistry analysis of the cartilage from the vehicle-treated side and CFA-injection side of the sLA and LA groups are shown in Fig 4. |
| 30883553 |
26198 |
mt-Co2
|
Immunity |
The CoII-like immunoreactivity of the vehicle-treated sides was relatively normal compared with that of the surrounding tissue, indicating much more type II collagen protein was formed (Fig 4E). |
| 30883553 |
25304 |
Comp
|
Immunity |
The main outcome measures including paw circumference, paw withdrawal threshold, histopathology and immunoassays of tumor necrosis factor-alpha (TNF-alpha), collagen type II (CoII), cartilage oligomeric matrix protein (COMP) were analyzed. |
| 30883553 |
25304 |
Comp
|
Immunity |
Immunofluorescent staining revealed the intensity of COMP-like immunoreactivity on the vehicle-treated side (Fig 5A) was higher compared to the CFA-treated side of the sLA (Fig 5B) and LA (Fig 5C) groups (Scheffe's method, P < 0.001). |
| 30883553 |
25304 |
Comp
|
Immunity |
LA significantly enhanced the ECM with COMP-like immunoreactivity in the cartilage compared with the sLA group (Scheffe's method, P < 0.001, Fig 5D). |
| 30883553 |
24835 |
Tnf
|
Immunity |
The main outcome measures including paw circumference, paw withdrawal threshold, histopathology and immunoassays of tumor necrosis factor-alpha (TNF-alpha), collagen type II (CoII), cartilage oligomeric matrix protein (COMP) were analyzed. |
| 30883553 |
24835 |
Tnf
|
Immunity |
Significantly lower levels of TNF-alpha were found on the vehicle-treated side using immunofluorescent staining (Fig 5A). |
| 30883553 |
24835 |
Tnf
|
Immunity |
It also revealed more abundant expressions of TNF-alpha-like immunoreactivities in the sLA group (Fig 5B) compared with those in the LA group (Fig 5C). |
| 36522251 |
314322 |
Fos
|
Immunity |
Finally, the activities of brain regions related to autonomic nerve regulation were assessed by c-Fos immunofluorescence and multichannel recording. |
| 37212035 |
100360880 |
Fosb
|
Immunity |
Additionally, the content of DeltaFosB, a marker of neuronal activation, in the rat striatum was detected by immunohistochemistry and qRT-PCR. |
| 38036019 |
304729 |
Gli2
|
Immunity |
(Polymerase Chain Reaction) PCR and (Immunohistochemistry) IHC were used to test the changes in Shh, Ptch, Smo, and Gli2 mRNA and proteins. |
| 38036019 |
89830 |
Ptch1
|
Immunity |
(Polymerase Chain Reaction) PCR and (Immunohistochemistry) IHC were used to test the changes in Shh, Ptch, Smo, and Gli2 mRNA and proteins. |
| 38036019 |
29499 |
Shh
|
Immunity |
(Polymerase Chain Reaction) PCR and (Immunohistochemistry) IHC were used to test the changes in Shh, Ptch, Smo, and Gli2 mRNA and proteins. |
| 38036019 |
25273 |
Smo
|
Immunity |
(Polymerase Chain Reaction) PCR and (Immunohistochemistry) IHC were used to test the changes in Shh, Ptch, Smo, and Gli2 mRNA and proteins. |
| 30872987 |
29545 |
Uchl1
|
Immunity |
On the other hand, in a quantitative immunohistochemical analysis for PGP 9.5, a marker for nerve fibers (Beiswenger et al.,), the morphological changes of the peripheral nerve were not observed in collagenase-treated skin compared to the normal group (Figures 5C,D). |
| 30069225 |
25712 |
Ifng
|
Immunity |
In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-gamma, TLR2, and TLR4. |
| 30069225 |
299351 |
Ighe
|
Immunity |
Allergic rhinitis (AR), an IgE-mediated response, is characterized by a Th2-type immunological pattern together with mast cells activation. |
| 30069225 |
287287 |
Il4
|
Immunity |
In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-gamma, TLR2, and TLR4. |
| 30069225 |
310553 |
Tlr2
|
Immunity |
In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-gamma, TLR2, and TLR4. |
| 30069225 |
310553 |
Tlr2
|
Immunity |
In our immunofluorescence analysis (Figure 5), we could not detect any significant difference in the expression of TLR2 in the nasal mucosa among the C1, C2, model, and sham groups (P>0.05). |
| 30069225 |
29260 |
Tlr4
|
Immunity |
In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-gamma, TLR2, and TLR4. |
| 30069225 |
24806 |
Tac1
|
Immunity |
In the present study, we have further used this model to address the immunomodulatory effects of CIAA stimulation at Yingxiang (LI20) and Zusanli (ST36) acupoints and to elucidate the mechanisms involved in the regulation of SP, sIgE, IL-4, IFN-gamma, TLR2, and TLR4. |
