CHIKV Mutation Detail Information

Virus Mutation CHIKV Mutation A226V


Basic Characteristics of Mutations
Mutation Site A226V
Mutation Site Sentence An outbreak of chikungunya in southern Thailand from 2008 to 2009 caused by African strains with A226V mutation.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region E1
Standardized Encoding Gene E1
Genotype/Subtype African
Viral Reference -
Functional Impact and Mechanisms
Disease Chikungunya Fever    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information Y
Treatment -
Location Thailand
Literature Information
PMID 20417142
Title An outbreak of chikungunya in southern Thailand from 2008 to 2009 caused by African strains with A226V mutation
Author Rianthavorn P,Prianantathavorn K,Wuttirattanakowit N,Theamboonlers A,Poovorawan Y
Journal International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
Journal Info 2010 Sep;14 Suppl 3:e161-5
Abstract OBJECTIVES: To elucidate clinical and molecular characteristics of chikungunya fever (CHIK fever) from the 2008-2009 outbreak caused by chikungunya virus (CHIKV) in southern Thailand. METHODS: Three hundred and eighty-one sera from 332 patients with acute febrile illness were tested for anti-CHIKV IgM antibody by ELISA. A molecular analysis of these sera was performed using a semi-nested reverse transcriptase polymerase chain reaction (RT-PCR), followed by direct sequencing and phylogenetic analysis. RESULTS: One hundred and seventy-nine patients were diagnosed with CHIK fever by molecular analysis and/or anti-CHIKV IgM antibody detection. Patients diagnosed with CHIK fever were significantly older than controls (mean age 38.8+/-19 vs. 28.7+/-18 years, p<0.0001) and presented with arthralgia more often than controls. One hundred percent of the sera were positive by RT-PCR, whereas only 10% were positive in serological tests for anti-CHIKV IgM antibody by ELISA if the serum was obtained during the first 4 days of fever. In contrast, CHIKV-specific IgM antibody by ELISA was found in 100% of patients, whereas 15% of patients were positive by RT-PCR if the serum was obtained more than 9 days after the onset of fever. RT-PCR for CHIKV should be performed if the patients present within the first 4 days of fever. Patients presenting after at least 9 days of fever should be tested for IgM antibody. Based on phylogenetic analysis, the CHIKV strains isolated belong to African genotypes harboring the E1 A226V mutation, indicating a single origin of the 2004-2009 CHIKV outbreaks. CONCLUSIONS: The novel CHIKV mutation could potentially modify the epidemiological presentation of CHIK fever. Early diagnosis of CHIK fever is essential for preventing further massive outbreaks.
Sequence Data FJ882857–FJ882922
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.