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Basic Characteristics of Mutations
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Mutation Site
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C1673T |
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Mutation Site Sentence
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Significance of the double mutations of C1673T/C1799G in HBV C promoter |
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Mutation Level
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Nucleotide level |
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Mutation Type
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Synonymous substitution |
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Gene/Protein/Region
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Core Promoter |
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Standardized Encoding Gene
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Genotype/Subtype
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C |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Hepatitis B Virus Infection
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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23547448
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Title
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[Significance of the double mutations of C1673T/C1799G in HBV C promoter]
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Author
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Hu ZY,Yang J,Xia SB,Deng L,Wang YL,Fan L,Shi H
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Journal
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Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
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Journal Info
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2012 Oct;26(5):321-3
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Abstract
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OBJECTIVE: To explore the biological and clinical significance of the double mutations of C1673T/C1799G in HBV C promoter (CP). METHODS: Totally 136 patients were enrolled, including 25 asymptomatic carriers (AsC), 38 patients with chronic hepatitis B (CHB), 24 patients with chronic severe hepatitis B (CSHB), 36 cases with liver cirrhosis (LC) and 13 cases with hepatocellular carcinoma (HCC). HBV subgenotypes and mutations in CP of all samples were determined by nested-PCR and direct nucleotide sequence analysis. The C to T mutation at nucleotide 1673 and C to G at nucleotide 1799 were analyzed in different subgenotypes, and the relationships of C1673T/C1799G double mutations with HBV replication, the expression of HBeAg, and with the severity of liver disease after chronic HBV infection were studied. RESULTS: Of the 136 patients, 110 were subgenotype Ba, 1 was Bj, 7 were C1, and 18 were C2. C1673T/C1799G double mutations in Ba were determined in 106 (96. 4%) samples, which was significantly higher than in C1 (14.3%) and C2 (12.5%) subgenotype (P < 0.0001). In contrast to non-mutation group, HBV DNA content in mutation group had no significant difference (P > 0.05). The prevalence of the mutation was lower in HBeAg positive patients (71.4%) than in HBeAg negative patients (87.5%) (P < 0.05). The frequencies of the double mutations were not significantly different among ASC, CHB, CSHB, LC and HCC groups (P > 0.05). CONCLUSION: In Ba subgenotype, double mutations of C1673T/C1799G is much popular than in C1 and C2; the mutation has no effect on HBV replication, and may not be associated with the outcome of chronic HBV infection.
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Sequence Data
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-
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