| 36819511 |
100314241 |
Mir146a
|
Immunity |
The anti-inflammatory mechanism of acupuncture was studied by using the expressions of microRNA-146a (miR-146a) mediated nuclear factor-kappa B (NF-kappaB) signaling pathway-related proteins were detected by immunofluorescence, western blotting, quantitative real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). |
| 36819511 |
100314241 |
Mir146a
|
Immunity |
The anti-inflammatory mechanism of acupuncture was studied by using the expressions of microRNA-146a (miR-146a) mediated nuclear factor-kappa B (NF-kappaB) signaling pathway-related proteins were detected by immunofluorescence, western blotting, quantitative real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). |
| 36819511 |
81736 |
Nfkb1
|
Immunity |
The anti-inflammatory mechanism of acupuncture was studied by using the expressions of microRNA-146a (miR-146a) mediated nuclear factor-kappa B (NF-kappaB) signaling pathway-related proteins were detected by immunofluorescence, western blotting, quantitative real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). |
| 36819511 |
81736 |
Nfkb1
|
Immunity |
NF-kappaB expression in the erector spinae sections of rats was analyzed by immunofluorescence (Figure 5A). |
| 32082125 |
24599 |
Nos2
|
Immunity |
The serum levels of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and iNOS were detected by enzyme linked immunosorbent assay (ELISA). |
| 32082125 |
24835 |
Tnf
|
Immunity |
The serum levels of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and iNOS were detected by enzyme linked immunosorbent assay (ELISA). |
| 34309681 |
24498 |
Il6
|
Immunity |
After observation periods, blood samples for interleukin-6 and beta-endorphin and skin biopsies for inflammatory-changes and immunohistochemical-staining of interleukin-6 were collected for analysis( P< .05 ). |
| 34309681 |
24664 |
Pomc
|
Immunity |
After observation periods, blood samples for interleukin-6 and beta-endorphin and skin biopsies for inflammatory-changes and immunohistochemical-staining of interleukin-6 were collected for analysis( P< .05 ). |
| 34886711 |
81648 |
Crh
|
Immunity |
The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. |
| 34886711 |
24413 |
Nr3c1
|
Immunity |
The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. |
| 34886711 |
24664 |
Pomc
|
Immunity |
The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. |
| 34675986 |
24241 |
Calca
|
Immunity |
The proportion of C-fiber neurons (calcitonin gene-related peptide- (CGRP-) positive neurons) and A-fiber neurons (neurofilament 200- (NF200-) positive neurons) in the dorsal root ganglia (DRG) activated by MA were quantitatively analyzed with the morphological immunofluorescence staining method. |
| 1362034 |
24473 |
Htr1a
|
Immunity |
The present work isl designed to detect the co-localization of Fos protein and serotonin (5-HT) in the neurons of the brainstem in EA treated rats by double labeling immunocytochemistry. |
| 1383896 |
100009275 |
TAC1
|
Immunity |
The effects of electro-acupuncture (EAP) on the release of substance P (SP) and the responses evoked by tooth pulp stimulation (ST) m superlicial layers of the trigeminal nucleus caudalis (Vc-l, ll) were studied in rabbits.ST evoked increase in release of immunoreactive SP (iSP). |
| 7889363 |
24604 |
Npy
|
Immunity |
The effects of single or repeated treatments with manual acupuncture (ACU), electro-acupuncture (ELACU) or physical exercise on neuropeptide Y (NPY), neurokinin A (NKA), substance P (SP), galanin (GAL) and vasoactive intestinal peptide (VIP)-like immunoreactivity (-LI) in different regions of the rat brain were studied. |
| 7889363 |
24806 |
Tac1
|
Immunity |
The effects of single or repeated treatments with manual acupuncture (ACU), electro-acupuncture (ELACU) or physical exercise on neuropeptide Y (NPY), neurokinin A (NKA), substance P (SP), galanin (GAL) and vasoaetive intestinal peptide (VIP)-like immunoreactivity (-LI) in different regions of the rat brain were studied. |
| 7889363 |
29141 |
Gal
|
Immunity |
The effects of single or repeated treatments with manual acupuncture (ACU), electro-acupuncture (ELACU) or physical exercise on neuropeptide Y (NPY), neurokinin A (NKA), substance P (SP), galanin (GAL) and vasoactive intestinal peptide (VIP)-like immunoreactivity (-LI) in different regions of the rat brain were studied. |
