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Basic Characteristics of Mutations
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Mutation Site
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A594V |
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Mutation Site Sentence
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Table 2 |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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UL97 |
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Standardized Encoding Gene
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UL97
|
|
Genotype/Subtype
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- |
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Viral Reference
|
AD169
|
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Functional Impact and Mechanisms
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Disease
|
Cytomegalovirus infections
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|
Immune
|
- |
|
Target Gene
|
-
|
|
Clinical and Epidemiological Correlations
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|
Clinical Information
|
Y |
|
Treatment
|
GCV |
|
Location
|
Iran |
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Literature Information
|
|
PMID
|
28979335
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Title
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Incidence of Ganciclovir Resistance in CMV-positive Renal Transplant Recipients and its Association with UL97 Gene Mutations
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Author
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Aslani HR,Ziaie S,Salamzadeh J,Zaheri S,Samadian F,Mastoor-Tehrani S
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Journal
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Iranian journal of pharmaceutical research : IJPR
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Journal Info
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2017 Spring;16(2):805-810
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Abstract
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Human cytomegalovirus (CMV) remains the most common infection affecting organ transplant recipients. Despite advances in the prophylaxis and acute treatment of CMV, it remains an important pathogen affecting the short- and long-term clinical outcome of solid organ transplant recipient. The emergence of CMV resistance in a patient reduces the clinical efficacy of antiviral therapy, complicates therapeutic and clinical management decisions, and in some cases results in loss of the allograft and/or death of the patient. Common mechanisms of CMV resistance to ganciclovir have been described chiefly with the UL97 mutations. Here we evaluate Incidence of ganciclovir resistance in 144 CMV-positive renal transplant recipients and its association with UL97 gene mutations. Active CMV infection was monitored by viral DNA quantification in whole blood, and CMV resistance was assessed by UL97 gene sequencing. Six mutations in six patients were detected. Three patients (2.6%) of 112 patients with history of ganciclovir (GCV) treatment had clinical resistance with single UL97 mutations at loci known to be related to resistance (including mutations at codon 594, codon 460, and codon 520). three patients who were anti-CMV drug naive had single UL97 mutations (D605E) without clinical resistance. Our results confirm and extend our earlier findings on the specific mutations in the UL97 phosphotransferase gene in loci that have established role in ganciclovir resistance and also indicate that clinical ganciclovir resistance due to UL97 gene mutations is an issue in subjects with history of with ganciclovir treatment. D605E mutations remains a controversial issue that needs further investigations.
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Sequence Data
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-
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