| 7889363 |
117064 |
Vip
|
Immunity |
The effects of single or repeated treatments with manual acupuncture (ACU), electro-acupuncture (ELACU) or physical exercise on neuropeptide Y (NPY), neurokinin A (NKA), substance P (SP), galanin (GAL) and vasoactive intestinal peptide (VIP)-like immunoreactivity (-LI) in different regions of the rat brain were studied. |
| 8714708 |
24604 |
Npy
|
Immunity |
The effects of repeated sensory stimulation (electro-acupuncture) and physical exercise (running) on open-field behaviour and on hippocampal concentrations of neuropeptide Y, neurokinin A, substance P, galanin and vasoactive intestinal peptide (VIP)-like immunoreactivities were studied in WKY (wistar-Kyoto) and SHR (spontaneously hypertensive) rats. |
| 8714708 |
24806 |
Tac1
|
Immunity |
The effects of repeated sensory stimulation (electro-acupuncture) and physical exercise (running) on open-field behaviour and on hippocampal concentrations of neuropeptide Y, neurokinin A, substance P, galanin and vasoactive intestinal peptide (VIP)-like immunoreactivities were studied in WKY (wistar-Kyoto) and SHR (spontaneously hypertensive) rats. |
| 8714708 |
24806 |
Tac1
|
Immunity |
The effects of repeated sensory stimulation (electro-acupuncture) and physical exercise (running) on open-field behaviour and on hippocampal concentrations of neuropeptide Y, neurokinin A, substance P, galanin and vasoactive intestinal peptide (VIP)-like immunoreactivities were studied in WKY (wistar-Kyoto) and SHR (spontaneously hypertensive) rats. |
| 8714708 |
29141 |
Gal
|
Immunity |
The effects of repeated sensory stimulation (electro-acupuncture) and physical exercise (running) on open-field behaviour and on hippocampal concentrations of neuropeptide Y, neurokinin A, substance P, galanin and vasoactive intestinal peptide (VIP)-like immunoreactivities were studied in WKY (wistar-Kyoto) and SHR (spontaneously hypertensive) rats. |
| 8714708 |
117064 |
Vip
|
Immunity |
The effects of repeated sensory stimulation (electro-acupuncture) and physical exercise (running) on open-field behaviour and on hippocampal concentrations of neuropeptide Y, neurokinin A, substance P, galanin and vasoactive intestinal peptide (VIP)-like immunoreactivities were studied in WKY (wistar-Kyoto) and SHR (spontaneously hypertensive) rats. |
| 8736578 |
314322 |
Fos
|
Immunity |
No c-fos expression occurred in pituitary cells immunoreactive for growth hormone, prolactin, luteinizing hormone, or thyrotropin-stimulating hormone. |
| 8736578 |
81668 |
Gnrhr
|
Immunity |
No c-fos expression occurred in pituitary cells immunoreactive for growth hormone, prolactin, luteinizing hormone, or thyrotropin-stimulating hormone. |
| 8736578 |
24683 |
Prl
|
Immunity |
No c-fos expression occurred in pituitary cells immunoreactive for growth hormone, prolactin, luteinizing hormone, or thyrotropin-stimulating hormone. |
| 10064107 |
24505 |
Ins1
|
Immunity |
Plasma concentrations of insulin, glucagon and beta-endorphin- were also determined using radioimmunoassay. |
| 10064107 |
24952 |
Gcg
|
Immunity |
Plasma concentrations of insulin, glucagon and beta-endorphin- were also determined using radioimmunoassay. |
| 10064107 |
25601 |
Oprm1
|
Immunity |
Plasma concentrations of insulin, glucagon and beta-endorphin- were also determined using radioimmunoassay. |
| 10830970 |
25601 |
Oprm1
|
Immunity |
Effect of intrathecal morphine and electro-acupuncture on cellular immune function of rats and increment of mu-opioid receptor mRNA expression in PAG following intrathecal morphine |
| 12668152 |
25054 |
Ntrk2
|
Immunity |
In the present study, we investigated the neuroprotective effects of acupuncture on the nigrostriatal system in rat unilaterally lesioned with 6-hydroxydopamine (6-OHDA, 4 microg/microl, intrastriatal injection) using tyrosine hydroxylase (TH) and receptor for brain-derived neurotrophic factor, trkB, immunohistochemistries. |
| 12668152 |
25085 |
Th
|
Immunity |
In the present study, we investigated the neuroprotective effects of acupuncture on the nigrostriatal system in rat unilaterally lesioned with 6-hydroxydopamine (6-OHDA, 4 microg/microl, intrastriatal injection) using tyrosine hydroxylase (TH) and receptor for brain-derived neurotrophic factor, trkB, immunohistochemistries. |
| 12866201 |
83785 |
Vegfa
|
Immunity |
Immunohistochemical study showed a remarkable induction of vascular endothelial growth factor(VEGF) in astrocytes of the peri-infarctarea at 30 days, more in EA treated groups than in groups treated with MCAO alone. |
| 12943179 |
29527 |
Ptgs2
|
Immunity |
Immunohistochemical localization of cyclooxygenase-2 in pregnant rat uterus by Sp-6 acupuncture |
| 14519957 |
24835 |
Tnf
|
Immunity |
The TNF-alpha, IL-1beta and IL-6 positive cells in the immunohistological sections of subchondral bone region of the joint significantly decreased in HP-treated (ST36 acupoint) arthritic group as compared with those in non-treated or HP-treated (non-acupoint) ones, which was coincident with the behavioral studies. |
| 14519957 |
24494 |
Il1b
|
Immunity |
The TNF-alpha, IL-1beta and IL-6 positive cells in the immunohistological sections of subchondral bone region of the joint significantly decreased in HP-treated (ST36 acupoint) arthritic group as compared with those in non-treated or HP-treated (non-acupoint) ones, which was coincident with the behavioral studies. |
| 15633814 |
314322 |
Fos
|
Immunity |
Immobilization stress (180 minutes) preferentially produced a significant increase in Fos-like immunoreactivity (FLI) in stress relevant regions |
| 15777754 |
24598 |
Nos1
|
Immunity |
nNOS immunostaining and NADPH diaphorase reactivity was neither altered in the gracile nucleus and mNTS of non-acupoint stimulated rats nor other brainstem nuclei in rats with EA ST 36. |
| 15777754 |
|
|
Immunity |
nNOS immunostaining and NADPH diaphorase reactivity was neither altered in the gracile nucleus and mNTS of non-acupoint stimulated rats nor other brainstem nuclei in rats with EA ST 36. |
| 16355445 |
314322 |
Fos
|
Immunity |
The aim of the present study was to examine whether acupuncture could affect the postsynaptic neural activations as evaluated by expression of c-fos in the central dopaminergic terminal areas, which are known to be associated with AWS. Therefore, we investigated whether acupuncture at ST36 or SP6 would attenuate AWS, and striatal and accumbal FLI in alcoholized rats utilizing Fos-like immunohistochemistry |
| 16459202 |
24221 |
Avp
|
Immunity |
pain stimulation could change the AVP concentrations only in the rat brain nuclei, not in spinal cord and blood; only intraventricular injection, not intrathecal injection and intravenous injection, of AVP increased the pain threshold, whereas intraventricular injection, not intrathecal injection and intravenous injection, of anti-AVP serum decreased the pain threshold.acupuncture decreased inter-cell AVP, i.e.AVP immunoreactivecells, not OXT, L-Ek, -Ep and DynA1-13 immunoreactive cells in PVH using immunocytochemistry. |
| 17645478 |
100355199 |
CCK
|
Immunity |
Blood plasma and blood serum were isolated to detect motilin and CCK. All samples were tested by the nuclear medicine department at Zhongshan Hospital, where radio immune assay and enzymelinked immunoassay were used to detect motilin and CCK. |
| 20546685 |
116669 |
Vwf
|
Immunity |
The vascular endothelial cell (EC) proliferation were stained by double-immunofluorescence labeling method (Ki67 and vWF), region cerebral blood flow (rCBF) was measured using laser Doppler flowmeter (LDF), and the neurological scores was assessed. |
| 20633459 |
100344462 |
POMC
|
Immunity |
Staining of the skin sections of CV12 with polyclonal antibodies against NA and A remove round brackets revealed the presence of catecholamine-positive cells containing NA, whereas immunoreaction for A was negative. |
| 20953422 |
117064 |
Vip
|
Immunity |
As shown in Figure 8, the VIP-immunostaining intensity was significantly negatively correlated with the scores of inflammation in the synovial tissue (r = -0.483, P = .0026), suggesting that the anti-inflammatory effects of EA at acupoint may be mediated through the up-regulation of VIP expression. |
| 20981331 |
25353 |
Spp1
|
Immunity |
Expression of Endometrial LIF and OPN Proteins in Immunohistochemistry |
| 21643998 |
24221 |
Avp
|
Immunity |
The hypothalamic immunoreactivity (IR) of arginine vasopressin (AVP) was also examined. |
| 28473863 |
100008990 |
IL1B
|
Immunity |
The bacterial endotoxin will act on the mononuclear macrophages and neutrophils, producing and releasing IL-1beta, TNF-alpha, and other inflammatory factors, while generating anti-inflammatory factor IL-4 for immunosuppression. |
